[1]韦 敏,顾春梅,管燕华,等.miR-21对人口腔癌细胞株HSQ-89增殖、侵袭与迁移的影响实验研究[J].陕西医学杂志,2024,(2):157-162.[doi:DOI:10.3969/j.issn.1000-7377.2024.02.003]
　WEI Min,GU Chunmei,GUAN Yanhua,et al.Effect of miR-21 on proliferation,invasion and migration of human oral cancer cell line HSQ-89[J].,2024,(2):157-162.[doi:DOI:10.3969/j.issn.1000-7377.2024.02.003]
点击复制

miR-21对人口腔癌细胞株HSQ-89增殖、侵袭与迁移的影响实验研究
分享到：

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2024年2期
页码:
157-162
栏目:
基础研究
出版日期:
2024-02-05

文章信息/Info

Title:
Effect of miR-21 on proliferation,invasion and migration of human oral cancer cell line HSQ-89
作者:
韦 敏; 顾春梅; 管燕华; 王育新
(南京大学医学院附属口腔医院口腔颌面外科,江苏 南京 210008)
Author(s):
WEI Min; GU Chunmei; GUAN Yanhua; WANG Yuxin
(Department of Oral and Maxillofacial Surgery,Stomatological Hospital Affiliated to Medical School of Nanjing University,Nanjing 210008,China)
关键词:
口腔癌;  微小核糖核酸-21;  转化生长因子β;  细胞增殖;  细胞侵袭;  细胞迁移
Keywords:
Oral cancer;  Micro ribonucleic acid-21;  Transforming growth factor β;  Cell proliferation;  Cell invasion;  Cell migration
分类号:
R 739.8
DOI:
DOI:10.3969/j.issn.1000-7377.2024.02.003
文献标志码:
A
摘要:
目的:探讨微小核糖核酸-21(miR-21)介导Smad7调节转化生长因子β(TGF-β)/Smad信号通路对人口腔癌细胞株HSQ-89增殖、侵袭与迁移的作用。方法:取人口腔癌细胞株HSQ-89培养传代,分为阴性对照组、下调组、上调组和正常组,前三者分别采用脂质体转染法将携带阴性对照(NC inhibitor)、miR-21抑制剂(miR-21 inhibitor)、miR-21模拟物(miR-21 mimics)载体进行转染,正常组仅添加等量无菌蒸馏水。观察各组细胞增殖、侵袭、迁移能力; 实时-定量聚合酶链反应(RT-qPCR)检测各组细胞miR-21、TGF-β1、Smad3、Smad7、细胞周期蛋白D1(CCND1)、MYC、E-钙黏蛋白(E-cadherin)、波形蛋白(Vimentin)、基质金属蛋白酶-2(MMP-2)mRNA表达; 蛋白免疫印迹法(WB)检测各组细胞TGF-β1、Smad3、Smad7、CCND1、MYC、E-cadherin、Vimentin、MMP-2蛋白表达及p-Smad3水平; 双荧光素酶报告基因检测验证miR-21是否靶向Smad7。结果:与正常组和阴性对照组比较,上调组细胞增殖、侵袭、迁移能力上升,下调组细胞增殖活性下降、侵袭细胞数减少、划痕愈合率下降(均P<0.05); 与正常组和阴性对照组比较,上调组细胞miR-21表达、TGF-β1、Smad3、CCND1、MYC、Vimentin、MMP-2 mRNA和蛋白表达上升、Smad7和E-cadherin mRNA和表达下降,差异有统计学意义(均P<0.05),下调组细胞miR-21表达、TGF-β1、Smad3、CCND1、MYC、Vimentin、MMP-2 mRNA和蛋白表达下降、Smad7和E-cadherin mRNA和蛋白表达上升(均P<0.05); 经双荧光素酶报告基因实验验证miR-21靶向Smad7。结论:miR-21促进口腔癌细胞增殖、侵袭和迁移,可能与调节Smad7、TGF-β/Smad信号通路相关。
Abstract:
Objective:To investigate the effect of miR-21 mediating Smad7 to regulate transforming growth factor β(TGF-β)/Smad signaling pathway on the proliferation,invasion and migration of human oral cancer cell lines HSQ-89.Methods:Human oral cancer cell lines HSQ-89 were cultured and subcultured,which was divided into negative control group,down-regulated group,up-regulated group and normal group,The former three were transfected vectors carrying negative control(NC inhibitor),miR-21 inhibitor and miR-21 mimics by liposome transfection method respectively,while the normal group was only added the same amount of sterile distilled water.The cell proliferation,invasive and migration activities were detected.RT-qPCR was used to detect miR-21,TGF-β1,Smad3,Smad7,cyclin D1(CCND1),MYC,E-cadherin,Vimentin and matrix metalloproteinase-2(MMP-2)mRNA expressions in each group.The protein expressions of TGF-β1,Smad3,Smad7,CCND1,MYC,E-cadherin,Vimentin,MMP-2 and p-Smad3 were detected by Western blot(WB).Dual luciferase reporter gene assay was used to verify whether miR-21 targeted Smad7.Results:Compared with the normal group and the negative control group,the cell proliferation,invasion and migration ability of the up-regulated group were increased,and the cell proliferation activity,the number of invasive cells and the scratch healing rate of the down-regulated group were decreased(all P<0.05).Compared with normal group and negative control group,miR-21 expression,mRNA and protein expressions of TGF-β1,Smad3,CCND1,MYC,Vimentin,MMP-2 were increased,while the mRNA and protein expressions of Smad7 and E-cadherin were decreased in up-regulated group(all P<0.05); miR-21 expression,mRNA and protein expressions of TGF-β1,Smad3,CCND1,MYC,Vimentin,MMP-2 were decreased,while the mRNA and protein expressions of Smad7 and E-cadherin were increased in down regulated group(all P<0.05).Dual luciferase reporter gene assay confirmed that miR-21 targeted Smad7.Conclusion:MiR-21 promotes the proliferation,invasion and migration of oral cancer cells,which may be related to regulating Smad7,TGF-β/Smad signaling pathway.

参考文献/References:

[1] YANG Y,ZHOU M,ZENG X,et al.The burden of oral cancer in China,1990-2017:An analysis for the global burden of disease,injuries,and risk factors study 2017[J].BMC Oral Health,2021,21(1):44.
[2] KHOKHAR M A,NIAZ M O,ASLAM A,et al.Pakistan oral cancer collaborative:Analyzing barriers and obstacles to oral cancer diagnosis,treatment,and prevention in Pakistan[J].Oral Surg Oral Med Oral Pathol Oral Radiol,2021,132(3):312-319.
[3] LV S,LUO T,YANG Y,et al.Naa10p and IKKα interaction regulates EMT in oral squamous cell carcinoma via TGF-β1/Smad pathway[J].J Cell Mol Med,2021,25(14):6760-6772.
[4] LIU L,NING S B,FU S,et al.Effects of lncRNA ANRIL on proliferation and apoptosis of oral squamous cell carcinoma cells by regulating TGF-β/Smad pathway[J].Eur Rev Med Pharmacol Sci,2019,23(14):6194-6201.
[5] KUMARASAMY C,MADHAV M R,SABARIMURUGAN S,et al.Prognostic value of mirnas in head and neck cancers:A comprehensive systematic and meta-analysis[J].Cells,2019,8(8):772.
[6] 宋雨红,付启程.基于Wnt/β-catenin通路探究下调miR-21对口腔癌细胞生物学行为的影响[J].临床口腔医学杂志,2020,36(12):712-715.
[7] 李丽,赵龙,冯雯,等.竹节香附素A靶向miR-21-5p/Smad7调控肺腺癌细胞增殖和凋亡的研究[J].四川医学,2021,42(2):131-136.
[8] MCGEARY S E,LIN K S,SHI C Y,et al.The biochemical basis of microRNA targeting efficacy[J].Science,2019,366(6472):1470.
[9] ROI A,ROI C I,ANDREESCU N I,et al.Oral cancer histopathological subtypes in association with risk factors:A 5-year retrospective study[J].Rom J Morphol Embryol,2020,61(4):1213-1220.
[10] 王楠,席锦.口腔癌发病危险因素分析[J].陕西医学杂志,2021,50(4):417-420.
[11] MESIA R,IGLESIAS L,LAMBEA J,et al.SEOM clinical guidelines for the treatment of head and neck cancer(2020)[J].Clin Transl Oncol,2021,23(5):913-921.
[12] 冯瑶.沉默SOCS1基因表达对人口腔癌KB细胞增殖的机制研究[J].陕西医学杂志,2020,49(10):1211-1214.
[13] 王永玲,贾秀改,朱洁,等.粉防己碱调控miR-21表达抑制卵巢癌上皮间质转化的实验研究[J].上海中医药杂志,2020,54(8):71-76.
[14] LIN F,YIN H B,LI X Y,et al.Bladder cancer cell-secreted exosomal miR-21 activates the PI3K/AKT pathway in macrophages to promote cancer progression[J].Int J Oncol,2020,56(1):151-164.
[15] TSE J,PIERCE T,CARLI A L E,et al.Onco-miR-21 promotes Stat3-dependent gastric cancer progression[J].Cancers(Basel),2022,14(2):264.
[16] CHEN C M,CHU T H,CHOU C C,et al.Exosome-derived microRNAs in oral squamous cell carcinomas impact disease prognosis[J].Oral Oncol,2021,120(1):105402.
[17] 张明,蒋兰君,左汴京,等.超声联合血清MYCN、Smad7 mRNA检测对神经母细胞瘤的诊断价值[J].陕西医学杂志,2022,51(11):1441-1444.
[18] ZHANG B,ZHU Z,ZHANG X,et al.Inhibition of the proliferation,invasion,migration,and epithelial-mesenchymal transition of prostate cancer cells through the action of ATP1A2 on the TGF-β/Smad pathway[J].Transl Androl Urol,2022,11(1):53-66.
[19] 刘红艳,齐创.萝卜硫素调控TGF-β1/Smad信号通路抑制结肠癌HT-29细胞增殖的机制研究[J].蚌埠医学院学报,2020,45(3):311-314.
[20] 孙之中,刘朝红,陈其华,等.象皮生肌膏对糖尿病足溃疡大鼠创面组织内上皮-间质转化过程的影响[J].陕西中医,2022,43(12):1664-1668.
[21] THAKUR N,HAMIDI A,SONG J,et al.Smad7 enhances TGF-β-induced transcription of c-Jun and HDAC6 promoting invasion of prostate cancer cells[J].Science,2020,23(9):101470.
[22] 顾海波,孙丽,刘阳祥,等.沉默FMOD对H322细胞的增殖和迁移的影响及其作用机制探讨[J].四川大学学报(医学版),2020,51(2):165-170.
[23] TANG J,LI X,CHENG T,et al.miR-21-5p/SMAD7 axis promotes the progress of lung cancer[J].Thorac Cancer,2021,12(17):2307-2313.
[24] HE Z,LONG J,YANG C,et al.LncRNA DGCR5 plays a tumor-suppressive role in glioma via the miR-21/Smad7 and miR-23a/PTEN axes[J].Aging(Albany NY),2020,12(20):20285-20307.
[25] 冯方方,吴琼,鲁昌辉.清宫排毒汤治疗宫颈癌疗效及对患者肿瘤细胞上皮间质转化的影响[J].陕西中医,2021,42(2):187-191.

相似文献/References:

[1]张树鹰,冯 瑶.沉默SOCS1基因表达对人口腔癌KB细胞增殖的机制研究*[J].陕西医学杂志,2020,49(10):1211.[doi:DOI:10.3969/j.issn.1000-7377.2020.10.003]
　ZHANG Shuying,FENG Yao..Mechanism of silencing SOCS1 gene table on proliferation of human oral cancer KB cells[J].,2020,49(2):1211.[doi:DOI:10.3969/j.issn.1000-7377.2020.10.003]
[2]王 楠,席 锦.口腔癌发病危险因素分析[J].陕西医学杂志,2021,50(4):417.[doi:DOI:10.3969/j.issn.1000-7377.2021.04.008]
　WANG Nan,XI Jin.Analysis of risk factors affecting oral cancer[J].,2021,50(2):417.[doi:DOI:10.3969/j.issn.1000-7377.2021.04.008]
[3]李冬雅,周婷婷,葛殿奎.VEGFR-3、MMP-9、nm23-H1蛋白在口腔癌组织中的表达及其临床价值研究[J].陕西医学杂志,2024,(11):1558.[doi:DOI:10.3969/j.issn.1000-7377.2024.11.026]
　LI Dongya,ZHOU Tingting,GE Diankui.Expression and clinical significance of VEGFR-3,MMP-9 and nm23-H1 proteins in oral cancer tissue[J].,2024,(2):1558.[doi:DOI:10.3969/j.issn.1000-7377.2024.11.026]

备注/Memo

备注/Memo:
基金项目:江苏省重点研发计划(社会发展)专项资金资助项目(BE2021609)
更新日期/Last Update: 2024-02-05