[1]陈正益,刘若一,时立新.鸢尾素对白细胞介素-1β诱导大鼠甲状腺细胞凋亡的影响及机制研究[J].陕西医学杂志,2026,(7):877-881，918.[doi:DOI:10.3969/j.issn.1000-7377.2026.07.002]
　CHEN Zhengyi,LIU Ruoyi,SHI Lixin.Effect and mechanism of Irisin on interleukin-1β induced apoptosis of rat thyroid cells[J].,2026,(7):877-881，918.[doi:DOI:10.3969/j.issn.1000-7377.2026.07.002]
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鸢尾素对白细胞介素-1β诱导大鼠甲状腺细胞凋亡的影响及机制研究

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2026年7期
页码:
877-881，918
栏目:
基础研究
出版日期:
2026-07-05

文章信息/Info

Title:
Effect and mechanism of Irisin on interleukin-1β induced apoptosis of rat thyroid cells
作者:
陈正益1; 刘若一1; 时立新2
（1.贵州医科大学附属医院内分泌科，贵州 贵阳550001；2.贵黔国际总医院内分泌科，贵州 贵阳550018）
Author(s):
CHEN Zhengyi1; LIU Ruoyi1; SHI Lixin2
（1.Department of Endocrinology,Affiliated Hospital of Guizhou Medical University,Guiyang 550001,China；2.Department of Endocrinology,Guiqian International General Hospital,Guiyang 550018,China)
关键词:
鸢尾素; 白细胞介素-1β; 大鼠甲状腺细胞; 增殖活性; 半胱氨酸天冬氨酸蛋白酶-3; BCL2相关X蛋白; B细胞淋巴瘤-2蛋白; 核因子-κB信号通路
Keywords:
Irisin; Interleukin-1β; Rat thyroid cells; Cell viability; Cleaved Caspase-3; BCL2 associated X protein; B-cell lymphoma 2; Nuclear factor kappa B signaling pathway
分类号:
R 581
DOI:
DOI:10.3969/j.issn.1000-7377.2026.07.002
文献标志码:
A
摘要:
目的：探讨鸢尾素（Irisin）对白细胞介素-1β（IL-1β）诱导大鼠甲状腺细胞（FRTL-5细胞）凋亡的影响及可能的作用机制。方法：将FRTL-5细胞随机分为对照组、IL-1β组、IL-1β+Irisin组、IL-1β+Irisin+BAY11-7028[核因子-κB（NF-κB）信号通路抑制剂]组、IL-1β+BAY11-7028组；通过qPCR测定对照组与IL-1β组细胞中Irisin mRNA的表达水平，采用CCK8法检测各组细胞的增殖活性，Western blot检测各组细胞中半胱氨酸天冬氨酸蛋白酶-3（Caspase-3）、BCL2相关X蛋白（Bax）、B细胞淋巴瘤-2蛋白（Bcl-2）、p65和磷酸化p65（p-p65）的蛋白表达水平。结果：IL-1β刺激FRTL-5细胞后，细胞增殖活性下降，Caspase-3、Bax水平升高，Bcl-2、Bcl-2/Bax表达下降，细胞中Irisin mRNA水平降低（均P＜0.05）。Irisin可提高IL-1β刺激下FRTL-5细胞增殖活性，下调Caspase-3、Bax水平，促进Bcl-2、Bcl-2/Bax表达（均P＜0.05）。Irisin可改善IL-1β刺激下p65的磷酸化，特异性抑制NF-κB（加入BAY11-7082）后，细胞中Bcl-2、Bcl-2/Bax表达下降，Caspase-3、Bax表达上升（均P＜0.05）。结论：Irisin能够改善IL-1β抑制的甲状腺细胞增殖活性，并且缓解IL-1β诱导的甲状腺细胞凋亡，其作用机制可能与Irisin对NF-κB信号通路的调节有关。
Abstract:
Objective:To explore the effect and mechanism of Irisin on the apoptosis of rat thyroid cells (FRTL-5 cells) induced by interleukin-1β (IL-1β).Methods：We established control group,IL-1β group,IL-1β + Irisin group,IL-1β+ Irisin+BAY11-7028 group,IL-1β+BAY11-7028 group.FRTL-5 cells were cultured in vitro.The Irisin mRNA in the cells of the control group and the IL-1β group was detected using qPCR.The cell viability was detected with CCK8 method.The expressions of Cleaved Caspase-3 (Caspase-3),BCL2 associated X protein (Bax),B-cell lymphoma 2 (Bcl-2),p65 and p-p65 proteins in cells were detected with Western blotting.Results:After IL-1β stimulated FRTL-5 cells,the cell viability was decreased,the protein levels of Caspase-3 and Bax were increased,the expression levels of Bcl-2 and the ratio of Bcl-2/Bax were decreased,and the mRNA expression of Irisin was also decreased (all P＜0.05).Irisin can enhance the cell viability of FRTL-5 cells stimulated by IL-1β,down-regulate the levels of Caspase-3 and Bax,and promote the expressions of Bcl-2 and Bcl-2/Bax (allP＜0.05).Irisin declined the IL-1β stimulated phosphorylations of p65.After treatment with the BAY11-7082,the expressions of Bcl-2 and Bcl-2/Bax in cells decreased,while the expressions of Caspase-3 and Bax increased (all P＜0.05).Conclusion:Irisin can enhance the proliferation activity of thyroid cells inhibited by IL-1β,and alleviate the apoptosis of thyroid cells induced by IL-1β.The mechanism of its action may be related to the regulation of NF-κB signaling pathway by Irisin.

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备注/Memo

备注/Memo:
贵州省卫生健康委科学技术基金资助项目（GZWKJ2024-286）；贵州省科技计划项目（黔科合基础-ZK[2024]一般245）
更新日期/Last Update: 2026-07-10