[1]纪伟超,郭红英,李曼曼,等.人类白细胞抗原-DQA1、细胞色素P4503A5*3基因多态性与特发性膜性肾病遗传易感性关联性研究[J].陕西医学杂志,2024,(7):936-939.[doi:DOI:10.3969/j.issn.1000-7377.2024.07.015]
　JI Weichao,GUO Hongying,LI Manman,et al.Correlation between gene polymorphisms of HLA-DQA1 and CYP3A5*3 and genetic susceptibility of idiopathic membranous nephropathy[J].,2024,(7):936-939.[doi:DOI:10.3969/j.issn.1000-7377.2024.07.015]
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人类白细胞抗原-DQA1、细胞色素P4503A5*3基因多态性与特发性膜性肾病遗传易感性关联性研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2024年7期
页码:
936-939
栏目:
临床研究
出版日期:
2024-07-05

文章信息/Info

Title:
Correlation between gene polymorphisms of HLA-DQA1 and CYP3A5*3 and genetic susceptibility of idiopathic membranous nephropathy
作者:
纪伟超1; 郭红英2; 李曼曼3; 成雪红3; 任美芳4
(1.河北以岭医院肾内科,河北 石家庄 050090; 2.石家庄市第二医院肾内科,河北 石家庄 050000; 3.石家庄市藁城人民医院肾内科,河北 藁城 052160; 4.河北省中医院肾病一科,河北 石家庄 050000)
Author(s):
JI Weichao; GUO Hongying; LI Manman; CHENG Xuehong; REN Meifang
(Department of Nephrology,Hebei Yiling Hospital,Shijiazhuang 050090,China)
关键词:
人类白细胞抗原-DQA1;  细胞色素P4503A5*3;  基因多态性;  特发性膜性肾病;  遗传易感性;  关联性
Keywords:
Human leukocyte antigen-DQA1;  Cytochrome P4503A5*3;  Gene polymorphism;  Idiopathic membranous nephropathy;  Genetic susceptibility;  Relevance
分类号:
R 692
DOI:
DOI:10.3969/j.issn.1000-7377.2024.07.015
文献标志码:
A
摘要:
目的:探讨分析人类白细胞抗原(HLA)-DQA1、细胞色素P450(CYP)3A5*3基因多态性与特发性膜性肾病(IMN)遗传易感性的关联性。方法:将IMN患者62例作为研究组,另将健康者62例作为对照组。采用聚合酶链反应(PCR)技术和基因测序技术检测入组患者的HLA-DQA1(rs2187668)、CYP3A5*3(rs776746)基因型,判断各基因型与IMN患者的关系。结果:研究组中HLA-DQA1 rs2187668基因型AA占比高于对照组(P<0.05),基因型GG、AG占比低于对照组(均P<0.05); 研究组等位基因A占比高于对照组(P<0.05)。研究组与对照组CYP3A5*3 rs776746不同基因型占比及等位基因占比比较无统计学差异(均P>0.05)。HLA-DQA1 rs2187668AA基因型IMN患者24 h尿蛋白量和血肌酐水平显著高于AG和GG型,肾小球滤过率显著低于AG和GG型(均P<0.05)。结论:HLA-DQA1 rs2187668位点的基因多态性与IMN的发生有关,AA基因型和等位基因A可增加IMN易感性。
Abstract:
Objective:To explore the correlation between gene polymorphisms of human leukocyte antigen(HLA)-DQA1 and cytochrome P450(CYP)3A5*3 and genetic susceptibility of idiopathic membranous nephropathy(IMN).Methods:A total of 62 patients with IMN and 62 healthy controls as study group and control group,respectively.The genotypes of HLA-DQA1(rs2187668)and CYP3A5*3(rs776746)were detected by polymerase chain reaction(PCR)and gene sequencing method,and their relationship with IMN was determined.Results:The proportion of HLA-DQA1 rs2187668 genotype AA in study group was higher than that in control group(P<0.05),and the proportion of GG and AG genotype was lower than that in the control group(all P<0.05).The proportion of allele A in study group was higher than that in control group(P<0.05).There was no significant difference in the proportions of different genotypes and alleles in CYP3A5*3(rs776746)between the two groups(all P>0.05).The levels of 24-hour urinary protein and serum creatinine in IMN patients with HLA-DQA1(rs2187668)'s genotype AA were significantly higher than those with genotypes AG and GG,and glomerular filtration rate was significantly lower than that with genotypes AG and GG(all P<0.05).Conclusion:The polymorphisms of HLA-DQA1 gene at rs2187668 locus are related to the occurrence of IMN,genotype AA and allele A will increase IMN susceptibility.

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备注/Memo

备注/Memo:
基金项目:河北省中医药管理局科研计划项目(2020082)
更新日期/Last Update: 2024-07-04