[1]白晓蔚,梁媛哲,井娜,等.微小RNA-21对宫颈癌细胞恶性生物学行为的影响及其机制实验研究[J].陕西医学杂志,2025,54(8):1034-1038.[doi:DOI:10.3969/j.issn.1000-7377.2025.08.005]
　BAI Xiaowei,LIANG Yuanzhe,JING Na,et al.Effects of miR-21 on malignant biological behavior of cervical cancer cells and its mechanism[J].,2025,54(8):1034-1038.[doi:DOI:10.3969/j.issn.1000-7377.2025.08.005]
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微小RNA-21对宫颈癌细胞恶性生物学行为的影响及其机制实验研究

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
54
期数:
2025年8期
页码:
1034-1038
栏目:
基础研究
出版日期:
2025-08-05

文章信息/Info

Title:
Effects of miR-21 on malignant biological behavior of cervical cancer cells and its mechanism
作者:
白晓蔚; 梁媛哲; 井娜; 范爱英; 冯晓英; 修晨; 牛翠丽
（邢台医学院附属医院 邢台市中心医院妇科，河北 邢台 054000）
Author(s):
BAI Xiaowei; LIANG Yuanzhe; JING Na; FAN Aiying; FENG Xiaoying; XIU Chen; NIU Cuili
(Department of Gynecology,the Affiliated Hospital of Xingtai Medical College,Xingtai 054000,China)
关键词:
宫颈癌; 微小RNA-21; PI3K/AKT/mTOR信号通路; 细胞凋亡; 增殖; 侵袭; 迁移
Keywords:
Cervical cancer; miR-21; PI3K/AKT/mTOR signaling pathway; Apoptosis; Proliferation; Invasion; Migration
分类号:
R 36
DOI:
DOI:10.3969/j.issn.1000-7377.2025.08.005
文献标志码:
A
摘要:
目的：探讨微小RNA-21（miR-21）对宫颈癌细胞恶性生物学行为的影响及可能的作用机制。方法：将宫颈癌HeLa细胞随机分为对照组（空白未处理）、miR-NC组（转染miR-NC）、miR-21 mimics组（转染miR-21 mimics）、miR-21 inhibitor组（转染miR-21 inhibitor）。采用流式细胞术、CCK-8法、Transwell实验、细胞划痕实验分别检测HeLa细胞凋亡、增殖、侵袭、迁移情况；RT-qPCR检测HeLa细胞中miR-21表达；Western blot检测HeLa细胞中磷脂酰肌醇3-激酶（PI3K）/蛋白激酶B（AKT）/哺乳动物雷帕霉素靶蛋白（mTOR）信号通路相关蛋白表达。结果：与对照组和miR-NC组比较，miR-21 mimics组HeLa细胞凋亡率下降，细胞增殖率、侵袭数量及迁移率升高；miR-21 inhibitor组HeLa细胞凋亡率升高，细胞增殖率、侵袭数量及迁移率降低（均P＜0.05）。对照组和miR-NC组HeLa细胞中miR-21及p-PI3K、p-AKT、p-mTOR蛋白表达水平低于miR-21 mimics组，高于miR-21 inhibitor组（均P＜0.05）。结论：抑制miR-21能够促进宫颈癌细胞凋亡，降低细胞增殖、侵袭及迁移能力，其机制可能与调控PI3K/AKT/mTOR信号通路有关。
Abstract:
Objective:To investigate the effects of microRNA-21 (miR-21) on the malignant biological behavior of cervical cancer cells and its potential mechanism of action.Methods：Cervical cancer HeLa cells were randomly divided into four groups：the control group (untreated),the miR-NC group (transfected with miR-NC),the miR-21 mimics group (transfected with miR-21 mimics),and the miR-21 inhibitor group (transfected with miR-21 inhibitor).Flow cytometry,CCK-8 assay,Transwell assay and cell scratch assay were respectively used to detect apoptosis,proliferation,invasion and migration of HeLa cells.RT-qPCR was used to measure the expression of miR-21 in HeLa cells,while Western blot was used to detect the expression of proteins related to the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) signaling pathway.Results:Compared with the control and miR-NC groups,the miR-21 mimics group exhibited decreased apoptosis rates and increased proliferation rates,invasive numbers,and migration rates of HeLa cells (all P<0.05).Conversely,the miR-21 inhibitor group showed increased apoptosis rates and decreased proliferation rates,invasive numbers,and migration rates (all P<0.05).The expression levels of miR-21 and p-PI3K,p-AKT,p-mTOR proteins in HeLa cells of the control group and miR-NC group were lower than those in the miR-21 mimics group and higher than those in the miR-21 inhibitor group (all P<0.05).Conclusion:Inhibiting miR-21 can promote the apoptosis of cervical cancer cells,reduce the proliferation,invasion and migration abilities of the cells,and the mechanism may be related to the regulation of the PI3K/AKT/mTOR signaling pathway.

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备注/Memo

备注/Memo:
河北省医学科学研究课题（20201564）；河北省邢台市重点研发计划自筹项目（2022ZC148）
更新日期/Last Update: 2025-08-04