[1]李 伟,王 旭,房清丽.毛细支气管炎患儿糖皮质激素治疗前后血清白细胞介素-6、肿瘤坏死因子-α、微小RNA-302e、核因子-κB mRNA水平变化及意义[J].陕西医学杂志,2023,52(12):1697-1701.[doi:DOI:10.3969/j.issn.1000-7377.2023.12.015]
　LI Wei,WANG Xu,FANG Qingli.Changes of serum IL-6,TNF-α,miR-302E and NF-κB mRNA levels and their significance in children with bronchiolitis before and after glucocorticoid treatment[J].,2023,52(12):1697-1701.[doi:DOI:10.3969/j.issn.1000-7377.2023.12.015]
点击复制

毛细支气管炎患儿糖皮质激素治疗前后血清白细胞介素-6、肿瘤坏死因子-α、微小RNA-302e、核因子-κB mRNA水平变化及意义
分享到：

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年12期
页码:
1697-1701
栏目:
临床研究
出版日期:
2023-12-05

文章信息/Info

Title:
Changes of serum IL-6,TNF-α,miR-302E and NF-κB mRNA levels and their significance in children with bronchiolitis before and after glucocorticoid treatment
作者:
李 伟1; 王 旭2; 房清丽2
(1.大同市第五人民医院儿科,山西 大同 037000; 2.山西大同大学医学院儿科教研组,山西 大同 037000)
Author(s):
LI Wei; WANG Xu; FANG Qingli
(Department of Pediatrics,the Fifth People's Hospital of Datong,Datong 037000,China)
关键词:
毛细支气管炎;  糖皮质激素;  白细胞介素-6;  肿瘤坏死因子-α;  微小RNA-302e;  核因子-κB
Keywords:
Bronchiolitis;  Glucocorticoid;  Interleukin-6;  Tumor necrosis factor-α;  miR-302e;  Nuclear factor-κB
分类号:
R 562.21
DOI:
DOI:10.3969/j.issn.1000-7377.2023.12.015
文献标志码:
A
摘要:
目的:探讨毛细支气管炎患儿糖皮质激素治疗前后血清白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、miR-302e、核因子-κB(NF-κB)mRNA水平变化及意义。方法:选取毛细支气管炎患儿108例为研究对象,均行糖皮质激素治疗。比较糖皮质激素治疗前后患儿血清IL-6、TNF-α、miR-302e、NF-κB mRNA水平变化。采用Pearson法分析IL-6、TNF-α、miR-302e、NF-κB mRNA间的相关性。根据糖皮质激素治疗效果将患儿分为有效组和无效组,比较不同疗效患儿一般资料及生化指标。分析IL-6、TNF-α、miR-302e、NF-κB mRNA水平对糖皮质激素治疗效果的影响,以及它们对毛细支气管炎患儿糖皮质激素治疗无效的预测价值。结果:糖皮质激素治疗后患儿血清IL-6、TNF-α、NF-κB mRNA水平低于治疗前,miR-302e水平高于治疗前(均P<0.05)。Pearson相关性分析显示,患儿血清IL-6与TNF-α、NF-κB mRNA水平呈正相关(r=0.512、0.564,均P<0.05),与miR-302e呈负相关(r=-0.573,P<0.05); 患儿血清TNF-α与NF-κB mRNA水平呈正相关(r=0.521,P<0.05),与miR-302e水平呈负相关(r=-0.559,P<0.05); 患儿血清miR-302e与NF-κB mRNA水平呈负相关(r=-0.571,P<0.05)。无效组患儿血清IL-6、TNF-α、NF-κB mRNA水平,以及重度病情患儿占比高于有效组,miR-302e水平低于有效组(均P<0.05)。Logistic回归分析结果显示,血清IL-6、TNF-α、miR-302e、NF-κB mRNA水平异常以及病情严重程度(重度)是毛细支气管炎患儿糖皮质激素治疗无效的危险因素(均P<0.05)。血清IL-6、TNF-α、miR-302e、NF-κB mRNA联合检测对毛细支气管炎患儿糖皮质激素治疗无效的预测价值高于上述指标单独预测。结论:经糖皮质激素治疗后,毛细支气管炎患儿血清IL-6、TNF-α、NF-κB mRNA水平降低,miR-302e水平升高,四者水平异常是毛细支气管炎患儿糖皮质激素治疗无效的危险因素,联合检测对毛细支气管炎患儿糖皮质激素治疗无效的预测价值更高。
Abstract:
Objective:To investigate the changes of serum interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),miR-302e,nuclear factor-κB(NF-κB)mRNA levels and their significance in children with bronchiolitis before and after glucocorticoid treatment.Methods:108 children with bronchiolitis were selected as the study subjects,all of whom were treated with glucocorticoid.The levels of IL-6,TNF-α,miR-302e and NF-κB mRNA were compared before and after treatment with glucocorticoid.The correlation between IL-6,TNF-α,miR-302e and NF-κB mRNA was analyzed by Pearson method.According to the effect of glucocorticoid treatment,the children were divided into effective group and ineffective group,and the general information and biochemical indexes of the children with different curative effects were compared.The effects of IL-6,TNF-α,miR-302e and NF-κB mRNA levels on clinical efficacy of glucocorticoid therapy in children with bronchiolitis,and the predictive value of failure of glucocorticoid therapy in children with bronchiolitis were investigated.Results:The serum levels of IL-6,TNF-α and NF-κB mRNA in children after glucocorticoid treatment were lower than those before treatment,and the level of miR-302e was higher than that before treatment(all P<0.05).Pearson correlation analysis showed that serum IL-6 was positively correlated with TNF-α and NF-κB mRNA levels(r=0.512,0.564,both P<0.05),and negatively correlated with miR-302e(r=-0.573,P<0.05); serum TNF-α was positively correlated with NF-κB mRNA level(r=0.521,P<0.05),and negatively correlated with miR-302e level(r=-0.559,P<0.05); serum miR-302e was negatively correlated with NF-κB mRNA level(r=-0.571,P<0.05).The levels of serum IL-6,TNF-α,NF-κB mRNA and the proportion of children with severe disease in the ineffective group were higher than those in the effective group,and the level of miR-302e was lower than that in the effective group(all P<0.05).Logistic regression analysis showed that abnormal serum IL-6,TNF-α,miR-302e,NF-κB mRNA levels and severity of the disease were risk factors for failure of glucocorticoid therapy in children with bronchiolitis(all P<0.05).The combined detection of serum IL-6,TNF-α,miR-302e and NF-κB mRNA in children with bronchiolitis has higher predictive value than the above indexes alone.Conclusion:After treatment with glucocorticoids,serum levels of IL-6,TNF-α and NF-κB mRNA decreased and miR-302e level increased in children with bronchiolitis.Abnormal levels of the four are risk factors for the failure of glucocorticoid therapy in children with bronchiolitis.Combined detection has a higher value in predicting the failure of glucocorticoid therapy in children with bronchiolitis.

参考文献/References:

[1] 郑文博,侯 萌.毛细支气管炎患儿血清维生素D水平与免疫功能指标相关性研究[J].陕西医学杂志,2020,49(10):1262-1265.
[2] 潘建丽,王立军,余宏川,等.血清25-OH-D3水平与儿童哮喘急性发作和毛细支气管炎相关性研究[J].陕西医学杂志,2017,46(5):563-564.
[3] 左权杰,张芷铭,舒成仁.布地奈德联合维生素K3注射液治疗小儿毛细支气管炎的效果观察及对炎症因子的影响[J].检验医学与临床,2022,19(4):473-476.
[4] 刘 丹,刘 媛,罗 兰.孟鲁司特与布地奈德吸入对毛细支气管炎患儿的作用及安全性[J].河北医药,2021,43(13):2042-2044.
[5] 王雅宁,崔立业,陈翼霖,等.病毒性毛细支气管炎患儿血清白细胞介素-6、酪氨酸激酶/信号传导及转录激活因子信号通路与免疫功能的关系[J].实用临床医药杂志,2022,26(7):78-81,86.
[6] 侯从岭,芦晓帆,雷小婷,等.异钩藤碱上调miR-192-5p对TNF-α诱导的人支气管上皮细胞凋亡和炎症因子释放的作用及其机制[J].吉林大学学报:医学版,2021,47(3):595-607.
[7] 王 刚,王 琼,姜 昕,等.呼吸道合胞病毒毛细支气管炎婴幼患儿外周血miR-155与PDCD4表达的相关性研究[J].天津医药,2022,50(7):757-761.
[8] Luan Y,Xie B,Wei W.REST-repressed lncRNA NPPA-AS1 regulates cervical cancer progression by modulating miR-302e/DKK1/Wnt/β-catenin signaling pathway[J].J Cell Biochem,2021,122(1):16-28.
[9] 张 续,郑 洁,赵莉平,等.独活寄生汤对膝骨关节炎大鼠IL-17/NF-κB通路的影响[J].陕西中医,2022,43(6):691-695.
[10] 《中华儿科杂志》编辑委员会,中华医学会儿科学分会呼吸学组.毛细支气管炎诊断、治疗与预防专家共识(2014年版)[J].中华儿科杂志,2015,53(3):168-171.
[11] 吴 莹,徐济宝,汪丙松.干扰素联合布地奈德雾化吸入治疗小儿RSV毛细支气管炎的疗效观察[J].医学综述,2021,27(18):3739-3744.
[12] 杨 倩,徐迎军,邹 洋,等.金振口服液联合布地奈德液雾化吸入治疗小儿急性支气管炎的疗效及对肺功能和炎症指标的影响[J].现代生物医学进展,2022,22(10):1956-1959.
[13] 蒋 炜,汤德海,邵家龙.布地奈德联合特布他林雾化吸入治疗小儿毛细支气管炎疗效及其对转录因子FOXP3表达的影响[J].海军医学杂志,2021,42(5):574-577,596.
[14] 刁 敏,张冲林,赵秀侠.重组人干扰素α-2b联合布地奈德、异丙托溴铵雾化对小儿毛细支气管炎疗效及IL-18、IL-33的影响[J].中国细胞生物学学报,2020,42(3):485-490.
[15] 汪 廉,孙明月,彭万胜.毛细支气管炎患儿血清IL-35、Th2类细胞因子水平变化及其意义[J].山东医药,2022,62(3):20-24.
[16] 徐建英,赵 波,张 娟,等.TIMP-1和IL-33水平与儿童RSV感染毛细支气管炎病情严重程度及预后的关系[J].热带医学杂志,2022,22(9):1231-1234,1325.
[17] 吕明珠,陈全景,庞永琴,等.呼吸道合胞病毒感染的毛细支气管炎病儿血清半胱氨酰白三烯、嗜酸性粒细胞阳离子蛋白、免疫球蛋白E及血清炎性因子检测的临床价值[J].安徽医药,2022,26(2):365-368.
[18] 刘秀平,袁 林,苏 伟.小儿支气管炎患者血清25(OH)D3水平与Th17/Treg相关细胞因子的关系[J].中南医学科学杂志,2021,49(6):699-702.
[19] 翟荣娜,白沙沙,熊蕾蕾.肺泡灌洗液高迁移率族蛋白B1和白细胞介素-6诊断儿童迁延性细菌性支气管炎的价值[J].中华医院感染学杂志,2022,32(18):2852-2855.
[20] 蔡东林,孙钟和,赵益庆.慢性阻塞性肺疾病稳定期BODE指数、IL-8、TNF-α水平与肺肾气虚证相关性研究[J].陕西中医,2020,41(11):1573-1575.
[21] Chen B,Song L,Nie X,et al.CXCL1 regulated by miR-302e is involved in cell viability and motility of colorectal cancer via inhibiting JAK-STAT signaling pathway[J].Front Oncol,2021,10:577229.
[22] 刘 晓,胡志德,邓安梅,等.原发性胆汁性肝硬化患者单核细胞内miR-302e表达降低及意义[J].第二军医大学学报,2012,33(2):160-164.
[23] 兰建萍,刘守青,谢欣娥,等.小檗碱对奥氮平诱导胰岛素抵抗大鼠炎症因子及TLR4/NF-κB炎症信号通路的影响[J].实用药物与临床,2021,24(10):887-891.
[24] Li S,Cui W,Song Q,et al.MiRNA-302e attenuates inflammation in infantile pneumonia though the RelA/BRD4/NF-κB signaling pathway[J].Int J Mol Med,2019,44(1):47-56.
[25] 朱占魁,王奇伟,刘鼎立,等.布地奈德联合重组人干扰素α-2b雾化吸入治疗小儿毛细支气管炎临床疗效及安全性分析[J].中华全科医学,2020,18(8):1316-1319,1357.

相似文献/References:

[1]薛佩妮,冯敏娟△,王惠琴.噻托溴铵粉联合糖皮质激素治疗慢性阻塞性肺疾病疗效及对患者生活质量评分的影响*[J].陕西医学杂志,2020,49(2):216.
　XUE Peini,FENG Minjuan,WANG Huiqin..Efficacy of tiotropium bromide combined with glucocorticoids in the treatment of COPD and its effect on patients' quality of life score[J].,2020,49(12):216.
[2]蒋欣宇,李现成,程小红.环孢素A治疗特发性膜性肾病疗效和安全性系统评价Meta分析[J].陕西医学杂志,2021,50(10):1303.[doi:DOI:10.3969/j.issn.1000-7377.2021.10.033]
　JIANG Xinyu,LI Xiancheng,CHENG Xiaohong.Systematic review and meta-analysis of the efficacy and safety of cyclosporine A in treatment of idiopathic membranous nephropathy[J].,2021,50(12):1303.[doi:DOI:10.3969/j.issn.1000-7377.2021.10.033]
[3]陈亚宾,张雪丽,李宏贵,等.嗜酸粒细胞型哮喘患儿外周血单个核细胞肿瘤坏死因子α诱导蛋白8样分子2 mRNA和M2巨噬细胞检测及意义[J].陕西医学杂志,2024,(12):1693.[doi:DOI:10.3969/j.issn.1000-7377.2024.12.022]
　CHEN Yabin,ZHANG Xueli,LI Honggui,et al.Detection and significance of TIPE2 mRNA and M2 macrophages in peripheral blood mononuclear cells of children with eosinophilic asthma[J].,2024,(12):1693.[doi:DOI:10.3969/j.issn.1000-7377.2024.12.022]

备注/Memo

备注/Memo:
基金项目:山西省自然科学研究基金资助面上项目(20210302123306)
更新日期/Last Update: 2023-12-05