[1]李冬雅,周婷婷,葛殿奎.VEGFR-3、MMP-9、nm23-H1蛋白在口腔癌组织中的表达及其临床价值研究[J].陕西医学杂志,2024,(11):1558-1561,1566.[doi:DOI:10.3969/j.issn.1000-7377.2024.11.026]
　LI Dongya,ZHOU Tingting,GE Diankui.Expression and clinical significance of VEGFR-3,MMP-9 and nm23-H1 proteins in oral cancer tissue[J].,2024,(11):1558-1561,1566.[doi:DOI:10.3969/j.issn.1000-7377.2024.11.026]
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VEGFR-3、MMP-9、nm23-H1蛋白在口腔癌组织中的表达及其临床价值研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2024年11期
页码:
1558-1561,1566
栏目:
临床病理
出版日期:
2024-11-05

文章信息/Info

Title:
Expression and clinical significance of VEGFR-3,MMP-9 and nm23-H1 proteins in oral cancer tissue
作者:
李冬雅; 周婷婷; 葛殿奎
(徐州医科大学附属淮安医院口腔科,江苏 淮安 223001)
Author(s):
LI Dongya; ZHOU Tingting; GE Diankui
(Department of Stomatology,the Affiliated Huai'an Hospital of Xuzhou Medical University,Huai'an 223001,China)
关键词:
口腔癌;  血管内皮生长因子受体3;  基质金属蛋白酶-9;  nm23-H1;  临床病理特征;  预后
Keywords:
Oral cancer;  Vascular endothelial growth factor receptor 3;  Matrix metalloproteinase-9;  nm23-H1;  Clinical pathological characteristics;  Prognosis
分类号:
R 739.85
DOI:
DOI:10.3969/j.issn.1000-7377.2024.11.026
文献标志码:
A
摘要:
目的:探讨血管内皮生长因子受体3(VEGFR-3)、基质金属蛋白酶-9(MMP-9)、nm23-H1蛋白在口腔癌组织中的表达及其临床价值。方法:选取行手术治疗的口腔鳞状细胞癌患者70例,收集癌组织标本。另选取同期体检健康者70例,采集口腔黏膜组织。采用免疫组织化学染色法检测样本组织VEGFR-3、MMP-9、nm23-H1蛋白表达,分析其与患者临床病理特征的相关性。术后随访5年,记录患者生存情况。分析VEGFR-3、MMP-9、nm23-H1蛋白表达与患者术后5年预后的关系。结果:口腔癌组织VEGFR-3、MMP-9蛋白阳性表达率高于口腔黏膜组织,nm23-H1蛋白阳性表达率低于口腔黏膜组织(均P<0.05)。VEGFR-3、MMP-9蛋白阳性表达患者淋巴结转移比例较高(均P<0.05)。nm23-H1蛋白阳性表达患者淋巴结转移、TNM分期Ⅲ期-Ⅳ期及肿瘤浸润深度>5 mm比例较低(均P<0.05)。口腔癌组织VEGFR-3、MMP-9蛋白表达与淋巴结转移呈正相关(均P<0.05)。m23-H1蛋白表达与淋巴结转移、TNM分期、肿瘤浸润深度呈负相关(均P<0.05)。VEGFR-3、MMP-9蛋白阳性表达患者5年生存率低于阴性表达患者(均P<0.05)。m23-H1蛋白阳性表达患者5年生存率高于阴性表达患者(P<0.05)。结论:口腔癌组织VEGFR-3、MMP-9蛋白高表达,nm23-H1蛋白低表达,三者与淋巴结转移以及口腔癌患者预后有关。
Abstract:
Objective:To explore the expression of vascular endothelial growth factor receptor 3(VEGFR-3),matrix metalloproteinase-9(MMP-9)and nm23-H1 protein in oral cancer tissue and their clinical significance.Methods:Seventy patients with oral squamous cell carcinoma who underwent surgical treatment were selected,and cancer tissue specimens were collected.Additionally,oral mucosal tissues were collected from 70 healthy individuals during the same period.Immunohistochemical staining was used to detect the expression of VEGFR-3,MMP-9 and nm23-H1 proteins in the tissue samples,and their correlation with the clinical pathological characteristics of the patients was analyzed.The patients were followed up for 5 years postoperatively to record their survival status and to analyze the relationship between the expression of VEGFR-3,MMP-9 and nm23-H1 proteins and the patients' prognosis after 5 years.Results:The positive expression rates of VEGFR-3 and MMP-9 proteins in oral cancer tissue were higher than those in oral mucosal tissue,while the positive expression rate of nm23-H1 protein was lower than that in oral mucosal tissue(all P<0.05).Patients with positive expression of VEGFR-3 and MMP-9 proteins had a higher proportion of lymph node metastasis(all P<0.05).Patients with positive expression of nm23-H1 protein had a lower proportion of lymph node metastasis,TNM stage Ⅲ-Ⅳ and tumor invasion depth >5 mm(all P<0.05).The expression of VEGFR-3 and MMP-9 proteins in oral cancer tissue was positively correlated with lymph node metastasis(all P<0.05).The expression of nm23-H1 protein was negatively correlated with lymph node metastasis,TNM stage,and tumor invasion depth(all P<0.05).Patients with positive expression of VEGFR-3 and MMP-9 proteins had a lower 5-year survival rate than those with negative expression(all P<0.05).Patients with positive expression of nm23-H1 protein had a higher 5-year survival rate than those with negative expression(P<0.05).Conclusion:High expression of VEGFR-3 and MMP-9 proteins and low expression of nm23-H1 protein in oral cancer tissue are associated with lymph node metastasis and the prognosis of patients with oral cancer.

参考文献/References:

[1] 韦敏,顾春梅,管燕华,等.miR-21对人口腔癌细胞株HSQ-89增殖、侵袭与迁移的影响实验研究[J].陕西医学杂志,2024,53(2):157-162.
[2] 高宾,王春花,李雅琪,等.长链非编码RNA在口腔鳞状细胞癌及其微环境中的作用研究进展[J].陕西医学杂志,2022,51(11):1458-1461.
[3] 周瑜,段贤捷,翁海燕,等.浸润深度在口腔鳞状细胞癌预后中的价值[J].实用肿瘤杂志,2023,38(2):173-179.
[4] 王静璇,刘红悦,庄志征,等.MMP-9抑制剂对人口腔鳞状细胞癌细胞系CAL27生物学行为影响机制的研究[J].口腔颌面外科杂志,2023,33(6):363-369.
[5] OBARA N,KYAKUMOTO S,YAMAGUCHI S,et al.Disruption of CADM1-dependent cell-cell adhesion in human oral squamous cell carcinoma cells results in tumor progression,possibly through an increase of MMP-2 and MMP-9 expression[J].J Oral Biosci,2024,66(1):151-159.
[6] 王沛靓,刘雁林.3种不同标志物对人淋巴管道内皮细胞鉴定效果研究[J].现代医药卫生,2024,40(14):2341-2343.
[7] 赵辉,王雷,冯跃庆.Nm23-H1对乳腺癌细胞上皮-间充质转化、侵袭和转移的影响[J].中华实验外科杂志,2021,38(7):1272-1274.
[8] LU Z,LIU B,KONG D,et al.NSUN6 regulates NM23-H1 expression in an m5C manner to affect epithelial-mesenchymal transition in lung cancer[J].Med Princ Pract,2024,33(1):56-65.
[9] 杨立平,李晓峰,秦伟,等.结直肠癌组织中Survivin、MMP-2、nm23-H1及VEGF的表达及临床意义[J].中国老年学杂志,2017,37(19):4819-4821.
[10] 王利伟,初桂伟,杜灵霞,等.nm23-H1蛋白表达情况与舌癌浸润转移的关系[J].解放军医药杂志,2018,30(10):20-22.
[11] 周婷婷,谭劲,吴丹,等.姜黄素对人口腔黏膜上皮细胞系增殖及Wnt/β-catenin信号通路的影响[J].陕西中医,2022,43(9):1155-1158.
[12] 王润堃,陆汉强,黄秋生.口腔癌调强放疗中靶区优化对患者口腔黏膜反应、唾液腺功能的影响[J].中国医学物理学杂志,2024,41(2):145-150.
[13] 曹俊华,李冠娥,王丽艳,等.口腔癌患者淋巴结转移分布与淋巴结靶区勾画范围的一致性分析[J].中国CT和MRI杂志,2023,21(6):47-49.
[14] 戴银花,戴群,邹文英,等.HIF-1α对口腔癌裸鼠移植瘤生长及其CEACAM1及VEGF-C表达的影响[J].实用口腔医学杂志,2017,33(4):459-463.
[15] 叶鑫,王梦涵,袁婉婧,等.健脾补肾法对人结直肠癌裸鼠VEGF-C、VEGFR-3、E-cadherin、Vimentin表达的影响[J].中医药信息,2023,40(11):39-42,52.
[16] 李乐,王斌全,田俊,等.血管内皮生长因子受体-3抗体对人喉鳞癌裸鼠移植瘤生长的影响[J].中国药物与临床,2017,17(2):168-170.
[17] BRANDT E,KESKIN M,RÄISÄNEN I T,et al.Induction of collagenolytic MMP-8 and -9 tissue destruction cascade in mouth by head and neck cancer radiotherapy:A cohort study[J].Biomedicines,2023,12(1):27.
[18] 李向阳,任磊,梁瑞.血清基质金属蛋白酶-9、基质金属蛋白酶-2水平对胃癌腹膜转移及预后的预测价值[J].陕西医学杂志,2023,52(2):166-170.
[19] 丁丽娜,于烁,傅志华,等.口腔鳞癌中IL-17和MMP-9的表达及意义[J].中国实验诊断学,2018,22(4):612-615.
[20] 高瑞琪,姜智雯,秦高伟,等.MMP-9在口腔癌转移中的研究进展[J].中国实验诊断学,2022,26(9):1411-1413.
[21] 王阳,高建超,李华,等.胃癌患者组织SOCS-1和nm23-H1基因差异表达与临床参数的相关因素研究[J].现代消化及介入诊疗,2023,28(10):1255-1258.
[22] 高辉,张雪,刘文月.斯钙素2、转移抑制基因23-H1在宫颈癌组织中的表达及临床意义[J].癌症进展,2021,19(5):483-486.
[23] LEE S Y,PARK H R,CHO N H,et al.Identifying genes related to radiation resistance in oral squamous cell carcinoma cell lines[J].Int J Oral Maxillofac Surg,2013,42(2):169-176.
[24] 任柯,刘清泉,张亚猛,等.微小RNA-146a靶向Nm23-H1对胃印戒细胞癌侵袭和转移的影响[J].中华实验外科杂志,2021,38(3):442-445.
[25] 王利伟,初桂伟,杜灵霞,等.nm23-H1 mRNA在舌癌中的表达及其与肿瘤侵袭和转移的关系[J].解放军预防医学杂志,2018,36(7):925-928.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81600145)
更新日期/Last Update: 2024-11-04