[1]戴 琦,崔 宁,刘文卓.黄芩苷调节JAK2/STAT3信号通路对口腔鳞癌细胞增殖、凋亡和侵袭的影响[J].陕西医学杂志,2024,(2):179-183.[doi:DOI:10.3969/j.issn.1000-7377.2024.02.007]
　DAI Qi,CUI Ning,LIU Wenzhuo.Impacts of baicalin on proliferation,apoptosis and invasion of oral squamous cell carcinoma cells by regulating JAK2/STAT3 signaling pathway[J].,2024,(2):179-183.[doi:DOI:10.3969/j.issn.1000-7377.2024.02.007]
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黄芩苷调节JAK2/STAT3信号通路对口腔鳞癌细胞增殖、凋亡和侵袭的影响
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2024年2期
页码:
179-183
栏目:
基础研究
出版日期:
2024-02-05

文章信息/Info

Title:
Impacts of baicalin on proliferation,apoptosis and invasion of oral squamous cell carcinoma cells by regulating JAK2/STAT3 signaling pathway
作者:
戴 琦; 崔 宁; 刘文卓
(济南市中西医结合医院,山东 济南271100)
Author(s):
DAI Qi; CUI Ning; LIU Wenzhuo
(Jinan Hospital of Integrated Traditional Chinese and Western Medicine,Jinan 271100,China)
关键词:
黄芩苷;  JAK2/STAT3信号通路;  口腔鳞癌;  细胞增殖;  细胞凋亡;  细胞侵袭
Keywords:
Baicalin;  JAK2/STAT3 signaling pathway;  Oral squamous cell carcinoma;  Cell proliferation;  Cell apoptosis;  Cell invasion
分类号:
R 739.8
DOI:
DOI:10.3969/j.issn.1000-7377.2024.02.007
文献标志码:
A
摘要:
目的:研究黄芩苷通过调节Janus激酶2(JAK2)/信号转导和转录激活子3(STAT3)信号通路对口腔鳞癌(OSCC)细胞增殖、凋亡、侵袭的影响。方法:体外培养人OSCC细胞CAL27,分为对照组、低浓度黄芩苷组(100 mg/L)、中浓度黄芩苷组(150 mg/L)、高浓度黄芩苷组(200 mg/L)、高浓度黄芩苷(200 mg/L)+Broussonin E组(20 μmol/L JAK2/STAT3激活剂)。然后用集落形成实验检测各组细胞增殖能力,流式细胞仪检测各组细胞凋亡,Transwell法检测各组细胞侵袭能力,ELISA法检测细胞白细胞介素(IL)-18、IL-1β表达水平,Western blot检测各组细胞中磷酸化JAK2(p-JAK2)/JAK2、磷酸化STAT3(p-STAT3)/STAT3、增殖细胞核抗原(PCNA)、Bcl-2相关X蛋白(BAX)、基质金属蛋白酶-9(MMP-9)蛋白表达。结果:与对照组比较,低浓度黄芩苷组、中浓度黄芩苷组、高浓度黄芩苷组CAL27细胞的集落形成率、细胞侵袭数目、细胞中IL-18、IL-1β表达水平、p-JAK2/JAK2、p-STAT3/STAT3、PCNA、MMP-9蛋白表达降低,细胞凋亡率、BAX蛋白表达增加(均P<0.05); 中浓度黄芩苷组、高浓度黄芩苷组与低浓度黄芩苷组相比,以及高浓度黄芩苷组与中浓度黄芩苷组比较与上述结果一致(均P<0.05); 高浓度黄芩苷+Broussonin E组与高浓度黄芩苷组比较,与上述结果趋势相反(P<0.05)。结论:黄芩苷可能通过抑制JAK2/STAT3信号通路对口腔鳞癌细胞的增殖、凋亡和侵袭产生影响。
Abstract:
Objective:To study the impacts of baicalin on proliferation,apoptosis and invasion of oral squamous cell carcinoma(OSCC)cells by regulating Janus kinase 2(JAK2)/signal transducer and activator of transcription 3(STAT3)signaling pathway.Methods:Human OSCC cells CAL27 were cultured in vitro and grouped into control group,low-concentration baicalin group(100 mg/L),medium-concentration baicalin group(150 mg/L),high-concentration baicalin group(200 mg/L),and high concentration baicalin group(200 mg/L)+Broussonin E group(20 μmol/L JAK2/STAT3 activator).Colony formation experiments were applied to detect the proliferation ability of cells in each group; flow cytometry was applied to detect cell apoptosis in each group; Transwell method was applied to detect the invasiveness of cells in each group; ELISA method was applied to detect the levels of interleukin(IL)-18 and IL-1β in cells; Western blot was applied to detect the protein expression of phosphorylated JAK2(p-JAK2)/JAK2,phosphorylated STAT3(p-STAT3)/STAT3,proliferating cell nuclear antigen(PCNA),Bcl-2-associated X protein(BAX),and matrix metalloproteinase-9(MMP-9)of cells in each group.Results:Compared with control group,the colony forming rate,the number of cell invasion,the levels of IL-18,IL-1β,and the protein expressions of p-JAK2/JAK2,p-STAT3/STAT3,PCNA,and MMP-9 in CAL27 cells in the low-concentration baicalin group,the medium-concentration baicalin group,and the high-concentration baicalin group decreased,the apoptosis rate and the protein expression of BAX increased(all P<0.05).The comparison between the medium-concentration baicalin group,the high-concentration baicalin group and the low-concentration baicalin group,and the high-concentration baicalin group and the medium-concentration baicalin group were consistent with the above results(all P<0.05).Compared with the high-concentration baicalin+Broussonin E group and the high-concentration baicalin group,the trends were opposite to the above results(P<0.05).Conclusion:Baicalin may affect the proliferation,apoptosis and invasion of oral squamous cell carcinoma cells by inhibiting JAK2/STAT3 signaling pathway.

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备注/Memo

备注/Memo:
基金项目:山东省中医药科技项目(Q-2022003); 济南市卫生健康委员会中医药科技计划专项课题(2022-中-09)
更新日期/Last Update: 2024-02-05