[1]贾丽娟,杜苏丰.重组新城疫病毒疫苗rlRVG对A549荷瘤小鼠瘤体生长的抑制作用[J].陕西医学杂志,2019,(1):6-9.
　JIA Lijuan,DU Sufeng..The tumor growth influence of recombinant NDV (rlRVG) on lung adenocarcinoma A549transfected in vivo[J].,2019,(1):6-9.
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重组新城疫病毒疫苗rlRVG对A549荷瘤小鼠瘤体生长的抑制作用
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2019年1期
页码:
6-9
栏目:
基础研究
出版日期:
2019-01-05

文章信息/Info

Title:
The tumor growth influence of recombinant NDV (rlRVG) on lung adenocarcinoma A549transfected in vivo
文章编号:
DOI:10.3969/j.issn.10007377.2019.01.002
作者:
贾丽娟; 杜苏丰
西安市胸科医院呼吸科（西安 710100）
Author(s):
JIA Lijuan ; DU Sufeng.
Xi'an Chest Hospital(Xi'an 710100 ）
关键词:
重组新城疫病毒; 肺癌; 生长抑制; 免疫; 动物实验; 小鼠
Keywords:
Key words RlRVG; Lung cancer; Growth inhibition ; Immune response; Animal experiment; Mouse
分类号:
R734.2
文献标志码:
A
摘要:
摘 要 目的：探讨稳定表达狂犬病毒糖蛋白的重组新城疫病毒(NDV)疫苗(rlRVG)对人肺腺癌A549细胞荷瘤鼠的生长抑制及其可能的免疫机制。方法：建立A549荷瘤鼠模型并随机分为rlRVG组、NDV组和对照组，每组10只，分别于瘤体注射rlRVG，NDV和PBS，每周2次，共3周。测量三组瘤体体积；PCR法测NDV及狂犬病毒糖蛋白(RVG)的表达,蛋白印迹法检测皮下瘤体组织RVG、NDV蛋白表达；流式细胞术检测荷瘤小鼠脾脏自然杀伤细胞(NK)细胞数。结果：与对照组相比，rlRVG组及NDV组荷瘤鼠瘤体增长明显缓慢；NDV在rlRVG组和NDV组中均有表达，而RVG在rlRVG组中表达，对照组未见二者表达；rlRVG组及NDV组瘤体NK细胞数明显增多，且rlRVG组明显高于NDV组。结论：rlRVG成功转染至A549荷瘤鼠，对其瘤体生长有着明显的抑制作用，可能与rlRVG激活机体的免疫反应相关。
Abstract:
Abstract Objective：To explore the effects of recombinant avirulent newcastle disease virus (NDV) LaSota strain expressing the rabies virus glycoprotein (rlRVG) on the proliferation of A549 lung adenocarcinoma tumorbearing mice and its immune mechanism. Methods：A tumor model of human A549 into nude mice was created and divided randomly into rlRVG group,NDV group and control group. The mice in each group received rlRVG,NDV and PBS injection,respectively,twice a week,for 3 weeks. The growth of tumor were recorded,and the expression of NDV and rabies virus glycoprotein，RVG were detected by PCR and Western blot . The number of NK (CD3CD49+) cells were detected by flow cytometry. Results：Compared with control group,the growth of tumor in rlRVG group and NDV group were inhibited more effectively. NDV was expressed in rlRVG group and NDV group,RVG was expressed in rlRVG group,while both two were not expressed in control group. The number of NK cells was significantly higher in rlRVG and NDV groups than in the PBS group. All the phenomenons were more obvious in rlRVG group than that in NDV group. Conclusion：Recombinant rlRVG transfected successfully,and effectively inhibited the growth of A549 lung adenocarcinoma by mechanism which inducing regulating cell immune response.

参考文献/References:

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更新日期/Last Update: 2019-01-22