[1]姚 云,廖 冬,周双雄,等.长链非编码RNA CYP1B1-AS1对急性髓系白血病细胞增殖和迁移的影响及机制实验研究[J].陕西医学杂志,2024,(6):723-728.[doi:DOI:10.3969/j.issn.1000-7377.2024.06.001]
　YAO Yun,LIAO Dong,ZHOU Shuangxiong,et al.Effect and mechanism of long non-coding RNA CYP1B1-AS1on proliferation and migration of acute myeloid leukemia cells[J].,2024,(6):723-728.[doi:DOI:10.3969/j.issn.1000-7377.2024.06.001]
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长链非编码RNA CYP1B1-AS1对急性髓系白血病细胞增殖和迁移的影响及机制实验研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2024年6期
页码:
723-728
栏目:
基础研究
出版日期:
2024-06-05

文章信息/Info

Title:
Effect and mechanism of long non-coding RNA CYP1B1-AS1on proliferation and migration of acute myeloid leukemia cells
作者:
姚 云1; 廖 冬1; 周双雄1; 刘文俐1; 王 荣2
(1.荆楚理工学院附属中心医院 荆门市人民医院血液内科,湖北 荆门 448000; 2.郑州大学附属第一医院肿瘤科,河南 郑州 450052)
Author(s):
YAO Yun; LIAO Dong; ZHOU Shuangxiong; LIU Wenli; WANG Rong
(Department of Hematology,Central Hospital Affiliated to Jingchu University of Technology,Jingmen 448000,China)
关键词:
急性髓系白血病;  长链非编码RNA CYP1B1-AS1;  微小RNA-1306-5p;  增殖;  迁移;  靶向关系
Keywords:
Acute myeloid leukemia;  Long non-coding RNA CYP1B1-AS1;  miR-1306-5p;  Proliferation;  Migration;  Targeting relationship
分类号:
R 73-3
DOI:
DOI:10.3969/j.issn.1000-7377.2024.06.001
文献标志码:
A
摘要:
目的:探讨长链非编码RNA(lncRNA)CYP1B1-AS1对急性髓系白血病细胞增殖和迁移的影响及机制。方法:实时荧光定量PCR(RT-qPCR)检测CYP1B1-AS1在急性髓系白血病细胞系THP-1、NB4、HL-60、KG-1、SKM-1和骨髓基质细胞HS-5中的表达量。通过5-氮杂-2'-脱氧胞苷(5-Aza-CdR)处理急性髓系白血病细胞系, RT-qPCR检测5-Aza-CdR对CYP1B1-AS1表达的影响。根据转染物不同分别将HL-60细胞分为NC组和CYP1B1-AS1组。克隆形成实验和划痕实验依次检测HL-60细胞的增殖和迁移能力。双荧光素酶实验验证CYP1B1-AS1与微小RNA(miR)-1306-5p的靶向关系。RT-qPCR检测CYP1B1-AS1对HL-60细胞miR-1306-5p表达的影响。免疫印迹法检测CYP1B1-AS1对HL-60细胞周期蛋白依赖性激酶2(CDK2)、细胞周期素E(Cyclin E)和转录因子(Twist)、纤维连接蛋白(Fibronectin)表达的影响。结果:与HS-5细胞比较,CYP1B1-AS1在急性髓系白血病细胞系中表达量降低,且HL-60细胞系中CYP1B1-AS1表达量最低(均P<0.05)。5-Aza-CdR能够明显促进急性髓系白血病细胞系中CYP1B1-AS1的表达(均P<0.05)。与NC组比较,CYP1B1-AS1组HL-60细胞增殖能力和迁移率降低(均P<0.05)。过表达miR-1306-5p能够抑制野生型CYP1B1-AS1的荧光素酶活性(P<0.05)。与NC组比较,CYP1B1-AS1组HL-60细胞中miR-1306-5p表达减少,细胞增殖和迁移相关蛋白CDK2、Cyclin E、Twist、Fibronectin表达降低(均P<0.05)。结论:过表达CYP1B1-AS1可能通过靶向下调miR-1306-5p表达抑制急性髓系白血病细胞的增殖和迁移。
Abstract:
Objective:To explore the effect and mechanism of long non-coding RNA(lncRNA)CYP1B1-AS1 on proliferation and migration of acute myeloid leukemia cells.Methods:RT-qPCR was used to detect the expression of CYP1B1-AS1 in acute myeloid leukemia cell lines THP-1,NB4,HL-60,KG-1,SKM-1 and bone marrow stromal cells HS-5.Acute myeloid leukemia cell lines were treated with 5-Aza-CdR,and the effect of 5-Aza-CdR on CYP1B1-AS1 was detected by RT-qPCR.HL-60 cells were divided into NC group and CYP1B1-AS1 group according to different transfectants.Clone formation experiment and scratch experiment were used to detect the proliferation and migration ability of HL-60 cells.Dual-luciferase experiment was used to verify the targeting relationship between CYP1B1-AS1 and miR-1306-5p.RT-qPCR was used to detect the effect of CYP1B1-AS1 on the expression of miR-1306-5p in HL-60 cells.Western blot was performed to detect the effect of CYP1B1-AS1 on the expressions of CDK2,Cyclin E,Twist and Fibronectin in HL-60 cells.Results:Compared with HS-5 cells,the expression of CYP1B1-AS1 was decreased in acute myeloid leukemia cell lines,and the expression of CYP1B1-AS1 was the lowest in HL-60 cell line(all P<0.05).5-Aza-CdR could significantly promote the expression of CYP1B1-AS1 in acute myeloid leukemia cell lines(all P<0.05).Compared with the NC group,the proliferation ability and migration rate of HL-60 cells in the CYP1B1-AS1 group were reduced(all P<0.05).Overexpression of miR-1306-5p could inhibit the luciferase activity of wild-type CYP1B1-AS1(P<0.05).Compared with the NC group,the expression of miR-1306-5p in HL-60 cells in the CYP1B1-AS1 group was decreased,and the expression of cell proliferation and migration related proteins CDK2,Cyclin E,Twist and Fibronectin were decreased(all P<0.05).Conclusion:Overexpression of CYP1B1-AS1 may inhibit the proliferation and migration of acute myeloid leukemia cells by targeted down-regulation of miR-1306-5p expression.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(82002210)
更新日期/Last Update: 2024-06-05