[1]张高飞,吴海林,于娇,等.lncRNA TRHDE-AS1调节miR-340-5p/B3GNT5轴对肝癌细胞增殖、迁移和侵袭的影响实验研究[J].陕西医学杂志,2025,54(12):1601-1607,1615.[doi:DOI:10.3969/j.issn.1000-7377.2025.12.003]
　ZHANG Gaofei,WU Hailin,YU Jiao,et al.Effects of lncRNA TRHDE-AS1 regulating miR-340-5p/B3GNT5 axis on proliferation,migration and invasion of liver cancer cells[J].,2025,54(12):1601-1607,1615.[doi:DOI:10.3969/j.issn.1000-7377.2025.12.003]
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lncRNA TRHDE-AS1调节miR-340-5p/B3GNT5轴对肝癌细胞增殖、迁移和侵袭的影响实验研究

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
54
期数:
2025年12期
页码:
1601-1607,1615
栏目:
基础研究
出版日期:
2025-12-05

文章信息/Info

Title:
Effects of lncRNA TRHDE-AS1 regulating miR-340-5p/B3GNT5 axis on proliferation,migration and invasion of liver cancer cells
作者:
张高飞1; 吴海林2; 于娇1; 赵翊3; 刘宇杰3
（1.陕西省人民医院放疗科，陕西 西安 710068；2.陕西省人民医院医学装备部，陕西 西安 710068；3.陕西省人民医院肿瘤外科，陕西 西安 710068）
Author(s):
ZHANG Gaofei1; WU Hailin2; YU Jiao1; ZHAO Yi3; LIU Yujie3
(1.Department of Radiotherapy,Shaanxi Provincial People’s Hospital,Xi’an 710068,China;2.Department of Medical Equipment,Shaanxi Provincial People’s Hospital,Xi’an 710068,China;3.Department of Oncology,Shaanxi Provincial People’s Hospital,Xi’an 710068,China)
关键词:
肝癌; 长链非编码RNA促甲状腺激素释放激素降解酶反义RNA1; 微小RNA-340-5p; β-1; 3-N-乙酰氨基葡萄糖基转移酶5; 靶向调控; 分子机制
Keywords:
Liver cancer; lncRNA TRHDE-AS1; miR-340-5p; B3GNT5; Targeted regulation; Molecular mechanism
分类号:
R -33
DOI:
DOI:10.3969/j.issn.1000-7377.2025.12.003
文献标志码:
A
摘要:
目的：探讨长链非编码RNA促甲状腺激素释放激素降解酶反义RNA1(lncRNA TRHDE-AS1)调节微小RNA-340-5p（miR-340-5p）/β-1，3-N-乙酰氨基葡萄糖基转移酶5 (B3GNT5)对肝癌细胞增殖、迁移和侵袭的影响。方法：实时荧光定量PCR（RT-qPCR）、Western blot分别检测肝癌组织与癌旁组织及各肝癌细胞（HuH-7、HCCLM3、HepG2）中lncRNA TRHDE-AS1、miR-340-5p表达及B3GNT5蛋白表达。将HepG2细胞分为空白组、si-NC组、si-lncRNA TRHDE-AS1组、miR-NC组、miR-340-5p组、si-lncRNA TRHDE-AS1＋anti-miR-NC组、si-lncRNA TRHDE-AS1＋anti-miR-340-5p 组，采用RT-qPCR检测各组细胞中lncRNA TRHDE-AS1、miR-340-5p表达及增殖细胞核抗原（PCNA）、基质金属蛋白酶（MMP）-9、MMP-2 mRNA表达，采用Western blot检测各组细胞中B3GNT5蛋白表达，采用克隆形成实验、CCK-8实验、划痕愈合实验、Transwell实验检测细胞增殖、迁移、侵袭能力。采用双荧光素酶基因报告基因实验验证lncRNA TRHDE-AS1与miR-340-5p、miR-340-5p与B3GNT5的靶向关系。结果：沉默lncRNA TRHDE-AS1或过表达miR-340-5p均可抑制HepG2细胞增殖、迁移与侵袭。下调miR-340-5p减弱了沉默lncRNA TRHDE-AS1对HepG2细胞恶性行为的抑制作用。lncRNA TRHDE-AS1靶向调控miR-340-5p/B3GNT5轴。结论：沉默lncRNA TRHDE-AS1可能通过上调miR-340-5p并抑制B3GNT5表达来抑制HepG2细胞增殖、迁移与侵袭。
Abstract:
Objective:To investigate the effects of long non-coding thyrotropin releasing hormone degrading enzyme antisense RNA1 (lncRNA TRHDE-AS1) on migration,proliferation and invasion of liver cancer cells by regulating miR-340-5p/beta-1,3-N-acetylglucosaminyltransferase 5 (B3GNT5).Methods：The expression of lncRNA TRHDE-AS1,miR-340-5p and B3GNT5 protein in liver cancer tissues,adjacent tissues and various liver cancer cells (HuH-7,HCCLM3,HepG2) was detected by RT-qPCR and Western blot.HepG2 cells were separated into blank group,si-NC group,si-lncRNA TRHDE-AS1 group,miR-NC group,miR-340-5p group,si-lncRNA TRHDE-AS1＋anti miR-NC group,and si-lncRNA TRHDE-AS1＋anti miR-340-5p group.RT-qPCR was applied to detect the expression of lncRNA TRHDE-AS1,miR-340-5p,PCNA,MMP-9,and MMP-2 mRNA.Western blot was applied to detect the expression of B3GNT5 protein.Clone formation experiment,CCK-8 experiment,scratch healing experiment,and Transwell experiment were applied to detect cell proliferation,migration,and invasion,respectively.The targeting relationship between lncRNA TRHDE-AS1 and miR-340-5p,as well as between miR-340-5p and B3GNT5 was verified by using the dual luciferase gene reporter assay.Results:Silencing lncRNA TRHDE-AS1 or overexpressing miR-340-5p was able to inhibit HepG2 cell proliferation,migration,and invasion.Down-regulation of miR-340-5p weakened the inhibitory effect of silenced lncRNA TRHDE-AS1 on malignant behavior of HepG2 cells.LncRNA TRHDE-AS1 targeted the regulation of miR-340-5p/B3GNT5 axis.Conclusion:Silencing lncRNA TRHDE-AS1 can inhibit the proliferation,migration and invasion of HepG2 cells.Its molecular mechanism may be related to the upregulation of miR-340-5p and the inhibition of B3GNT5 expression.

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备注/Memo

备注/Memo:
陕西省重点研发计划项目（2023-YBSF-329）；陕西省人民医院科技人才支持计划项目（2022JY-08）；陕西省人民医院科技发展孵化基金资助项目（2023YJY-49）
更新日期/Last Update: 2025-12-05