[1]贾 慧,康 凯,杨 磊.促红细胞生成素对正畸牙复发大鼠牙周组织改建及Wnt/β-catenin信号通路的影响[J].陕西医学杂志,2024,(2):163-167,172.[doi:DOI:10.3969/j.issn.1000-7377.2024.02.004]
　JIA Hui,KANG Kai,YANG Lei.Effects of erythropoietin on periodontal tissue remodeling and Wnt/β-catenin signaling pathway in rats with orthodontic tooth recurrence[J].,2024,(2):163-167,172.[doi:DOI:10.3969/j.issn.1000-7377.2024.02.004]
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促红细胞生成素对正畸牙复发大鼠牙周组织改建及Wnt/β-catenin信号通路的影响
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2024年2期
页码:
163-167,172
栏目:
基础研究
出版日期:
2024-02-05

文章信息/Info

Title:
Effects of erythropoietin on periodontal tissue remodeling and Wnt/β-catenin signaling pathway in rats with orthodontic tooth recurrence
作者:
贾 慧; 康 凯; 杨 磊
(邯郸市口腔医院,河北 邯郸 056001)
Author(s):
JIA Hui; KANG Kai; YANG Lei
(Handan Stomatological Hospital,Handan 056001,China)
关键词:
促红细胞生成素;  正畸牙复发;  Wnt/β-连环蛋白信号通路;  牙周组织改建;  骨质生成;  大鼠
Keywords:
Erythropoietin;  Recurrence of orthodontic teeth;  Wnt/β-catenin signaling pathway;  Periodontal tissue remodeling;  Osteogenesis;  Rats
分类号:
R 783.5
DOI:
DOI:10.3969/j.issn.1000-7377.2024.02.004
文献标志码:
A
摘要:
目的:探究促红细胞生成素对正畸牙复发大鼠牙周组织改建及Wnt/β-连环蛋白(β-catenin)信号通路的影响。方法:构建大鼠正畸牙移动模型,将48只正畸牙模型大鼠随机分为模型组、促红细胞生成素低(250 IU/kg)、高(500 IU/kg)剂量组、促红细胞生成素(500 IU/kg)+KYA1797K(Wnt/β-catenin抑制剂,25 mg/kg)组,每组12只; 分组后拆除各组大鼠加力装置,另取12只健康大鼠作为对照组(不进行任何处理)。各组大鼠给予相应干预30 d。计算取下加力装置第10、20、30天大鼠正畸牙复发率; 苏木素-伊红染色观察牙周组织病理学变化; 免疫组织化学染色检测牙周组织骨形态发生蛋白(BMP)2、BMP4表达; 荧光定量PCR法和蛋白印迹法分别检测牙周组织Wnt、β-catenin mRNA及蛋白表达水平。结果:对照组大鼠牙周组织中纤维排列紧密,细胞整齐分布; 与对照组相比,模型组牙周组织中纤维分散,细胞间排列间隙大,牙槽骨表面有少量成骨细胞排列,没有明显的细胞牙骨质,各时间段正畸牙复发率、牙周组织BMP2、BMP4表达、Wnt、β-catenin mRNA和蛋白水平显著升高(均P<0.05); 与模型组相比,促红细胞生成素低、高剂量组大鼠牙周组织中纤维分布逐渐密集,成骨细胞和细胞牙骨质逐渐增多,各时间段正畸牙复发率依次降低,牙周组织BMP2、BMP4表达、Wnt、β-catenin mRNA和蛋白水平依次升高(均P<0.05); KYA1797K能部分逆转高剂量促红细胞生成素对大鼠正畸牙复发的改善作用(均P<0.05)。结论:促红细胞生成素可能通过激活Wnt/β-catenin信号通路,加速骨质生成,促进牙周组织改建,减缓大鼠正畸牙复发。
Abstract:
Objective:To investigate the effects of erythropoietin on periodontal tissue remodeling and Wnt/β-catenin signaling pathway in rats with orthodontic tooth recurrence.Methods:A rat orthodontic tooth movement model was established,48 orthodontic tooth model rats were randomly divided into model group,erythropoietin low- dose group(250 IU/kg),erythropoietin high-dose group(500 IU/kg),and erythropoietin(500 IU/kg)+KYA1797K(Wnt/β-catenin inhibitor,25 mg/kg)group,12 rats in each group.After grouping,the force device was removed in each group,and another 12 healthy rats were taken as control group(without any treatment).Each group of rats was given corresponding intervention for 30 days.The orthodontic tooth recurrence rate was calculated at 10,20,and 30 days after removing the force device.Hematoxylin-eosin staining was used to observe the pathological changes of periodontal tissue.Immunohistochemical staining was used to detect the expression of bone morphogenetic protein(BMP)2 and BMP4 in the periodontal tissue.The levels of Wnt,β-catenin mRNA and protein in periodontal tissues were detected by fluorescence quantitative PCR and Western blot.Results:The fibers in the periodontal tissue of control group rats are tightly arranged and the cells were evenly distributed.Compared with control group,the fibers in the periodontal tissue of model group were dispersed,the intercellular arrangement space was larger,a few osteoblasts were arranged on the alveolar bone surface,and there was no obvious cellular cementum,the orthodontic tooth recurrence rate in different time periods,the expressions of BMP2 and BMP4,the levels of Wnt,β-catenin mRNA and protein in periodontal tissue significantly increased(all P<0.05).Compared with model group,the fiber distribution in periodontal tissue of rats in erythropoietin low- and high-dose groups was gradually dense,and osteoblasts and cellular cementum were gradually increased,the orthodontic tooth recurrence rate in different time periods were decreased in turn,and the expressions of BMP2,BMP4,the levels of Wnt,β-catenin mRNA and protein in periodontal tissue were increased in turn(all P<0.05).KYA1797K could partially reverse the improvement effect of high dose erythropoietin on orthodontic tooth recurrence in rats(P<0.05).Conclusion:Erythropoietin may accelerate bone formation,promote periodontal tissue remodeling and slow orthodontic tooth recurrence in rats by activating Wnt/β-catenin signaling pathway.

参考文献/References:

[1] 郜罕,王慧敏.无托槽隐形矫治器与固定矫治器对正畸治疗患者龈沟液可溶性细胞间黏附分子-1、白介素-1β、基质金属蛋白酶-8及超氧化物歧化酶、谷胱甘肽过氧化物酶水平的影响[J].陕西医学杂志,2021,50(11):1408-1411,1419.
[2] ALGHAMDI B,JEON H H,NI J,et al.Osteoimmunology in periodontitis and orthodontic tooth movement[J].Curr Osteoporos Rep,2023,21(2):128-146.
[3] KARC L A,BAKA Z M.Assessment of the effects of local platelet-rich fibrin injection and piezocision on orthodontic tooth movement during canine distalization[J].Am J Orthod Dentofacial Orthop,2021,160(1):29-40.
[4] NGUYEN A T.Simulation of orthodontic tooth movement during the activation of an innovative design of closing loop using the finite element method[J].Am J Orthod Dentofacial Orthop,2021,160(2):240-249.
[5] ZHENG X,WANG S,XIAO L,et al.LiCl-induced immunomodulatory periodontal regeneration via the activation of the Wnt/β-catenin signaling pathway[J].J Periodontal Res,2022,57(4):835-848.
[6] 徐翀,申利民,苑文杰.当归多糖通过Wnt/β-catenin信号通路抑制骨关节炎软骨细胞氧化应激损伤与炎性反应[J].陕西中医,2022,43(6):700-703,770.
[7] CHEN J,CUEVAS P L,DWORAN J S,et al.Wnt/β-catenin signaling controls maxillofacial hyperostosis[J].J Dent Res,2022,101(7):793-801.
[8] SERGIO C M,ROLANDO C A.Erythropoietin regulates signaling pathways associated with neuroprotective events[J].Exp Brain Res,2022,240(5):1303-1315.
[9] LIU H,WANG C,SUN X,et al.Silk fibroin/collagen/hydroxyapatite scaffolds obtained by 3D printing technology and loaded with recombinant human erythropoietin in the reconstruction of alveolar bone defects[J].ACS Biomater Sci Eng,2022,8(12):5245-5256.
[10] CHOI J W,KANG S J,CHOI J I,et al.Role of nuclear-receptor-related 1 in the synergistic neuroprotective effect of umbilical cord blood and erythropoietin combination therapy in hypoxic ischemic encephalopathy[J].Int J Mol Sci,2022,23(5):2900-2915.
[11] 代书林,王旭霞,乜福娇,等.异普黄酮对大鼠正畸牙复发过程中牙周组织改建的影响[J].上海口腔医学,2021,30(1):23-27.
[12] 王芹,李立恒,王钟华,等.促红细胞生成素对大鼠磨牙牙髓血运重建术后SDF-1和VEGF表达水平及疼痛的影响[J].河北医学,2022,28(2):224-229.
[13] 王宗康,肖艳波,刘寻,等.加味丹玄口康调控口腔黏膜下纤维化大鼠Wnt/β-catenin信号通路机制研究[J].陕西中医,2022,43(6):687-690,774.
[14] DENG L,CHEN Y,GUO J,et al.Roles and mechanisms of YAP/TAZ in orthodontic tooth movement[J].J Cell Physiol,2021,236(11):7792-7800.
[15] 陈晔,周玉玲,张念.Damon Q自锁托槽正畸治疗疗效及对患者龈沟液氧化应激指标、细胞因子水平的影响[J].陕西医学杂志,2023,52(8):1001-1005.
[16] GRATTON M P,LONDONO I,ROMPRE P,et al.Effect of vitamin D on bone morphometry and stability of orthodontic tooth movement in rats[J].Am J Orthod Dentofacial Orthop,2022,162(6):319-327.
[17] 李雪洋,尹硕,谢金芳,等.促红细胞生成素对大鼠即刻再植牙牙髓血运重建的促进作用[J].吉林大学学报(医学版),2020,46(1):56-60.
[18] TUNHEIM E G,SKALLEVOLD H E,ROKAYA D.Role of hormones in bone remodeling in the craniofacial complex:A review[J].J Oral Biol Craniofac Res,2023,13(2):210-217.
[19] LIN S,YIN S,SHI J,et al.Orchestration of energy metabolism and osteogenesis by Mg2+ facilitates low-dose BMP-2-driven regeneration[J].Bioact Mater,2022,18(1):116-127.
[20] ZUO H,YANG D,WAN Y.Fam 20C regulates bone resorption and breast cancer bone metastasis through osteopontin and BMP4[J].Cancer Res,2021,81(20):5242-5254.
[21] PEREZ-LOZANO M L,SUDRE L,VAN EEGHER S,et al.Gremlin-1 and BMP-4 Overexpressed in osteoarthritis drive an osteochondral-remodeling program in osteoblasts and hypertrophic chondrocytes[J].Int J Mol Sci,2022,23(4):2084-2099.
[22] KUCHLER E C,HANNEGRAF N D,LARA R M,et al.Investigation of genetic polymorphisms in BMP2,BMP4,SMAD6,and RUNX2 and persistent apical periodontitis[J].J Endod,2021,47(2):278-285.
[23] ZHU Z,RUAN S,JIANG Y,et al.α-Klotho released from HK-2 cells inhibits osteogenic differentiation of renal interstitial fibroblasts by inactivating the Wnt-β-catenin pathway[J].Cell Mol Life Sci,2021,78(23):7831-7849.
[24] SHIM N Y,RYU J I,HEO J S.Osteoinductive function of fucoidan on periodontal ligament stem cells:Role of PI3K/Akt and Wnt/β-catenin signaling pathways[J].Oral Dis,2022,28(6):1628-1639.
[25] GUAN X,HE Y,WEI Z,et al.Crosstalk between Wnt/β-catenin signaling and NF-κB signaling contributes to apical periodontitis[J].Int Immunopharmacol,2021,98(1):107843-107857.

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备注/Memo

备注/Memo:
基金项目:河北省医学科学研究计划项目(20200445)
更新日期/Last Update: 2024-02-05