[1]解静,汪永新.MSX1基因调控Wnt/β-catenin通路刺激成骨分化和骨形成机制实验研究[J].陕西医学杂志,2025,54(6):754-758.[doi:DOI:10.3969/j.issn.1000-7377.2025.06.006]
　XIE Jing,WANG Yongxin.Mechanism of MSX1 regulating Wnt/β-catenin pathway to stimulate osteogenic differentiation and bone formation[J].,2025,54(6):754-758.[doi:DOI:10.3969/j.issn.1000-7377.2025.06.006]
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MSX1基因调控Wnt/β-catenin通路刺激成骨分化和骨形成机制实验研究

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
54
期数:
2025年6期
页码:
754-758
栏目:
基础研究
出版日期:
2025-06-05

文章信息/Info

Title:
Mechanism of MSX1 regulating Wnt/β-catenin pathway to stimulate osteogenic differentiation and bone formation
作者:
解静; 汪永新
（新疆医科大学第一附属医院，新疆 乌鲁木齐 830054）
Author(s):
XIE Jing; WANG Yongxin
(The First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China)
关键词:
骨质疏松症; 同源盒基因1; MC3T3-E1细胞; 成骨分化; 骨形成; Wnt/β-catenin通路
Keywords:
Osteoporosis; MSX1; MC3T3-E1 cells; Osteogenic differentiation; Bone formation; Wnt/β-catenin pathway
分类号:
R -33
DOI:
DOI:10.3969/j.issn.1000-7377.2025.06.006
文献标志码:
A
摘要:
目的：探究同源盒基因1（MSX1）调控Wnt/β-catenin通路刺激成骨分化和骨形成的机制。方法：对MC3T3-E1细胞使用成骨诱导培养基（OIM）进行成骨培养，诱导分化后分为对照组、小干扰RNA-阴性对照组（sh-NC组）及sh-MSX1组。实时荧光定量PCR（RT-qPCR）检测MSX1及成骨相关基因[胶原蛋白Ⅰ型（Col1A1）、骨桥蛋白（OPN）、碱性磷酸酶（ALP）、骨钙蛋白（OCN）]；流式细胞仪检测细胞凋亡率；茜素红S染色鉴定MC3T3-E1细胞钙化结节；检测ALP活性；Western blot检测Wnt/β-catenin信号通路相关蛋白。进一步对MC3T3-E1细胞使用Wnt/β-catenin通路激动剂SKL2001后进行分组，分别为对照组、激动剂组、sh-MSX1组和sh-MSX1组+激动剂组，RT-qPCR检测成骨相关基因。结果：与诱导分化第0天比较，OIM以时间依赖性的方式升高了MC3T3-E1细胞中MSX1 mRNA的表达。sh-MSX1组细胞凋亡率高于sh-NC组，ALP活性及钙化面积低于sh-NC组（均P<0.05）。sh-MSX1组Col1A1、OPN、ALP及OCN mRNA表达低于sh-NC组，Wnt1、Wnt3a、β-catenin蛋白表达低于sh-NC组（均P<0.05）。与对照组比较，激动剂组成骨相关基因表达升高，sh-MSX1组降低（均P<0.05）。sh-MSX1组+激动剂组成骨相关基因表达高于sh-MSX1组（均P<0.05）。结论：敲低MSX1可促进MC3T3-E1细胞凋亡，并通过抑制Wnt/β-catenin通路激活降低MC3T3-E1细胞成骨分化和骨形成能力。
Abstract:
Objective:To investigate the mechanism of msh homeobox gene 1 (MSX1) regulating Wnt/β-catenin pathway to stimulate osteogenic differentiation and bone formation.Methods：MC3T3-E1 cells were cultured using osteogenic induction medium (OIM) and divided into three groups：control group,small interference RNA-negative control group (sh-NC group) and sh-MSX1 group.MSX1 and osteogenic genes (Col1A1,OPN,ALP,OCN) were detected by RT-qPCR.The apoptosis rate was detected by flow cytometry.Alizarin red S staining was used to detect calcified nodules in MC3T3-E1 cells,and ALP activity was detected.Western blot was used to detect Wnt/β-catenin signaling pathway-related proteins.MC3T3-E1 cells were further divided into control group,agonist group,sh-MSX1 group and sh-MSX1+agonist group after receiving Wnt/β-catenin pathway agonist SKL2001,and bone formation related genes were detected by RT-qPCR.Results:Compared with day 0,OIM increased MSX1 mRNA expression in MC3T3-E1 cells in a time-dependent manner.The apoptosis rate of sh-MSX1 group was significantly higher than that of sh-NC group,and ALP activity and calcified area of sh-MSX1 group were lower than those of sh-NC group (all P＜0.05).The mRNA expressions of Col1A1,OPN,ALP and OCN in sh-MSX1 group were less than those in the sh-NC group,and the protein expressions of Wnt1,Wnt3a and β-catenin in sh-MSX1 group were lower than those in sh-NC group (all P＜0.05).Compared with the control group,the expression of bone-related genes was increased in the agonist group and decreased in the sh-MSX1 group (all P<0.05).The expression of bone-related genes in the sh-MSX1+agonist group was higher than that in the sh-MSX1 group (all P<0.05).Conclusion:Knockdown of MSX1 can promote the apoptosis of MC3T3-E1 cells,and reduce the osteogenic differentiation and bone formation ability of MC3T3-E1 cells by inhibiting the activation of Wnt/β-catenin pathway.

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备注/Memo

备注/Memo:
国家自然科学基金资助项目（81401806）
更新日期/Last Update: 2025-06-04