[1]贺静雯,李得加,张 毅,等.富血小板血浆微球对宫颈癌根治术后尿失禁大鼠尿动力学及阴道前壁胶原代谢影响[J].陕西医学杂志,2025,54(1):38-43,51.[doi:DOI:10.3969/j.issn.1000-7377.2025.01.007]
　HE Jingwen,LI Dejia,ZHANG Yi,et al.Effect of platelet-rich plasma microspheres on urodynamics and collagen metabolism of anterior vaginal wall in rats with urinary incontinence after radical cervical cancer surgery[J].,2025,54(1):38-43,51.[doi:DOI:10.3969/j.issn.1000-7377.2025.01.007]
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富血小板血浆微球对宫颈癌根治术后尿失禁大鼠尿动力学及阴道前壁胶原代谢影响
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
54
期数:
2025年1期
页码:
38-43,51
栏目:
基础研究
出版日期:
2025-01-05

文章信息/Info

Title:
Effect of platelet-rich plasma microspheres on urodynamics and collagen metabolism of anterior vaginal wall in rats with urinary incontinence after radical cervical cancer surgery
作者:
贺静雯1; 李得加1; 张 毅2; 闫益芳2
(1.锦州医科大学,辽宁 锦州 121001; 2.十堰市人民医院,湖北 十堰 442000)
Author(s):
HE Jingwen; LI Dejia; ZHANG Yi; YAN Yifang
(Jinzhou Medical University,Jinzhou 121001,China)
关键词:
富血小板血浆微球;  宫颈癌根治术后;  尿失禁;  尿动力学;  阴道前壁胶原代谢
Keywords:
Platelet-rich plasma microspheres;  After radical surgery of cervical cancer;  Urinary incontinence;  Urodynamics;  Collagen metabolism in anterior vaginal wall
分类号:
R 713.4
DOI:
DOI:10.3969/j.issn.1000-7377.2025.01.007
文献标志码:
A
摘要:
目的:本研究旨在探讨富血小板血浆微球对宫颈癌根治术后尿失禁大鼠尿动力学及阴道前壁胶原代谢影响。方法:将60只6～8周未生产的雌性大鼠按随机数字表法分为正常组、模型组、富血小板血浆组、空白明胶微球组及富血小板血浆微球组各12只,制备宫颈癌根治术后尿失禁大鼠模型成功后,正常组、模型组正常饲养,其余三组依次采用微量注射器注入富血小板血浆、空白明胶微球及富血小板血浆微球。观察各组大鼠一般情况、尿动力指标、膀胱内压、尿道括约肌组织形态、阴道前壁转化生长因子-β(TGF-β)、金属蛋白酶组织抑制剂-1(TIMP-1)、基质金属蛋白酶-1(MMP-1)、基质金属蛋白酶-9(MMP-9)、Ⅰ型胶原蛋白(Col-Ⅰ)信使核糖核酸(mRNA)表达水平、TGF-β、TIMP-1、MMP-1、MMP-9、Col-Ⅰ 蛋白表达。结果:与模型组比较,正常组、富血小板血浆组、空白明胶微球组及富血小板血浆微球组尿动力指标、膀胱内压、阴道前壁TGF-β mRNA/蛋白、TIMP-1 mRNA/蛋白、MMP-1 mRNA/蛋白、MMP-9 mRNA/蛋白、Col-Ⅰ mRNA/蛋白均改善(均P<0.05); 与富血小板血浆组、空白明胶微球组比较,正常组及富血小板血浆微球组尿动力指标、膀胱内压、阴道前壁TGF-β mRNA/蛋白、TIMP-1 mRNA/蛋白、MMP-1 mRNA/蛋白、MMP-9 mRNA/蛋白、Col-Ⅰ mRNA/蛋白均改善(均P<0.05); 与富血小板血浆微球组比较,正常组尿动力指标、膀胱内压、阴道前壁TGF-β mRNA/蛋白、TIMP-1 mRNA/蛋白、MMP-1 mRNA/蛋白、MMP-9 mRNA/蛋白、Col-Ⅰ mRNA/蛋白均改善(均P<0.05); 富血小板血浆组与空白明胶微球组比较,差异有统计学意义(均P>0.05)。结论:富血小板血浆微球能够恢复宫颈癌根治术后尿失禁大鼠尿动力学,同时能够调节胶原代谢。
Abstract:
Objective:To investigate the effects of platelet-rich plasma microspheres on urodynamics and collagen metabolism of anterior vaginal wall in rats with urinary incontinence after radical cervical cancer surgery.Methods:A total of 60 female rats that had not given birth for 6 to 8 weeks were divided into normal group,model group,platelet-rich plasma group,blank gelatin microsphere group and platelet-rich plasma microsphere group with 12 rats in each group according to random number table method.After successful preparation of urinary incontinence rat model after radical cervical cancer surgery,normal group and model group were fed normally,and the other three groups were injected with platelet-rich plasma,blank gelatin microspheres and platelet-rich plasma microspheres with microsyringes.The general condition,urinary dynamic indexes,internal bladder pressure,urethral sphincter tissue morphology,anterior vaginal wall transforming growth factor-β(TGF-β),tissue inhibitor of metalloproteinase-1(TIMP-1),matrix metalloproteinase-1(MMP-1),matrix metalloproteinase-9(MMP-9),type Ⅰ collagen(Col-Ⅰ mRNA expression level,TGF-β,TIMP-1,MMP-1,MMP-9,Col-Ⅰ protein expression.were observed in each group.Results:Compared with the model group,urinary motility indexes,bladder internal pressure,TGF-β mRNA and protein,TIMP-1 mRNA and protein,MMP-1 mRNA and protein,MMP-9 mRNA and protein,Col-Ⅰ mRNA and protein in normal group,platelet-rich plasma group,blank gelatin microsphere group and platelet-rich plasma microsphere group mRNA and protein were improved(all P<0.05).Compared with platelet-rich plasma group and blank gelatin microsphere group,urinary motility index,bladder internal pressure,TGF-β mRNA and protein,TIMP-1 mRNA and protein,MMP-1 mRNA and protein,MMP-9 mRNA and protein,Col-Ⅰ mRNA and protein were improved in normal group and platelet-rich plasma micropellet group(all P<0.05).Compared with platelet-rich plasma micropellet group,urinary motility index,bladder pressure,TGF-β mRNA and protein,TIMP-1 mRNA and protein,MMP-1 mRNA and protein,MMP-9 mRNA and protein,Col-Ⅰ mRNA and protein in normal group were improved(all P<0.05).The above indexes in platelet-rich plasma group were not different from blank gelatin microsphere group(all P>0.05).Conclusion:Platelet-rich plasma microspheres can restore urodynamics and regulate collagen metabolism in rats with urinary incontinence after radical cervical cancer surgery.

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备注/Memo

备注/Memo:
基金项目:湖北省教育厅科学研究计划指导项目(B2021448)
更新日期/Last Update: 2025-01-06