[1]夏婧,陈伟特.基于SCF/C-kit信号通路探讨川陈皮素对慢性传输型便秘大鼠肠道蠕动的改善作用实验研究[J].陕西医学杂志,2025,54(12):1616-1621.[doi:DOI:10.3969/j.issn.1000-7377.2025.12.005]
　XIA Jing,CHEN Weite.Improvement effect of nobiletin on intestinal peristalsis in rats with chronic transit constipation based on SCF/C-kit signaling pathway[J].,2025,54(12):1616-1621.[doi:DOI:10.3969/j.issn.1000-7377.2025.12.005]
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基于SCF/C-kit信号通路探讨川陈皮素对慢性传输型便秘大鼠肠道蠕动的改善作用实验研究

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
54
期数:
2025年12期
页码:
1616-1621
栏目:
基础研究
出版日期:
2025-12-05

文章信息/Info

Title:
Improvement effect of nobiletin on intestinal peristalsis in rats with chronic transit constipation based on SCF/C-kit signaling pathway
作者:
夏婧; 陈伟特
（中国人民解放军联勤保障部队第九〇〇医院中医科，福建 福州 350025）
Author(s):
XIA Jing; CHEN Weite
(Department of Traditional Chinese Medicine,900th Hospital,Joint Logistics Support Force of PLA,Fuzhou 350025,China)
关键词:
慢性传输型便秘; 川陈皮素; 干细胞因子/酪氨酸激酶受体信号通路; 肠道蠕动; 大鼠
Keywords:
Slow transit constipation; Nobiletin; SCF/C-kit signaling pathway; Intestinal peristalsis; Rats
分类号:
R 285.5
DOI:
DOI:10.3969/j.issn.1000-7377.2025.12.005
文献标志码:
A
摘要:
目的：基于干细胞因子（SCF）/酪氨酸激酶受体（C-kit）信号通路探讨川陈皮素对慢性传输型便秘（STC）大鼠肠道蠕动的改善作用。方法：选取SPF级SD大鼠40只，随机选取其中10只为空白组，其余建立STC模型，将建模成功的27只分为模型组、川陈皮素组及川陈皮素＋ISCK03（SCF/C-kit通路抑制剂）组，每组各9只。比较各组血清学指标、病理形态变化、小肠推进率、粪便含水率、粪便排出量、胃排空率、炭墨推进情况以及SCF、C-kit mRNA和蛋白表达。结果：与空白组比较，模型组、川陈皮素组和川陈皮素+ISCK03组胃排空率、小肠推进率、粪便含水率、粪便排出量、炭墨推进率、炭墨推进长度、P-物质（SP）、5-羟色胺（5-HT）水平以及SCF、C-kit mRNA和蛋白表达下降，一氧化氮合成酶（NOS）、血管活性肠肽（VIP）、白细胞介素-6（IL-6）、IL-1β、肿瘤坏死因子-α（TNF-α）水平升高（均P<0.05）。与模型组比较，川陈皮素组胃排空率、小肠推进率、粪便含水率、粪便排出量、炭墨推进率、炭墨推进长度、SP、5-HT水平以及SCF、C-kit mRNA和蛋白表达升高，NOS、VIP、IL-6、IL-1β、TNF-α水平下降（均P<0.05）。与川陈皮素比较，川陈皮素＋ISCK03组胃排空率、小肠推进率、炭墨推进率、炭墨推进长度、粪便含水率、粪便排出量、SP、5-HT水平以及SCF、C-kit mRNA表达和蛋白表达下降，NOS、VIP、IL-6、IL-1β、TNF-α水平升高（均P<0.05）。结论：川陈皮素可能通过调控SCF/C-kit通路调节STC大鼠肠道蠕动，改善便秘症状。
Abstract:
Objective:To investigate the improvement effect of nobiletin on intestinal peristalsis in rats with slow transit constipation (STC) based on the stem cell factor (SCF)/tyrosine kinase receptor (C-kit) signaling pathway.Methods：Forty SPF-grade SD rats were selected as experimental animals,and 10 rats were randomly selected as blank group,and the rest were established as chronic transmission constipation model,and 27 rats with successful modeling were divided into the model group,nobiletin group and nobiletin＋ISCK03 group,with 9 rats in each group.Comparison of serum indexes,pathomorphologic changes,small intestinal propulsion rate,fecal water content,fecal excretion,gastric emptying rate,charcoal ink propulsion rate,and the mRNA and protein expressions of SCF and C-kit were made in each group.Results:Compared with the blank group,gastric emptying rate,small intestinal propulsion rate,fecal water content,fecal excretion,charcoal ink propulsion rate,charcoal ink propulsion length,SP and 5-HT levels,SCF and C-kit mRNA and protein decreased,and the levels of NOS,VIP,IL-6,IL-1β,and TNF-α increased in the model group,nobiletin group,and nobiletin＋ISCK03 group (all P<0.05).Compared with the model group,the small intestinal propulsion rate,fecal water content,fecal excretion,gastric emptying rate,charcoal ink propulsion rate,charcoal ink propulsion length,SP and 5-HT levels,SCF and C-kit mRNA and protein were elevated,and the levels of IL-6,IL-1β,TNF-α,NOS,and VIP were decreased in the nobiletin group (all P<0.05).Compared with the nobiletin group,gastric emptying rate,small intestinal propulsion rate,charcoal ink propulsion rate,charcoal ink propulsion length,fecal water content,fecal excretion,SP and 5-HT levels,SCF and C-kit mRNA and protein were decreased,and the levels of NOS,VIP,IL-6,IL-1β,and TNF-α were elevated in the nobiletin＋ISCK03 group (all P<0.05).Conclusion:Nobiletin may regulate the intestinal peristalsis of STC rats by modulating the SCF/C-kit pathway,thereby alleviating constipation symptoms.

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备注/Memo

备注/Memo:
福建医学科技青年培育项目（22FBQN2022114）
更新日期/Last Update: 2025-12-05