[1]陈 慧,何启强,谢 胜,等.蛋白激酶C-α/Kelch样环氧氯丙烷相关蛋白1/核转录因子E2相关因子2/时核因子κB信号通路在慢性非细菌性前列腺炎小鼠中的表达及作用机制[J].陕西医学杂志,2024,(10):1320-1325.[doi:DOI:10.3969/j.issn.1000-7377.2024.10.005]
　CHEN Hui,HE Qiqiang,XIE Sheng,et al.Expression and mechanism of PKC-α/Keap1/Nrf2/NF-κB signaling pathway in chronic nonbacterial prostatitis in mice[J].,2024,(10):1320-1325.[doi:DOI:10.3969/j.issn.1000-7377.2024.10.005]
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蛋白激酶C-α/Kelch样环氧氯丙烷相关蛋白1/核转录因子E2相关因子2/时核因子κB信号通路在慢性非细菌性前列腺炎小鼠中的表达及作用机制
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2024年10期
页码:
1320-1325
栏目:
基础研究
出版日期:
2024-10-05

文章信息/Info

Title:
Expression and mechanism of PKC-α/Keap1/Nrf2/NF-κB signaling pathway in chronic nonbacterial prostatitis in mice
作者:
陈 慧1; 何启强1; 谢 胜2; 欧阳柳2
(1.锦州医科大学,辽宁 锦州,121000; 2.十堰市人民医院,湖北 十堰 442000)
Author(s):
CHEN Hui; HE Qiqiang; XIE Sheng; OU Yangliu
(Jinzhou Medical University,Jinzhou 121000,China)
关键词:
PKC-α/Keap1/Nrf2/NF-κB信号通路;  前列腺素E2;  环氧化酶-2;  慢性非细菌性前列腺炎;  前列腺指数;  前列腺组织病理形态;  前列腺病理改变评分
Keywords:
PKC-α/Keap1/Nrf2/NF-κB signaling pathway;  PGE2;  COX-2;  Chronic nonbacterial prostatitis;  Prostatic index;  Pathological morphology of prostate tissue;  Pathological change score of prostate
分类号:
R 697.3
DOI:
DOI:10.3969/j.issn.1000-7377.2024.10.005
文献标志码:
A
摘要:
目的:本研究旨在探讨蛋白激酶C-α(PKC-α)/Kelch样环氧氯丙烷相关蛋白1(Keap1)/核转录因子E2相关因子2(Nrf2)/时核因子κB(NF-κB)信号通路在小鼠慢性非细菌性前列腺炎中的表达及作用机制。方法:60只4～6周龄裸鼠,体重(180±20)g,随机分为空白对照组、模型组及慢性非细菌性前列腺炎组,每组20只。其中空白组正常饲养; 模型组仅造模前禁食不禁水12 h,将小鼠麻醉(0.8%戊巴比妥0.5 ml/100 g)后,将腹部毛发剔除,常规消毒,将阴囊皮肤剪开后缝合。慢性非细菌性前列腺炎组持续将双侧睾丸取出将精索血管结扎,缝合伤口,连续3 d给予30000μm/kg庆大霉素防止感染。制备慢性非细菌性前列腺炎:术后次日0.25 mg/kg苯甲醛雌二醇经小鼠背部皮下注射,1次/d,连续1个月。比较各组小鼠前列腺指数,前列腺组织病理形态及前列腺病理改变评分,分析各组小鼠PKC-α/Keap1/Nrf2/p-NF-κB p65信号通路及血清前列腺素E2(PGE2)、环氧化酶-2(COX-2)表达变化情况。结果: 慢性非细菌性前列腺炎组体重、前列腺质量、前列腺指数低于空白对照组、模型组(均P<0.05); 空白对照组与模型组体重、前列腺质量、前列腺指数比较无统计学差异(均P>0.05)。空白对照组及模型组,前列腺组织腺腔有条理、结构清晰,间质内不存在炎性细胞浸润和扩张; 慢性非细菌性前列腺炎组,不同形态的前列腺组织腺腔不存在腔内分泌物、缩小的胞腔,间质内被大量炎性细胞弥漫性浸润、呈疏松状,存在大量纤维组织增生。慢性非细菌性前列腺炎组前列腺病理改变评分高于空白对照组、模型组(均P<0.05); 空白对照组与模型组前列腺病理改变评分比较差异有统计学意义(P>0.05)。慢性非细菌性前列腺炎组PKC-α、p-NF-κB p65高于空白对照组、模型组,Keap1、Nrf2低于空白对照组、模型组(均P<0.05); 空白对照组与模型组PKC-α、Keap1、Nrf2、p-NF-κB p65比较无统计学差异(均P>0.05)。慢性非细菌性前列腺炎组PGE2、COX-2表达高于空白对照组、模型组(均P<0.05); 空白对照组与模型组PGE2、COX-2表达比较无统计学差异(均P>0.05)。结论: PKC-α/Keap1/Nrf2/NF-κB信号通路在小鼠慢性非细菌性前列腺炎中呈异常表达,这可能是慢性非细菌性前列腺炎发生、发展的作用机制之一。
Abstract:
Objective:To investigate the expression and mechanism of protein kinase C-α(PKC-α)/Kelch-like epiglorohydrin associated protein 1(Keap1)/nuclear transcription factor E2-associated factor 2(Nrf2)/nuclear factor κB(NF-κB)signaling pathway in chronic nonbacterial prostattis in mice.Methods:Sixty nude mice aged 4 to 6 weeks with body weight of(180±20)g were purchased and randomly divided into blank control group,model group and chronic nonbacterial prostatitis group with 20 mice each.The blank group was fed normally.The model group was only deprived of water for 12 hours before modeling.The mice were anesthetized(0.8% pentobarbital 0.5 ml/100 g),the abdominal hair was removed,routine disinfection was performed,and the scrotal skin was cut and sutured.In the chronic nonbacterial prostaglitis group,both testicles were removed continuously,the spermatic cord vessels were ligated,the wound was sutured,and 30000 μm/kg gentamicin was given for 3 consecutive days to prevent infection.Preparation of chronic nonbacterial prostatitis:0.25 mg/kg benzaldehyde estradiol was injected subcutaneously into the back of mice on the second day after surgery,once a day for 1 month.The prostatic index,pathological morphology and pathological change score of prostate were compared in each group.PKC-α/Keap1/Nrf2/p-NF-κB p65 signaling pathway and the expression of prostaglandin E2(PGE2)and cycoperoxidase 2(COX-2)in serum were analyzed.Results:Body mass,prostate mass and prostate index in chronic nonbacterial prostatitis group were lower than those in blank control group and model group(all P<0.05).Body mass,prostate mass and prostate index in blank control group were not different from model group(all P>0.05).In the blank control group and the model group,the gland cavity of prostate tissue was organized,the structure was clear,there was no inflammatory cell infiltration and expansion in the interstitium,and there was no pathological change.In the chronic nonbacterial prostatitis group,there was no intracavitary secretion in the gland cavity of the prostate tissue of different forms,and there was a small cell cavity,and the interstitium was diffusely infiltrated by a large number of inflammatory cells,showing a loose shape and a large number of fibrous tissue hyperplasia.The score of pathological changes of prostate in chronic nonbacterial prostatitis group was higher than that in blank control group and model group(all P<0.05).There was significant difference in the score of pathological changes of prostate between blank control group and model group(P>0.05).PKC-α and P-NF-κB p65 in chronic nonbacterial prostatitis group were higher than those in blank control group and model group,Keap1 and Nrf2 were lower than those in blank control group and model group(all P<0.05).PKC-α,Keap1,Nrf2 and P-NF-κB p65 in blank control group were not different from model group(all P>0.05).The expressions of PGE2 and COX-2 in chronic nonbacterial prostatitis group were higher than those in blank control group and model group(all P<0.05).There were significant differences in the expression of PGE2 and COX-2 between blank control group and model group(all P>0.05).Conclusion:PKC-α/Keap1/Nrf2/NF-κB signaling pathway is abnormally expressed in chronic nonbacterial prostatitis in mice,which may be one of the mechanisms of the occurrence and development of chronic nonbacterial prostatitis.

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备注/Memo

备注/Memo:
基金项目:湖北省教育厅科学研究计划指导性项目(Q20212102)
更新日期/Last Update: 2024-10-08