[1]彭黎,赵海霞,李雅萌.基于NOD样受体热蛋白结构域相关蛋白3炎性小体探讨栀子苷抗肺动脉高压的作用及机制[J].陕西医学杂志,2026,(7):882-887.[doi:DOI:10.3969/j.issn.1000-7377.2026.07.003]
　PENG Li,ZHAO Haixia,LI Yameng.Exploring the effects and mechanism of geniposide against pulmonary arterial hypertension based on the NLRP3 inflammasome[J].,2026,(7):882-887.[doi:DOI:10.3969/j.issn.1000-7377.2026.07.003]
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基于NOD样受体热蛋白结构域相关蛋白3炎性小体探讨栀子苷抗肺动脉高压的作用及机制

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2026年7期
页码:
882-887
栏目:
基础研究
出版日期:
2026-07-05

文章信息/Info

Title:
Exploring the effects and mechanism of geniposide against pulmonary arterial hypertension based on the NLRP3 inflammasome
作者:
彭黎; 赵海霞; 李雅萌
（内蒙古自治区人民医院心血管内科，内蒙古 呼和浩特010010）
Author(s):
PENG Li; ZHAO Haixia; LI Yameng
（Department of Cardiovascular Medicine,Inner Mongolia Autonomous Region People’s Hospital,Hohhot 010010，China）
关键词:
肺动脉高压; 栀子苷; 焦亡; NOD样受体热蛋白结构域相关蛋白3; 炎症因子; 大鼠
Keywords:
Pulmonary arterial hypertension; Geniposide; Pyroptosis; NLRP3; Inflammatory factors; Rat
分类号:
R 543.2
DOI:
DOI:10.3969/j.issn.1000-7377.2026.07.003
文献标志码:
A
摘要:
目的：探究栀子苷对野百合碱（MCT）诱导的肺动脉高压（PAH）大鼠血管重塑的作用及分子机制。方法：30只大鼠利用MCT诱导建立PAH大鼠模型。将PAH大鼠分为模型组、栀子苷低（25 mg/kg）、高剂量（50 mg/kg）组，另取10只正常大鼠为对照组。干预3周后检测大鼠右心室收缩压（RVSP）、平均肺动脉压（mPAP）、右室肥厚指数（RVHI）；苏木精-伊红（HE）、α-平滑肌肌动蛋白（α-SMA）免疫组化及Ki67免疫荧光染色法观察肺血管形态、肌化和细胞增殖情况；酶联免疫吸附测定（ELISA）法检测肺组织肿瘤坏死因子（TNF）-α、白介素（IL）-1β、IL-6水平；Western blot检测肺NOD样受体热蛋白结构域相关蛋白3（NLRP3）、半胱氨酸天冬氨酸蛋白酶-1（Caspase-1）、消皮素D（GSDMD）蛋白表达水平。结果：与对照组比较，模型组大鼠RVSP、mPAP、RVHI水平升高（均P<0.05）；肺组织中血管肌化、Ki67、TNF-α、IL-1β、IL-6及NLRP3、Caspase-1、GSDMD蛋白表达水平升高（均P<0.05）。与模型组比较，栀子苷低、高剂量组大鼠RVSP、mPAP、RVHI水平降低（均P<0.05）；肺组织中血管肌化、Ki67、TNF-α、IL-1β、IL-6及NLRP3、Caspase-1、GSDMD蛋白表达水平降低（均P<0.05）。结论：栀子苷可能通过抑制NLRP3介导的细胞焦亡通路，减轻炎症反应，从而改善PAH大鼠肺血管重塑。
Abstract:
Objective:Investigating the impact and molecular basis of geniposide on vascular restructuring triggered by monocrotaline (MCT) in rats with pulmonary arterial hypertension (PAH).Methods：Thirty rats were employed to develop a PAH model_triggered_by MCT.These PAH-affected rats were categorized into a model cohort,a low-dose geniposide group (25 mg/kg),and a high-dose geniposide group (50 mg/kg).Additionally,ten healthy rats served as the control group.Following a three-week intervention period,rats were evaluated for RVSP,mPAP,and RVHI indices.Hematoxylin-eosin (HE) staining,α-SMA immunohistochemistry,and Ki67 immunofluorescence staining were utilized to examine the pulmonary blood vessel morphology,myogenesis,and cell proliferation,respectively.Utilizing the ELISA technique,we assessed the concentrations of TNF-α,IL-1β,and IL-6 in lung tissue.Furthermore,Western blot analysis was conducted to evaluate the expression levels of NLRP3,Caspase-1,and GSDMD proteins in the lung tissue.Results:Compared with the control group,the levels of RVSP,mPAP,RVHI increased in model group rats (all P<0.05);The expression levels of vascular myogenesis,Ki67,TNF-α,IL-1 β,IL-6,NLRP3,Caspase-1,and GSDMD proteins were elevated in lung tissue (all P<0.05).Compared with the model group,the levels of RVSP,mPAP,and RVHI in rats in the low and high dose groups of geniposide were reduced (all P<0.05);The expression levels of vascularization,Ki67,TNF-α,IL-1β,IL-6,NLRP3,Caspase-1,and GSDMD proteins in lung tissue were reduced (all P<0.05).Conclusion:Geniposide may improve pulmonary vascular remodeling in PAH rats by inhibiting the NLRP3-mediated pyroptosis pathway,reducing inflammatory response,and thus improving pulmonary vascular remodeling in PAH rats.

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备注/Memo

备注/Memo:
内蒙古自治区首府地区公立医院高水平临床专科建设科技项目（2024SGGZ013）
更新日期/Last Update: 2026-07-10