[1]伍勇彬,黄 山,姚巧玲,等.长链非编码RNA RP11-497E19.1对胃癌细胞增殖和侵袭的影响及机制实验研究[J].陕西医学杂志,2024,(6):729-733.[doi:DOI:10.3969/j.issn.1000-7377.2024.06.002]
　WU Yongbin,HUANG Shan,YAO Qiaoling,et al.Effect of long non-coding RNA RP11-497E19.1 on proliferation and invasion of gastric cancer cells and its mechanism[J].,2024,(6):729-733.[doi:DOI:10.3969/j.issn.1000-7377.2024.06.002]
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长链非编码RNA RP11-497E19.1对胃癌细胞增殖和侵袭的影响及机制实验研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2024年6期
页码:
729-733
栏目:
基础研究
出版日期:
2024-06-05

文章信息/Info

Title:
Effect of long non-coding RNA RP11-497E19.1 on proliferation and invasion of gastric cancer cells and its mechanism
作者:
伍勇彬1; 黄 山1; 姚巧玲1; 张晓伟2
(1.资阳市第一人民医院消化科,四川 资阳 641300; 2.复旦大学附属肿瘤医院肿瘤科,上海 200032)
Author(s):
WU Yongbin; HUANG Shan; YAO Qiaoling; ZHANG Xiaowei
(Department of Gastroenterology,the First People's Hospital of Ziyang,Ziyang 641300,China)
关键词:
胃癌;  长链非编码RNA;  RP11-497E19.1;  微小RNA-545-5p;  细胞增殖;  细胞侵袭;  分子通路
Keywords:
Gastric cancer;  Long non-coding RNA;  RP11-497E19.1;  miR-545-5p;  Cell proliferation;  Cell invasion;  Molecular pathways
分类号:
R 73-3
DOI:
DOI:10.3969/j.issn.1000-7377.2024.06.002
文献标志码:
A
摘要:
目的:探讨长链非编码RNA(lncRNA)RP11-497E19.1对胃癌细胞增殖和侵袭的影响及其机制。方法:实时荧光定量PCR(RT-qPCR)检测胃癌HS-746T、BGC823、NCI-N87、SGC7901、AGS细胞和永生化胃上皮细胞GES-1中RP11-497E19.1表达水平,筛选表达最高的细胞进行后续实验。将HS-746T细胞分为si-NC组和si-RP11-497E19.1组,分别转染阴性对照寡核苷酸或RP11-497E19.1小干扰RNA。集落形成实验和Transwell实验分析转染HS-746T细胞的增殖、侵袭能力。双荧光素酶报告基因实验验证RP11-497E19.1与微小RNA(miR)-545-5p的靶向关系。RT-qPCR检测转染HS-746T细胞miR-545-5p的表达。Western blot检测转染HS-746T细胞间质表皮转化因子(c-Met)/胆绿素还原酶(BVR)/活化复制因子2(ATF-2)分子通路相关蛋白的表达。结果:与GES-1细胞比较,HS-746T、BGC823、NCI-N87、SGC7901、AGS细胞中RP11-497E19.1表达水平均上升,且HS-746T细胞RP11-497E19.1表达水平最高(均P<0.05)。与si-NC组比较,si-RP11-497E19.1组HS-746T细胞活力和细胞侵袭数降低(均P<0.05)。双荧光素酶报告基因实验证实RP11-497E19.1靶向结合miR-545-5p,可负向调控miR-545-5p表达(P<0.05)。与si-NC组比较,si-RP11-497E19.1组HS-746T细胞c-Met/BVR/ATF-2分子通路蛋白c-Met、BVR、p-ATF-2、锌指蛋白1(Snail1)、锌指蛋白2(Snail2)表达降低(均P<0.05)。结论:胃癌细胞中RP11-497E19.1呈高表达,沉默RP11-497E19.1通过靶向miR-545-5p/c-Met/BVR/ATF-2分子通路抑制胃癌HS-746T细胞的增殖及侵袭。
Abstract:
Objective:To investigate the effect of lncRNA RP11-497E19.1 on the proliferation and invasion of gastric cancer cells and its mechanism.Methods:RT-qPCR was used to detect the expression of RP11-497E19.1 in gastric cancer cell lines HS-746T,BGC823,NCI-N87,SGC7901,AGS and immortalized gastric epithelial cell line GES-1,and the cells with the highest expression were selected for subsequent experiments.HS-746T cells were divided into si-NC group and si-RP11-497E19.1 group,which were transfected with negative control oligonucleotide or RP11-497E19.1 small interfering RNA,respectively.The proliferation and invasion ability were analyzed by colony formation assay and Transwell assay.The targeting relationship between RP11-497E19.1 and miR-545-5p was verified by dual luciferase reporter gene assay.The expression of miR-545-5p was detected by RT-qPCR.The expression of c-Met/BVR/ATF-2 pathway related proteins were detected by Western blot.Results: Compared with GES-1 cells,the expression levels of RP11-497E19.1 in HS-746T,BGC823,NCI-N87,SGC7901 and AGS cells were increased,and the expression level of RP11-497E19.1 in HS-746T cells was the highest(all P<0.05).Compared with the si-NC group,the proliferation and invasive number of HS-746T cells in the si-RP11-497E19.1 group were decreased(all P<0.05).Dual luciferase reporter gene assay confirmed that RP11-497E19.1 targeted miR-545-5p and negatively regulated the expression of miR-545-5p(P<0.05).Compared with the si-NC group,the expression of c-Met,BVR,p-ATF-2,Snail1 and Snail2 proteins in the c-Met/BVR/ATF-2 molecular pathway in HS-746T cells in the si-RP11-497E19.1 group decreased(all P<0.05).Conclusion:RP11-497E19.1 is highly expressed in gastric cancer cells.Silencing RP11-497E19.1 inhibits the proliferation and invasion of gastric cancer HS-746T cells by targeting the miR-545-5p/c-Met/BVR/ATF-2 molecular pathway.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81871948)
更新日期/Last Update: 2024-06-05