[1]房冰莹,张鑫强,梁静,等.高盐饮食对大鼠心脏功能及其神经调控的影响及机制实验研究[J].陕西医学杂志,2025,54(10):1305-1310.[doi:DOI:10.3969/j.issn.1000-7377.2025.10.002]
　FANG Bingying,ZHANG Xinqiang,LIANG Jing,et al.The influence and mechanism of high-salt diet on cardiac function and neural regulation in rats[J].,2025,54(10):1305-1310.[doi:DOI:10.3969/j.issn.1000-7377.2025.10.002]
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高盐饮食对大鼠心脏功能及其神经调控的影响及机制实验研究

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
54
期数:
2025年10期
页码:
1305-1310
栏目:
基础研究
出版日期:
2025-10-05

文章信息/Info

Title:
The influence and mechanism of high-salt diet on cardiac function and neural regulation in rats
作者:
房冰莹1; 2; 张鑫强2; 梁静2; 杜婷2; 张琪1; 王海芳2; 刘晓华1; 2; 4
（1.陕西中医药大学基础医学院生理学教研室，陕西 咸阳 712046；2.陕西省中西医结合防治心血管疾病重点实验室，陕西 咸阳 712046；3.陕西中医体质与疾病防治重点实验室，陕西 咸阳 712046）
Author(s):
FANG Bingying1; 2; ZHANG Xinqiang2; LIANG Jing2; DU Ting2; ZHANG Qi1; WANG Haifang2; LIU Xiaohua1; 2; 3
(1.Department of Physiology,School of Basic Medical Sciences,Shaanxi University of Traditional Chinese Medicine,Xianyang 712046,China;2.Shaanxi Key Laboratory of Integrated Traditional and Western Medicine for Prevention and Treatment of Cardiovascular Diseases,Xianyang 712046,China;3.Shaanxi Key Laboratory of Constitution and Disease Prevention of Traditional Chinese Medicine,Xianyang 712046,China)
关键词:
高盐饮食; 心迷走神经反射; 心脏功能; 动脉血压; 心肌损伤; 炎症反应
Keywords:
High-salt diet; Cardiac vagus nerve reflex; Cardiac function; Arterial blood pressure; Myocardial injury; Inflammatory response
分类号:
R 541
DOI:
DOI:10.3969/j.issn.1000-7377.2025.10.002
文献标志码:
A
摘要:
目的：研究高盐饮食对大鼠动脉血压、左心室功能、心迷走神经反射以及心肌损伤和炎症因子表达的影响，以阐明高盐饮食对心脏生理功能的综合影响及其机制。方法：SD大鼠随机分为对照组和高盐组，对照组大鼠喂食正常饲料，高盐组大鼠喂食高盐饲料，均持续8周。分别于实验第0、1、3、5、7周利用尾动脉无创血压测量技术监测大鼠动脉血压，第8周动物麻醉后，经右颈总动脉左心室插管检测左心室血流动力学，心外膜表面滴注辣椒素观察心迷走神经反射对血压和左心室内压的影响，肢体导联记录心电图，ELISA法检测血清肌红蛋白（MYO）、超敏心肌肌钙蛋白（hs-cTn）含量，qRT-PCR法检测心脏心房钠尿肽（ANP）、肌球蛋白重链6（MYH6）、肌球蛋白重链7（MYH7）以及炎症因子的表达。结果：与对照组比较，高盐组大鼠高盐饮食1周后收缩压（SBP）、舒张压（DBP）、平均动脉压（MAP）均开始持续升高（均P<0.05），8周后左心室舒张末期压力（LVEDP）明显升高，左心室内压最大上升速率（dP/dtmax）和最大下降速率（-dP/dtmax）绝对值降低（均P<0.01）；高盐组大鼠心外膜表面滴注辣椒素后SBP、MAP、LVDEP和左心室收缩压（LVSP）的变化率均明显低于对照组（均P<0.05）；同时，高盐组大鼠心脏出现ST段抬高、宽大畸形的QRS波以及房性早搏伴房室传导阻滞等心电异常的变化；且高盐组大鼠血清MYO、hs-cTn浓度显著升高(P<0.05)，心脏ANP、MYH7和白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）和环氧合酶2（COX2）的mRNA表达上升，而MYH6的mRNA表达下降（均P<0.05）。结论：高盐摄入导致大鼠心肌收缩和舒张功能障碍，同时心迷走神经传出敏感性下降，其机制可能与心肌炎症损伤有关。
Abstract:
Objective:To investigate the effects of a high-salt diet on arterial blood pressure,left ventricular function,cardiac vagal nerve reflex,myocardial injury and the expression of inflammatory factors in rats,and to clarify the comprehensive impact of high-salt diet on cardiac physiological function and its mechanism.Methods：SD rats were randomly divided into a control group and a high-salt group.Rats in the control group were fed a normal diet,while those in the high-salt group were fed high-salt diet for a duration of 8 weeks.Arterial blood pressure of rats was monitored at weeks 0,1,3,5 and 7 using the non-invasive tail artery blood pressure measurement technique.At the end of the 8th week,following anesthesia,left ventricular hemodynamics were assessed via left ventricular intubation through the right common carotid artery.The effects of the cardiac vagus nerve reflex on blood pressure and left ventricular pressure were observed after applying capsaicin to the myocardial outer membrane.Electrocardiograms were recorded using limb leads.Serum myoglobin (MYO) and hypersensitive cardiac troponin (hs-cTn) levels were measured using ELISA,meanwhile the expression of atrial natriuretic peptide (ANP),myosin heavy chain 6 (MYH6),myosin heavy chain 7 (MYH7) and inflammatory factors in the heart were detected by qRT-PCR.Results:Compared with the control group,the systolic blood pressure (SBP),diastolic blood pressure (DBP) and mean arterial pressure (MAP) of rats in the high-salt group showed a continuous increase starting one week after the high-salt diet (all P<0.05).After 8 weeks,the left ventricular end-diastolic pressure (LVEDP) increased significantly,and the maximum rate of increase (dP/dtmax) and the absolute value of maximum rate of decrease (-dP/dtmax) of left ventricular pressure were notably lower (all P<0.01).The change rates of SBP,MAP,LVDEP and left ventricular systolic blood pressure (LVSP) in the high-salt group rats after capsaicin application were significantly lower than those in the control group (all P<0.05).Concurrently,the high-salt group exhibited abnormal electrocardiogram changes,including ST-segment elevation,wide and malformed QRS waves,and atrial premature beats with atrioventricular block.Additionally,serum concentrations of MYO and hs-cTn increased significantly in the high-salt group(P<0.05).The mRNA expressions of ANP,MYH7,interleukin-1β (IL-1β),interleukin-6 (IL-6) and cyclooxygenase 2 (COX2) in the heart were upregulated,while that of MYH6 decreased (all P<0.05).Conclusion:High salt intake contributes to myocardial contractile and diastolic dysfunction in rats,coupled with reduced efferent sensitivity of the cardiac vagus nerve.This mechanism may be attributed to myocardial inflammatory injury.

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备注/Memo

备注/Memo:
国家自然科学基金资助项目（82004221）；陕西省教育厅专项科研计划项目（24JK0413）；陕西省教育厅重点实验室项目（23JS005）；陕西中医药大学本科生科研青苗计划项目（2023QY02）；秦创原中医药产业创新聚集区项目（L2024-QCY-ZYYJJQ-X03）
更新日期/Last Update: 2025-10-09