[1]单凤金,张 义,许尧生.丝裂原活化蛋白激酶4、生存素基因在喉鳞状细胞癌组织中的表达及其意义[J].陕西医学杂志,2024,(1):122-126.[doi:DOI:10.3969/j.issn.1000-7377.2024.01.027]
　SHAN Fengjin,ZHANG Yi,XU Yaosheng.Expression and significance of MKK4 and Survivin genes in laryngeal squamous cell carcinoma[J].,2024,(1):122-126.[doi:DOI:10.3969/j.issn.1000-7377.2024.01.027]
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丝裂原活化蛋白激酶4、生存素基因在喉鳞状细胞癌组织中的表达及其意义
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
期数:
2024年1期
页码:
122-126
栏目:
临床病理
出版日期:
2024-01-31

文章信息/Info

Title:
Expression and significance of MKK4 and Survivin genes in laryngeal squamous cell carcinoma
作者:
单凤金; 张 义; 许尧生
(衡水市人民医院耳鼻咽喉头颈外科,河北 衡水 053000)
Author(s):
SHAN Fengjin; ZHANG Yi; XU Yaosheng
(Department of Otorhinolaryngology,Hengshui People's Hospital,Hengshui 053000,China)
关键词:
丝裂原活化蛋白激酶4;  生存素;  喉鳞状细胞癌;  标记物;  预后
Keywords:
Mitogen-activated protein kinase kinase 4;  Survivin;  Laryngeal squamous cell carcinoma;  Marker;  Prognosis
分类号:
R 739.65
DOI:
DOI:10.3969/j.issn.1000-7377.2024.01.027
文献标志码:
A
摘要:
目的:探讨丝裂原活化蛋白激酶4(MKK4)、生存素(Survivin)基因在喉鳞状细胞癌组织中的表达及其意义。方法:选择82例喉癌患为研究对象,取手术切除的喉癌组织及癌旁正常黏膜组织,采用逆转录聚合酶链反应(RT-PCR)法、免疫组织化学法检测MKK4、Survivin基因表达,分析其与临床病理特征的关系,随访记录喉癌患者5年生存率,并用Cox回归模型对喉癌患者预后因素进行分析。结果: 喉癌组织中MKK4 mRNA表达较癌旁组织下降,喉癌组织中Survivin mRNA表达较癌旁组织升高(均P<0.05)。癌旁组织和喉癌组织MKK4蛋白阳性表达率分别为71.95%(59/82)和40.24%(33/82),Survivin 蛋白阳性表达率分别为23.17%(19/82)和71.95%(59/82)(χ2= 16.737,χ2=39.117,均P<0.01)。不同肿瘤分化程度、TNM分期及有无淋巴结转移患者MKK4、Survivin蛋白阳性表达比较,差异有统计学意义(均P<0.05)。喉癌组织中MKK4和 Survivin表达呈负相关(r=-0.403,P<0.05)。MKK4阳性患者5年生存率81.82%(27/33)高于阴性患者65.31%(32/49),Survivin阳性患者5年生存率62.71%(37/59)低于阴性患者82.61%(19/23),影响喉癌患者预后的因素为TNM分期和MKK4、Survivin表达(均P<0.05)。结论:喉癌组织中MKK4表达下调,Survivin表达上调,两者可能是潜在的预后标记物以及治疗靶点。
Abstract:
Objective:To investigate the expression and significance of mitogen-activated protein kinase kinase 4(MKK4)and Survivin in laryngeal squamous cell carcinoma.Methods:Eighty-two cases of laryngeal cancer were selected as the research objects.The expression levels of MKK4 and Survivin in laryngeal cancer tissues and adjacent normal mucosa tissues were detected by RT-PCR and immunohistochemistry,and the relationship between MKK4,Survivin and clinicopathological characteristics was analyzed.The patients were followed up for 5 years,and the survival rate of patients with laryngeal cancer was recorded.Cox proportional hazards regression model was used to analyze the prognostic factors of patients with laryngeal cancer.Results:The expression of MKK4 mRNA in laryngeal carcinoma tissues was lower than that in paracancer tissues,while Survivin mRNA expression was higher in laryngeal carcinoma tissues(all P<0.05).The positive expression rates of MKK4 protein in adjacent tissues and laryngeal cancer tissues were 71.95%(59/82)and 40.24%(33/82),and the positive expression rates of Survivin protein were 23.17%(19/82)and 71.95%(59/82),respectively(χ2=16.737,39.117,all P<0.01).There were significant differences in the expression of MKK4 and Survivin in tumor differentiation,TNM stage,and lymph node metastasis in laryngeal carcinoma tissues(all P<0.05).There was a negative correlation between MKK4 and Survivin expression in laryngeal carcinoma tissues(r=-0.403,P<0.05).The 5-year survival rate of MKK4-positive patients was 81.82%(27/33),higher than 65.31%(32/49)of negative patients(P<0.05).The 5-year survival rate of survivin-positive patients was 62.71%(37/59),lower than 82.61%(19/23)of negative patients(P<0.05).TNM stage,MKK4 and Survivin were independent prognostic factors in patients with laryngeal cancer(all P<0.05).Conclusion:MKK4 expression is down-regulated and Survivin expression is up-regulated in laryngeal carcinoma tissues,which may be potential prognostic markers and therapeutic targets.

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备注/Memo

备注/Memo:
基金项目:河北省医学科学研究计划课题(20210799)
更新日期/Last Update: 2024-01-05