[1]许卫星,张 薇,陈姣敏,等.芍药苷调节丝氨酸/苏氨酸激酶/鼠双微基因2/p53信号通路对弥漫大B细胞淋巴瘤细胞增殖、凋亡和细胞周期的影响实验研究[J].陕西医学杂志,2024,(3):313-317.[doi:DOI:10.3969/j.issn.1000-7377.2024.03.005]
　XU Weixing,ZHANG Wei,CHEN Jiaomin,et al.Effects of paeoniflorin on proliferation,apoptosis and cell cycle of diffuse large B-cell lymphoma cells by regulating AKT/MDM2/p53 signaling pathway[J].,2024,(3):313-317.[doi:DOI:10.3969/j.issn.1000-7377.2024.03.005]
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芍药苷调节丝氨酸/苏氨酸激酶/鼠双微基因2/p53信号通路对弥漫大B细胞淋巴瘤细胞增殖、凋亡和细胞周期的影响实验研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2024年3期
页码:
313-317
栏目:
基础研究
出版日期:
2024-03-05

文章信息/Info

Title:
Effects of paeoniflorin on proliferation,apoptosis and cell cycle of diffuse large B-cell lymphoma cells by regulating AKT/MDM2/p53 signaling pathway
作者:
许卫星; 张 薇; 陈姣敏; 尹凤雷; 王 娟
(沧州市中心医院血液内一科,河北 沧州 061001)
Author(s):
XU Weixing; ZHANG Wei; CHEN Jiaomin; YIN Fenglei; WANG Juan
(First Department of Hematology,Cangzhou Central Hospital,Cangzhou 061001,China)
关键词:
弥漫大B细胞淋巴瘤;  芍药苷;  丝氨酸/苏氨酸激酶/鼠双微基因2/p53信号通路;  细胞增殖;  细胞凋亡;  细胞周期
Keywords:
Diffuse large B-cell lymphoma;  Paeoniflorin;  AKT/MDM2/p53 signaling pathway;  Cell proliferation;  Apoptosis;  Cell cycle
分类号:
R 285
DOI:
DOI:10.3969/j.issn.1000-7377.2024.03.005
文献标志码:
A
摘要:
目的:探讨芍药苷(PAE)调节丝氨酸/苏氨酸激酶(AKT)/鼠双微基因2(MDM2)/p53信号通路对弥漫大B细胞淋巴瘤(DLBCL)细胞增殖、凋亡和细胞周期的影响。方法:以OCI-LY3细胞为研究对象,分别设置PAE低浓度组(15 μmol/L PAE)、PAE中浓度组(30 μmol/L PAE)、PAE高浓度组(60 μmol/L PAE)、PAE高浓度+ SC79(AKT激活剂)组(60 μmol/L PAE+8 μg/ml SC79),同时以未经处理的细胞为对照组。48 h后,分析细胞增殖、克隆形成能力及细胞周期和凋亡变化,检测AKT/MDM2/p53信号通路相关蛋白的表达水平。结果:与对照组比较,PAE低浓度组、PAE中浓度组、PAE高浓度组细胞凋亡率、p53表达、G0/G1期增加,细胞克隆形成数和细胞增殖率,p-AKT/AKT和p-MDM2/MDM2表达水平,以及G2/M、S期降低(均P<0.05)。与PAE高浓度组比较,PAE高浓度+SC79组细胞凋亡率、p53表达、G0/G1期降低,细胞克隆形成数和细胞增殖率,p-AKT/AKT和p-MDM2/MDM2表达水平,以及G2/M、S期增加(均P<0.05)。结论:PAE通过抑制AKT/MDM2上调p53表达,抑制DLBCL细胞增殖、细胞周期进展,诱导其凋亡。
Abstract:
Objective:To investigate the effects of paeoniflorin on proliferation,apoptosis and cell cycle of diffuse large B-cell lymphoma cells by regulating serine/threonine kinase(AKT)/murine double minute 2(MDM2)/p53 signaling pathway.Methods:OCI-LY3 cells were regarded as the research object,and PAE low concentration group(15 μmol/L PAE),PAE medium concentration group(30 μmol/L PAE),PAE high concentration group(60 μmol/L PAE),and PAE high concentration+ SC79(AKT activator)group(60 μmol/L PAE+8 μg/ml SC79)were set,respectively.At the same time,untreated cells were regarded as the control group.After 48 hours,cell proliferation,colony formation ability,cell cycle and apoptosis were analyzed,and the expression levels of AKT/MDM2/p53 signaling pathway related proteins were detected.Results:Compared with the control group,the apoptosis rate,p53 expression,G0/G1 phase of PAE low concentration group,PAE medium concentration group and PAE high concentration group were obviously increased,the number of cell clones formed,proliferation rate,the expressions of p-AKT/AKT and p-MDM2/MDM2,the G2/M and S phases were obviously reduced(all P<0.05).Compared with the PAE high concentration group,the apoptosis rate,p53 expression,G0/G1 phase of the PAE high concentration+SC79 group were obviously decreased,the number of cell clones formed,proliferation rate,the expressions of p-AKT/AKT and p-MDM2/MDM2,the G2/M and S phases were obviously increased(all P<0.05).Conclusion:PAE can inhibit DLBCL cell proliferation,cell cycle progression,and induce apoptosis by inhibiting AKT/MDM2 expression and up-regulating p53 expression.

参考文献/References:

[1] SUSUANIBAR-ADANIYA S,BARTA S K.2021 update on diffuse large B cell lymphoma:A review of current data and potential applications on risk stratification and management[J].Am J Hematol,2021,96(5):617-629.
[2] 马淑萍,蒙玉娜,党春艳,等.锰超氧化物歧化酶和过氧化物酶6在弥漫性大B细胞淋巴瘤中的表达及临床意义[J].陕西医学杂志,2021,50(9):1154-1157.
[3] LIANG X J,SONG X Y,WU J L,et al.Advances in multi-omics study of prognostic biomarkers of diffuse large B-cell lymphoma[J].Int J Biol Sci,2022,18(4):1313-1327.
[4] PASQULUCCI L,DALLA-FAVERA R.Genetics of diffuse large B-cell lymphoma[J].Blood,2018,131(21):2307-2319.
[5] NIU K,LIU,ZHOU Z,et al.Antitumor effects of paeoniflorin on hippo signaling pathway in gastric cancer cells[J].J Oncol,2021,2021:4724938.
[6] PARK Y S,NAM G H,JO K J,et al.Extract from zanthoxylum piperitum induces apoptosis of AGS gastric cancer cells through AKT/MDM2/p53 signaling pathway[J].Chin J Integr Med,2021,27(10):752-759.
[7] SUN C,LI M,FENG Y,et al.MDM2-P53 signaling pathway-mediated upregulation of CDC20 promotes progression of human diffuse large B-cell lymphoma[J].Onco Targets Ther,2020,13:10475-10487.
[8] ZHANG P,WU N,SONG Z J,et al.Paeoniflorin enhances the sensitivity of ER-positive breast cancer cells to tamoxifen through promoting sirtuin 4[J].Evid Based Complement Alternat Med,2022,2022:6730559.
[9] ZHAO Y,CAI J,SHI K,et al.Germacrone induces lung cancer cell apoptosis and cell cycle arrest via the AKT/MDM2/p53 signaling pathway[J].Mol Med Rep,2021,23(6):452.
[10] LIU H,WEI J,SANG N,et al.The novel LSD1 inhibitor ZY0511 suppresses diffuse large B-cell lymphoma proliferation by inducing apoptosis and autophagy[J].Med Oncol,2021,38(10):124.
[11] ALDERUCCIO J P,SHARMAN J P.ABCs of ADCs in management of relapsed/refractory diffuse large B-cell lymphoma[J].Blood Rev,2022,56:100967.
[12] 王志芳,刘岩,肖丽,等.ICE方案联合利妥昔单抗对难治性弥漫大B细胞淋巴瘤FOXP1与Livin表达影响研究[J].陕西医学杂志,2018,47(1):50-52,68.
[13] 姜菊玲,刘瑞,郑红刚,等.基于中医药现代化抗肿瘤中药新药研发策略探讨和思考[J].中华中医药杂志,2021,36(1):50-55.
[14] NIE X H,QIU S,XING Y,et al.Paeoniflorin regulates NEDD4L/STAT3 pathway to induce ferroptosis in human glioma cells[J].J Oncol,2022,2022:6093216.
[15] SI X L,WANG Y,SONG B N,et al.Potential chemoprevention of paeoniflorin in colitis-associated colorectal cancer by network pharmacology,molecular docking,and in vivo experiment[J].Chem Biodivers,2022,19(8):e202200295.
[16] QIN X,CHEN X,LIU J,et al.Paeoniflorin induces apoptosis and cycle arrest in B-cell acute lymphoblastic leukemia cells by inhibiting SENP1/c-Myc signaling pathway[J].Zhongguo Zhong Yao Za Zhi,2022,47(12):3312-3319.
[17] AHMED S A,MENDONCA P,ELHAG R,et al.Anticancer effects of fucoxanthin through cell cycle arrest,apoptosis induction,angiogenesis inhibition,and autophagy modulation[J].Int J mol Sci,2022,23(24):16091.
[18] SMETS L A.Programmed cell death(apoptosis)and response to anti-cancer drugs[J].Anticancer Drugs,1994,5(1):3-9.
[19] 李娜,邓茜,蒋建军.AKT信号通路在肿瘤中调控机制的研究进展[J].医学综述,2022,28(9):1718-1723.
[20] 马善义,孙兆楼.NVP-BEZ235对人结肠癌细胞SW480体外增殖的抑制作用及其机制探讨[J].陕西医学杂志,2017,46(10):1355-1357.
[21] SONG M,BODE A M,DONG Z,et al.AKT as a therapeutic target for cancer[J].Cancer Res,2019,79(6):1019-1031.
[22] WEI C,DU J,SHEN Y,et al.Anticancer effect of involucrasin A on colorectal cancer cells by modulating the AKT/MDM2/p53 pathway[J].Oncol Lett,2023,25(6):218.
[23] GAO J,XIA R,CHEN J,et al.Inhibition of esophageal-carcinoma cell proliferation by genistein via suppression of JAK1/2-STAT3 and AKT/MDM2/p53 signaling pathways[J].Aging(Albany NY),2020,12(7):6240-6259.
[24] LIU L,YAN J,CAO Y,et al.Proliferation,migration and invasion of triple negative breast cancer cells are suppressed by berbamine via the PI3K/AKT/MDM2/p53 and PI3K/AKT/mTOR signaling pathways[J].Oncol Lett,2021,21(1):70.
[25] HUANG H,PARJ S,ZHANG H,et al.Targeting AKT with costunolide suppresses the growth of colorectal cancer cells and induces apoptosis in vitro and in vivo[J].J Exp Clin Cancer Res,2021,40(1):114.

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备注/Memo

备注/Memo:
基金项目:河北省中医药管理局科研项目(2023454)
更新日期/Last Update: 2024-03-05