[1]李 蕾,谢小琴,刘 强,等.狗肝菜多糖通过降低lncRNA DANCR表达对高糖诱导肾小管上皮细胞损伤的保护作用[J].陕西医学杂志,2025,54(4):447-451.[doi:DOI:10.3969/j.issn.1000-7377.2025.04.003]
　LI Lei,XIE Xiaoqin,LIU Qiang,et al.Protective effect of Dicliptera chinensis polysaccharide on high glucose-induced renal tubular epithelial cell injury by down-regulating lncRNA DANCR expression[J].,2025,54(4):447-451.[doi:DOI:10.3969/j.issn.1000-7377.2025.04.003]
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狗肝菜多糖通过降低lncRNA DANCR表达对高糖诱导肾小管上皮细胞损伤的保护作用
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
54
期数:
2025年4期
页码:
447-451
栏目:
基础研究
出版日期:
2025-04-05

文章信息/Info

Title:
Protective effect of Dicliptera chinensis polysaccharide on high glucose-induced renal tubular epithelial cell injury by down-regulating lncRNA DANCR expression
作者:
李 蕾1; 谢小琴1; 刘 强2; 赵 敏2
(1.成都中医药大学附属医院德阳医院肾内科,四川 德阳 618000; 2.南京大学鼓楼医院肾内科,江苏 南京 210009)
Author(s):
LI Lei1; XIE Xiaoqin1; LIU Qiang2; ZHAO Min2
(1.Department of Nephrology,Deyang Hospital,Affiliated Hospital of Chengdu University of Traditional Chinese Medicine,Deyang 618000,China; 2.Department of Nephrology,Drum Tower Hospital of Nanjing University,Nanjing 210009,China)
关键词:
狗肝菜多糖;  糖尿病肾病;  长链非编码RNA;  微小RNA;  细胞增殖;  细胞凋亡
Keywords:
Dicliptera chinensis polysaccharide;  Diabetic nephropathy;  Long noncoding RNA;  MicroRNA;  Cell proliferation;  Cell apoptosis
分类号:
R 587.6
DOI:
DOI:10.3969/j.issn.1000-7377.2025.04.003
文献标志码:
A
摘要:
目的:探讨狗肝菜多糖对高糖诱导肾小管上皮细胞损伤的拮抗作用及作用机制。方法:选用肾小管上皮细胞系HK-2细胞,以30 mmol/L高糖处理建立HK-2细胞损伤模型。分别添加0、20、40、60、80 μmol/L狗肝菜多糖处理HK-2细胞。采用噻唑蓝(MTT)法检测HK-2细胞的增殖,筛选狗肝菜多糖的最适浓度。0 μmol/L狗肝菜多糖处理的HK-2细胞定为对照组,60 μmol/L狗肝菜多糖处理的HK-2细胞定为狗肝菜多糖组。采用流式细胞术分别检测HK-2细胞的凋亡水平。实时定量聚合酶链反应(qRT-PCR)检测长链非编码RNA(lncRNA)DANCR和微小RNA-214-5p(miR-214-5p)的表达。蛋白质印迹法(Western blotting)检测HK-2细胞中凋亡相关蛋白的表达。结果:与对照组比较,不同浓度的狗肝菜多糖均可显著促进HK-2细胞的增殖活力(均P<0.01),60 μmol/L狗肝菜多糖促进细胞活力最明显(P<0.01)。对照组和狗肝菜多糖组HK-2细胞凋亡率分别为(6.68±0.94)%和(0.54±0.25)%,狗肝菜多糖显著抑制HK-2细胞的凋亡(P<0.01)。与低糖组比较,高糖组HK-2细胞中lncRNA DANCR表达明显升高(P<0.01),miR-214-5p表达明显降低(P<0.01)。与对照组比较,狗肝菜多糖组HK-2细胞中lncRNA DANCR表达明显降低(P<0.01),miR-214-5p表达明显升高(P<0.01)。与对照组比较,狗肝菜多糖组中促凋亡蛋白Bcl-2相关X蛋白(Bax)、Bcl-2同源拮抗剂(Bak)表达降低,抑制凋亡蛋白B细胞淋巴瘤/白血病-2蛋白(Bcl-2)、B细胞淋巴瘤超大蛋白(Bcl-XL)表达升高。结论:狗肝菜多糖通过调控lncRNA DANCR/miR-214-5p分子轴促进肾小管上皮HK-2细胞的增殖并抑制细胞的凋亡。
Abstract:
Objective:To investigate the antagonistic effect and mechanism of Dicliptera chinensis polysaccharide on high glucose-induced renal tubular epithelial cell injury.Methods:HK-2 cells,a renal tubular epithelial cell line,were selected and treated with 30 mmol/L high glucose to establish the HK-2 cell injury model.HK-2 cells were treated with 0,20,40,60,and 80 μmol/L of Dicliptera chinensis polysaccharide,respectively.The proliferation of HK-2 cells was detected by Thiazolyl blue(MTT)method,and the optimal concentration of Dicliptera chinensis polysaccharide was screened.HK-2 cells treated with 0 μmol/L Dicliptera chinensis polysaccharide were designated as the control group,and HK-2 cells treated with 60 μmol/L Dicliptera chinensis polysaccharide were designated as the Dicliptera chinensis polysaccharide group.Flow cytometry was used to detect the apoptosis level of HK-2 cells.Real-time quantitative polymerase chain reaction(qRT-PCR)was used to detect the expression of long non-coding RNA(lncRNA)DANCR and miR-214-5p.Western blotting was used to detect the expression of apoptosis-related proteins in HK-2 cells.Results:Compared with the control group,different concentrations of Dicliptera chinensis polysaccharide could significantly promote the proliferation activity of HK-2 cells(all P<0.01),and 60 μmol/L Dicliptera chinensis polysaccharide promoted cell activity most significantly(P<0.01).The apoptosis rates of HK-2 cells in the control group and Dicliptera chinensis polysaccharide group were(6.68±0.94)% and(0.54±0.25)%,respectively.Cyperus polysaccharide significantly inhibited the apoptosis of HK-2 cells(P<0.01).Compared with the low glucose group,the expression of lncRNA DANCR in HK-2 cells in the high glucose group was significantly increased(P<0.01),and the expression of miR-214-5p was significantly decreased(P<0.01).Compared with the control group,the expression of lncRNA DANCR in HK-2 cells in the Dicliptera chinensis polysaccharide group was significantly decreased(P<0.01),and the expression of miR-214-5p was significantly increased(P<0.01).Compared with the control group,the expressions of pro-apoptotic proteins Bcl-2-associated X protein(Bax)and Bcl-2 homologous antagonist(Bak)in the Dicliptera chinensis polysaccharide group were decreased,and the expressions of anti-apoptotic proteins B-cell lymphoma/leukemia-2(Bcl-2)and B-cell lymphoma-extra-large(Bcl-XL)were increased.Conclusion:Dicliptera chinensis polysaccharide promotes the proliferation of renal tubular epithelial HK-2 cells and inhibits cell apoptosis by regulating the lncRNA DANCR/miR-214-5p molecular axis.

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备注/Memo

备注/Memo:
[基金项目]国家自然科学基金资助项目(81900667)
更新日期/Last Update: 2025-04-07