[1]向文远,邓迎杰,梁治权,等.TRPM7/CaMKⅡ/SMAD1信号通路在膝骨关节炎软骨组织损伤中的作用实验研究[J].陕西医学杂志,2025,54(12):1596-1600,1634.[doi:DOI:10.3969/j.issn.1000-7377.2025.12.002]
　XIANG Wenyuan,DENG Yingjie,LIANG Zhiquan,et al.Role of TRPM7/CaMKⅡ/SMAD1 signaling pathway in cartilage tissue injury of knee osteoarthritis[J].,2025,54(12):1596-1600,1634.[doi:DOI:10.3969/j.issn.1000-7377.2025.12.002]
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TRPM7/CaMKⅡ/SMAD1信号通路在膝骨关节炎软骨组织损伤中的作用实验研究

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
54
期数:
2025年12期
页码:
1596-1600,1634
栏目:
基础研究
出版日期:
2025-12-05

文章信息/Info

Title:
Role of TRPM7/CaMKⅡ/SMAD1 signaling pathway in cartilage tissue injury of knee osteoarthritis
作者:
向文远; 邓迎杰; 梁治权; 张文豪; 方锐
（新疆医科大学第四附属医院关节外科，新疆 乌鲁木齐 830000）
Author(s):
XIANG Wenyuan; DENG Yingjie; LIANG Zhiquan; ZHANG Wenhao; FANG Rui
(Department of Joint Surgery,the Fourth Affiliated Hospital of Xinjiang Medical University,Urumqi 830000,China)
关键词:
膝骨关节炎; 软骨组织; 瞬时受体电位离子通道蛋白7; 钙调素依赖性蛋白激酶Ⅱ; 炎症因子; 氧化应激; 钙离子; 信号通路
Keywords:
Knee osteoarthritis; Cartilage tissue; Transient receptor potential melastatin 7; Calmodulin-dependent protein kinase Ⅱ; Inflammatory factors; Oxidative stress; Calcium ion; Signaling pathway
分类号:
R -33
DOI:
DOI:10.3969/j.issn.1000-7377.2025.12.002
文献标志码:
A
摘要:
目的：探讨瞬时受体电位离子通道蛋白7（TRPM7）/钙调素依赖性蛋白激酶Ⅱ（CaMKⅡ）/SMAD1信号通路在膝骨关节炎（KOA）软骨组织损伤中的作用，为治疗KOA提供新参考。方法：收集需要手术的 KOA患者（KOA组）和创伤无KOA的患者（非KOA组）各15例为研究对象，制备关节软骨组织。酶联免疫吸附法（ELISA）检测关节软骨组织炎症因子一氧化氮合酶（iNOS）、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）水平。流式细胞术测定关节软骨组织活性氧（ROS）和Ca2+水平。实时荧光定量PCR（RT-qPCR）检测关节软骨组织TRPM7基因表达。Western blot检测关节软骨组织TRPM7/CaMKⅡ/SMAD1信号通路相关蛋白表达。结果：与非KOA组比较，KOA组关节软骨组织iNOS、TNF-α、IL-6、ROS、Ca2+水平升高，TRPM7基因及TRPM7、p-CaMKⅡ、p-SMAD1蛋白呈高表达(均P<0.05)。结论：KOA患者软骨组织处于炎症状态和高氧化应激状态。TRPM7激活触发Ca2+内流，激活下游靶蛋白CaMKⅡ和SMAD1，进而调节软骨细胞活性。TRPM7/CaMKⅡ/SMAD1信号通路可能是KOA的发病机制之一。
Abstract:
Objective:To investigate the role of transient receptor potential melastatin 7 (TRPM7)/calmodulin-dependent protein kinase Ⅱ (CaMKⅡ)/SMAD1 signaling pathway in cartilage tissue injury in knee osteoarthritis (KOA),and to provide a new reference for the treatment of KOA.Methods：Fifteen patients with KOA who needed surgery (KOA group) and 15 trauma patients without KOA (non-KOA group) were collected as research objects,and articular cartilage tissue was prepared.In articular cartilage tissue,ELISA was used to detect the levels of inflammatory factors iNOS,TNF-α and IL-6,Flow cytometry was used to measure ROS and Ca2+ levels,RT-qPCR was used to detect the expression of TRPM7 gene,and Western blot was used to detect the expression of TRPM7/CaMKⅡ/SMAD1 signaling pathway related proteins.Results:Compared with the non-KOA group,the levels of iNOS,TNF-α,IL-6,ROS and Ca2+ in articular cartilage tissue were increased,and the expressions of TRPM7 gene and TRPM7,p-CaMKⅡ and p-SMAD1 proteins were increased in the KOA group (all P<0.05).Conclusion:The cartilage tissue of KOA patients is in a state of inflammation and high oxidative stress.TRPM7 activation triggers Ca2+ influx and activates the downstream target proteins CaMKⅡ and SMAD1,thereby regulating chondrocyte activity.TRPM7/CaMKⅡ/SMAD1 signaling pathway may be one of the pathogenesis of KOA.

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备注/Memo

备注/Memo:
国家自然科学基金资助项目（82405423）
更新日期/Last Update: 2025-12-05