[1]艾雪,毕秋英,张新红,等.右美托咪定调控Cav-1/NF-κB信号通路对子宫内膜癌细胞增殖、迁移和侵袭的影响实验研究[J].陕西医学杂志,2026,(2):153-158.[doi:DOI:10.3969/j.issn.1000-7377.2026.02.002]
　AI Xue,BI Qiuying,ZHANG Xinhong,et al.Effects of dexmedetomidine on proliferation,migration and invasion of endometrial cancer cells by regulating the Cav-1/NF-κB signaling pathway[J].,2026,(2):153-158.[doi:DOI:10.3969/j.issn.1000-7377.2026.02.002]
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右美托咪定调控Cav-1/NF-κB信号通路对子宫内膜癌细胞增殖、迁移和侵袭的影响实验研究

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2026年2期
页码:
153-158
栏目:
基础研究
出版日期:
2026-02-05

文章信息/Info

Title:
Effects of dexmedetomidine on proliferation,migration and invasion of endometrial cancer cells by regulating the Cav-1/NF-κB signaling pathway
作者:
艾雪1; 毕秋英2; 张新红3; 任少辉2; 赵贝贝2; 崔杨4; NELSON Eleanor4（英国）
（1.北京中医药大学东方医院秦皇岛医院 秦皇岛市中医医院麻醉科，河北 秦皇岛 066000；2.邢台市人民医院妇科，河北 邢台 054001；3.邢台市第九医院妇产科，河北 邢台 055250；4.邢台医学院第二附属医院检验科，河北 邢台 054000）
Author(s):
AI Xue1; BI Qiuying2; ZHANG Xinhong3; REN Shaohui2; ZHAO Beibei2; CUI Yang4; NELSON Eleanor4
(1.Department of Anesthesiology,Qinhuangdao Hospital,Dongfang Hospital,Beijing University of Chinese Medicine,Qinhuangdao 066000,China;2.Department of Gynecology,Xingtai People’s Hospital,Xingtai 054001,China;3.Department of Obstetrics and Gynecology,Xingtai Ninth Hospital,Xingtai 055250,China;4.Department of Clinical Laboratory,Second Affiliated Hospital of Xingtai Medical College,Xingtai 054000,China)
关键词:
子宫内膜癌; 右美托咪定; 小窝蛋白-1/核因子-κB信号通路; 增殖; 侵袭; 迁移; 移植瘤
Keywords:
Endometrial cancer; Dexmedetomidine; Caveolin-1/nuclear factor-κB signaling pathway; Proliferation; Invasion; Migration; Xenograft tumor
分类号:
R 36
DOI:
DOI:10.3969/j.issn.1000-7377.2026.02.002
文献标志码:
A
摘要:
目的：探讨右美托咪定（Dex）调控小窝蛋白-1（Cav-1）/核因子-κB（NF-κB）信号通路对子宫内膜癌细胞增殖、迁移和侵袭的影响。方法：选取人子宫内膜癌HEC-1A细胞，分为对照组（常规培养，无干预）、Dex低剂量组（加入10 nmol/L Dex）、Dex中剂量组（加入20 nmol/L Dex）、Dex高剂量组（加入40 nmol/L Dex）、Dex高剂量+NF-κB激活剂佛波酯（PMA）组（加入40 nmol/L Dex+1 μmol/L PMA）。CCK-8法检测各组HEC-1A细胞增殖能力，Transwell实验、细胞划痕实验检测各组细胞侵袭和迁移能力，RT-qPCR、Western blot检测各组HEC-1A细胞中Cav-1、NF-κB p65 mRNA和蛋白表达。建立裸鼠皮下移植瘤模型，分组与干预同细胞实验，每组10只。比较各组裸鼠皮下移植瘤体积和重量。结果：24、48 h各组HEC-1A细胞增殖率比较，Dex低、中、高剂量组依次低于对照组，Dex高剂量+PMA组高于Dex高剂量组（均P＜0.05）。各组HEC-1A细胞划痕愈合率、细胞侵袭数目比较，Dex低、中、高剂量组依次低于对照组，Dex高剂量+PMA组高于Dex高剂量组（均P＜0.05）。各组HEC-1A细胞中Cav-1 mRNA及蛋白表达水平比较，Dex低、中、高剂量组依次高于对照组，Dex高剂量+PMA组低于Dex高剂量组（均P＜0.05）。各组HEC-1A细胞中NF-κB p65 mRNA及蛋白表达水平比较，Dex低、中、高剂量组依次低于对照组，Dex高剂量+PMA组高于Dex高剂量组（均P＜0.05）。各组裸鼠皮下移植瘤体积和重量比较，Dex低、中、高剂量组依次低于对照组，Dex高剂量+PMA组高于Dex高剂量组（均P＜0.05）。结论：右美托咪定能够抑制子宫内膜癌细胞增殖、侵袭、迁移以及裸鼠皮下移植瘤生长，其作用机制可能与调控Cav-1/NF-κB信号通路有关。
Abstract:
Objective:To investigate the effects of dexmedetomidine (Dex) on the proliferation,migration,and invasion of endometrial cancer cells by regulating the caveolin-1 (Cav-1)/nuclear factor-κB (NF-κB) signaling pathway.Methods：Human endometrial cancer HEC-1A cells were divided into the following groups：control group (routine culture without intervention),low-dose Dex group (treated with 10 nmol/L Dex),medium-dose Dex group (treated with 20 nmol/L Dex),high-dose Dex group (treated with 40 nmol/L Dex),and high-dose Dex+NF-κB activator PMA group (treated with 40 nmol/L Dex+1 μmol/L PMA).Cell proliferation was assessed using the CCK-8 assay.Cell invasion and migration were evaluated using Transwell and wound healing assays.RT-qPCR and Western blot were used to detect the mRNA and protein expression levels of Cav-1 and NF-κB p65 in HEC-1A cells.A subcutaneous xenograft tumor model in nude mice was established.The grouping and intervention methods were consistent with those in the cell experiment,with 10 mice in each group.Tumor volume and weight were compared among the groups.Results:At 24 and 48 hours,cell proliferation rates in the low-,medium-,and high-dose Dex groups were sequentially lower than in the control group,while the Dex+PMA group showed higher proliferation than the high-dose Dex group (all P<0.05).Wound healing rates and the number of invasive cells were sequentially lower in the Dex-treated groups compared to the control group,with the Dex+PMA group showing increased migration and invasion compared to the high-dose Dex group (all P<0.05).Cav-1 mRNA and protein expression levels were sequentially higher in the Dex-treated groups than in the control group,while the Dex+PMA group showed reduced Cav-1 expression compared to the high-dose Dex group (all P<0.05).NF-κB p65 mRNA and protein expression levels were sequentially lower in the Dex-treated groups than in the control group,while the Dex+PMA group exhibited increased NF-κB p65 expression compared to the high-dose Dex group (all P<0.05).Tumor volume and weight in the nude mice were sequentially lower in the Dex-treated groups compared to the control group,while the Dex+PMA group showed increased tumor growth compared to the high-dose Dex group (all P<0.05).Conclusion:Dexmedetomidine can inhibit the proliferation,invasion and migration of endometrial cancer cells as well as the growth of subcutaneous xenograft tumors in nude mice,and its mechanism may be related to the regulation of the Cav-1/NF-κB signaling pathway.

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备注/Memo

备注/Memo:
国家自然科学基金资助项目（81603430）；河北省中医药管理局科研计划项目（2020549）
更新日期/Last Update: 2026-02-04