[1]陈攀,张煜,赵启生.麦冬皂苷B调节cGAS-STING信号通路对甲状腺癌TPC-1细胞凋亡、增殖和免疫逃逸的影响[J].陕西医学杂志,2026,(6):769-774.[doi:DOI:10.3969/j.issn.1000-7377.2026.06.008]
　CHEN Pan,ZHANG Yu,ZHAO Qisheng.Effects of OP-B on apoptosis，proliferation and immune escape of thyroid cancer TPC-1 cells by regulating cGAS-STING signaling pathway[J].,2026,(6):769-774.[doi:DOI:10.3969/j.issn.1000-7377.2026.06.008]
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麦冬皂苷B调节cGAS-STING信号通路对甲状腺癌TPC-1细胞凋亡、增殖和免疫逃逸的影响

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2026年6期
页码:
769-774
栏目:
基础研究
出版日期:
2026-06-05

文章信息/Info

Title:
Effects of OP-B on apoptosis，proliferation and immune escape of thyroid cancer TPC-1 cells by regulating cGAS-STING signaling pathway
作者:
陈攀; 张煜; 赵启生
（湖北医药学院附属随州医院 随州市中心医院甲乳外科，湖北 随州 441300）
Author(s):
CHEN Pan; ZHANG Yu; ZHAO Qisheng
(Department of Thyroid and Breast Surgery，Suizhou Hospital Affiliated to Hubei University of Medicine，Suizhou 441300，China)
关键词:
甲状腺癌; 麦冬皂苷B; 增殖; 凋亡; 免疫逃逸; 环状GMP-AMP合成酶-干扰素基因刺激蛋白信号通路
Keywords:
Thyroid cancer; Ophiopogonin B; Proliferation; Apoptosis; Immune escape; Cyclic GMP-AMP synthase-stimulator of interferon genes signaling pathway
分类号:
R -33
DOI:
DOI:10.3969/j.issn.1000-7377.2026.06.008
文献标志码:
A
摘要:
目的：探讨麦冬皂苷B（OP-B）对甲状腺癌TPC-1细胞凋亡、增殖和免疫逃逸的影响及其与环状GMP-AMP合成酶（cGAS）-干扰素基因刺激蛋白（STING）信号通路的调节机制。方法：将TPC-1细胞分为TPC-1组（未做任何处理的TPC-1细胞）、L-OP-B组(2.5 μmol/L OP-B处理TPC-1细胞)、M-OP-B组(5 μmol/L OP-B处理TPC-1细胞)、H-OP-B组(10 μmol/L OP-B处理TPC-1细胞)、RU.521组(100μmol/L cGAS-STING信号通路抑制剂RU.521处理TPC-1细胞)、H-OP-B＋RU.521组（100 μmol/L RU.521和10 μmol/L OP-B共同处理TPC-1细胞）。MTT法检测OP-B对正常人甲状腺细胞系NTHY ORI 3-1细胞和TPC-1细胞的毒性；MTT法检测TPC-1细胞增殖；流式细胞术检测TPC-1细胞凋亡；将CD8+ T细胞与各组处理的TPC-1细胞共培养，台盼蓝染色及流式细胞术检测CD8+ T细胞活力；酶联免疫吸附（ELISA）检测上清液中γ干扰素(IFN-γ)、肿瘤坏死因子-α(TNF-α)水平；Western blot检测程序性死亡配体1（PD-L1）、cGAS-STING通路蛋白水平。结果：与TPC-1组比较，L-OP-B组、M-OP-B组、H-OP-B组OD值和PD-L1蛋白水平显著降低，CD8+ T细胞比例、CD8+ T细胞存活率、TPC-1凋亡率、IFN-γ和TNF-α水平、cGAS和STING蛋白水平显著升高，且OP-B的作用效果呈现剂量相关性，而RU.521组以上指标呈现相反的趋势（均P<0.05）。与H-OP-B组比较，H-OP-B＋RU.521组OD值、PD-L1蛋白水平显著升高，TPC-1凋亡率、CD8+ T细胞比例、CD8+ T细胞存活率、IFN-γ和TNF-α水平、cGAS和STING蛋白水平显著下降（均P<0.05）。结论：OP-B可诱导TPC-1细胞凋亡，抑制细胞增殖和免疫逃逸，其机制可能与激活cGAS-STING信号通路相关。
Abstract:
Objective:To investigate the effect of Ophiopogonin B (OP-B) on the apoptosis，proliferation and immune escape of thyroid cancer TPC-1 cells and the regulatory mechanism of cyclic GMP-AMP synthase (cGAS)-stimulator of interferon gene (STING) signaling pathway.Methods：TPC-1 cells were grouped into TPC-1 group (untreated TPC-1 cells)，L-OP-B group (2.5 μmol/L OP-B treated TPC-1 cells)，M-OP-B group (5 μmol/L OP-B treated TPC-1 cells)，H-OP-B group (10 μmol/L OP-B treated TPC-1 cells)，RU.521 group (100 μmol/L cGAS-STING signaling pathway inhibitor RU.521 treated TPC-1 cells)，and H-OP-B+RU.521 group (100 μmol/L RU.521 and 10 μmol/L OP-B co-treated TPC-1 cells).MTT method was applied to detect the toxicity of OP-B on normal human thyroid cell line NTHY ORI 3-1 cells and TPC-1 cells.MTT method was applied to detect TPC-1 cell proliferation.Flow cytometry was applied to detect apoptosis of TPC-1 cells.CD8+ T cells were co-cultured with TPC-1 cells in each group，detection of CD8+ T cell viability was performed by trypan blue staining and flow cytometry.ELISA method was applied to detect the levels of IFN-γ and TNF-α in the supernatant.Western blot was applied to detect programmed death ligand 1 (PD-L1) and cGAS-STING pathway protein levels.Results:Compared with the TPC-1 group，the OD value and PD-L1 protein level in the L-OP-B group，M-OP-B group，and H-OP-B group were obviously reduced，the apoptosis rate of TPC-1 cells，proportion of CD8+ T cells，survival rate of CD8+ T cells，the IFN-γ and TNF-α levels，cGAS and STING protein levels were obviously increased，and the effect of OP-B showed a dose-dependent relationship，while the RU.521 group showed an opposite trend in the above indicators (all P<0.05).Compared with the H-OP-B group，the OD value and protein level of PD-L1 in the H-OP-B+RU.521 group were obviously increased，the apoptosis rate of TPC-1 cells，proportion of CD8+T cells，survival rate of CD8+ T cells，the IFN-γ and TNF-α levels，cGAS and STING protein levels were obviously reduced (all P<0.05).Conclusion:OP-B can induce apoptosis of TPC-1 cells，inhibit cell proliferation and immune escape，and the mechanism may be related to the activation of cGAS-STING signaling pathway.

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备注/Memo

备注/Memo:
湖北省卫生健康科研指导性项目（WJ2021F087）
更新日期/Last Update: 2026-06-05