[1]要兆旭,马海滨,刘 琳,等.甘草甜素对喉癌Hep-2细胞侵袭和迁移的影响及机制研究[J].陕西医学杂志,2024,(5):604-609.[doi:DOI:10.3969/j.issn.1000-7377.2024.05.006]
　YAO Zhaoxu,MA Haibin,LIU Lin,et al.Effect of glycyrrhizin on invasion and migration of laryngeal carcinoma Hep-2 cells and its mechanism[J].,2024,(5):604-609.[doi:DOI:10.3969/j.issn.1000-7377.2024.05.006]
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甘草甜素对喉癌Hep-2细胞侵袭和迁移的影响及机制研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2024年5期
页码:
604-609
栏目:
基础研究
出版日期:
2024-05-05

文章信息/Info

Title:
Effect of glycyrrhizin on invasion and migration of laryngeal carcinoma Hep-2 cells and its mechanism
作者:
要兆旭; 马海滨; 刘 琳; 赵 倩; 巩 慧; 孙凯丽; 秦隆朝
(邯郸市中心医院耳鼻喉科,河北 邯郸 056002)
Author(s):
YAO Zhaoxu; MA Haibin; LIU Lin; ZHAO Qian; GONG Hui; SUN Kaili; QIN Longchao
(Department of Otolaryngology,Handan Central Hospital,Handan 056002,China)
关键词:
喉癌;  Hep-2细胞;  甘草甜素;  微小RNA-205-5p;  沉默信息调节因子2相关酶3;  侵袭;  迁移
Keywords:
Laryngeal cancer;  Hep-2 cells;  Glycyrrhizin;  microRNA-205-5p;  Silent mating type information regulation 2 homolog-3;  Invasion;  Migration
分类号:
R 36
DOI:
DOI:10.3969/j.issn.1000-7377.2024.05.006
文献标志码:
A
摘要:
目的:探究甘草甜素(Gly)对喉癌Hep-2细胞侵袭和迁移的影响及作用机制。方法:体外培养正常人喉黏膜上皮细胞和喉癌Hep-2细胞,实时荧光定量PCR(RT-qPCR)法和蛋白印迹实验检测微小RNA-205-5p(miR-205-5p)、沉默信息调节因子2相关酶3(SIRT3)mRNA和蛋白表达水平。将喉癌Hep-2细胞分为对照组、Gly低浓度组、Gly中浓度组、Gly高浓度组、Gly高浓度+空载质粒组、Gly高浓度+miR-205-5p抑制剂组。各组给予相应浓度的Gly干预或转染对应质粒培养48 h。Transwell实验和划痕实验分别检测各组Hep-2细胞侵袭和迁移能力。RT-qPCR法和蛋白印迹实验检测各组Hep-2细胞miR-205-5p、SIRT3 mRNA和蛋白表达水平。双荧光素酶报告基因实验分析miR-205-5p与SIRT3的靶向关系。结果:与人喉黏膜上皮细胞比较,喉癌Hep-2细胞miR-205-5p水平降低,SIRT3 mRNA和蛋白水平升高(均P<0.05)。与对照组比较,Gly低、中、高浓度组穿膜细胞数和划痕愈合率、SIRT3 mRNA和蛋白水平依次降低,miR-205-5p水平依次升高(均P<0.05)。与Gly高浓度组和Gly高浓度+空载质粒组比较,Gly高浓度+miR-205-5p抑制剂组穿膜细胞数和划痕愈合率、SIRT3 mRNA和蛋白水平升高,miR-205-5p水平降低(均P<0.05)。miR-205-5p可靶向调控SIRT3表达。结论:Gly能够抑制Hep-2细胞侵袭和迁移,其机制可能与上调miR-205-5p表达,进而靶向下调SIRT3表达有关。
Abstract:
Objective:To explore the effect of glycyrrhizin(Gly)on invasion and migration of laryngeal carcinoma Hep-2 cells and its mechanism.Methods: Normal human laryngeal mucosal epithelial cells and laryngeal cancer Hep-2 cells were cultured in vitro,and the microRNA-205-5p(miR-205-5p),silent mating type information regulation 2 homolog-3(SIRT3)mRNA and protein levels were detected by real-time fluorescent quantitative PCR(RT-qPCR)and western blot assay.Laryngeal cancer Hep-2 cells were divided into control group,Gly low concentration group,Gly medium concentration group,Gly high concentration group,Gly high concentration+empty plasmid group,Gly high concentration+miR-205-5p inhibitor group.Each group was given Gly intervention with corresponding concentration or transfected with corresponding plasmid for 48 h.The invasion and migration ability of Hep-2 cells in each group were detected by Transwell assay and scratch assay respectively.The miR-205-5p,SIRT3 mRNA and protein levels of Hep-2 cells in each group were detected by RT-qPCR and Western blot.The targeting relationship between miR-205-5p and SIRT3 was analyzed by double luciferase reporter gene assay.Results: Compared with human laryngeal mucosal epithelial cells,the levels of miR-205-5p in laryngeal cancer Hep-2 cells were decreased,and the levels of SIRT3 mRNA and protein were increased(all P<0.05).Compared with control group,the number of transmembrane cells,scratch healing rate,SIRT3 mRNA and protein levels in Gly low,medium and high concentration groups were decreased successively,and the levels of miR-205-5p were increased successively(all P<0.05).Compared with Gly high concentration group and Gly high concentration+empty plasmid group,the number of transmembrane cells,scratch healing rate,SIRT3 mRNA and protein levels in Gly high concentration+miR-205-5p inhibitor group were increased,and the level of miR-205-5p was decreased(all P<0.05).miR-205-5p could target the regulation of SIRT3 expression.Conclusion:Gly can inhibit the invasion and migration of Hep-2 cells,and the mechanism may be related to the up-regulation of miR-205-5p expression and the targeted down-regulation of SIRT3 expression.

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相似文献/References:

[1]郭武辉,袁 倩.早期喉癌患者血清膜联蛋白A2、环氧合酶-2及色素框同源物7检测及意义[J].陕西医学杂志,2021,50(11):1446.[doi:DOI:10.3969/j.issn.1000-7377.2021.11.031]

备注/Memo

备注/Memo:
基金项目:河北省医学科学研究课题(20230468)
更新日期/Last Update: 2024-05-06