[1]曹泰华,刘双爱,赵祖国.宫颈病变患者血清白细胞介素2、白细胞介素4、干扰素γ水平与高危型HPV感染相关性研究[J].陕西医学杂志,2023,52(11):1546-1549,1553.[doi:DOI:10.3969/j.issn.1000-7377.2023.11.020]
　CAO Taihua,LIU Shuang'ai,ZHAO Zuguo.Correlation between serum levels of IL-2,IL-4,IFN-γ and high-risk HPV infection in patients with cervical lesions[J].,2023,52(11):1546-1549,1553.[doi:DOI:10.3969/j.issn.1000-7377.2023.11.020]
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宫颈病变患者血清白细胞介素2、白细胞介素4、干扰素γ水平与高危型HPV感染相关性研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年11期
页码:
1546-1549,1553
栏目:
临床研究
出版日期:
2023-11-05

文章信息/Info

Title:
Correlation between serum levels of IL-2,IL-4,IFN-γ and high-risk HPV infection in patients with cervical lesions
作者:
曹泰华1; 2; 刘双爱2; 赵祖国1
(1.广东医科大学微生物学与免疫学教研室,广东 东莞523808; 2.东莞市妇幼保健院,广东 东莞523000)
Author(s):
CAO Taihua; LIU Shuang'ai; ZHAO Zuguo
(Department of Microbiology and Immunology,Guangdong Medical University,Dongguan 523808,China)
关键词:
高危型人乳头瘤病毒;  白细胞介素2;  白细胞介素4;  干扰素γ;  阴道微环境;  宫颈病变
Keywords:
High-risk human papillomavirus;  Interleukin 2;  Interleukin 4;  Interferon gamma;  Vaginal microenvironment;  Cervical lesions
分类号:
R 711.32
DOI:
DOI:10.3969/j.issn.1000-7377.2023.11.020
文献标志码:
A
摘要:
目的:探讨宫颈病变患者血清白细胞介素2(IL-2)、IL-4、干扰素γ(IFN-γ)水平与高危型人乳头瘤病毒(HR-HPV)感染的相关性。方法:选择126例HR-HPV阳性并经病理确诊上皮类瘤变(CIN)或宫颈癌患者为观察组,另选同期42例正常体检女性为对照组,检测两组患者阴道微环境[乳酸杆菌、需氧菌性阴道炎(AV)、细菌性阴道炎(BV)、外阴阴道假丝酵母菌病(VVC)、滴虫性阴道炎(TV)]情况,酶联免疫吸附法(ELISA)检测两组血清IL-2、IL-4、IFN-γ水平,分析IL-2、IL-4、IFN-γ与HR-HPV感染的关系,并进行HR-HPV感染的多因素Logistic回归分析。结果:CIN Ⅰ级HR-HPV负荷量>1000患者比例低于宫颈癌、CIN Ⅱ级、CIN Ⅲ级患者,HR-HPV负荷量1～100患者比例大于宫颈癌、CIN Ⅱ级、CIN Ⅲ级患者,差异有统计学意义(均P<0.05)。宫颈癌患者乳酸杆菌分级、pH值>4.5、BV、TV、VVC比例高于CIN Ⅰ级患者(均P<0.05)。BV、TV、VVC、乳酸杆菌为HR-HPV 感染的独立危险因素(均P<0.05)。对照组血清IL-2、IFN-γ水平高于CIN Ⅰ级、CIN Ⅱ级、CIN Ⅲ级、宫颈癌患者,IL-4低于CIN Ⅰ级、CIN Ⅱ级、CIN Ⅲ级、宫颈癌患者(均P<0.05)。血清IL-2、IFN-γ与HR-HPV感染呈负相关,IL-4与HR-HPV感染呈正相关(均P<0.01)。结论:HR-HPV感染患者阴道微环境改变,血清IL-2、IFN-γ水平下降,IL-4升高,HR-HPV感染与血清IL-2、IL-4、IFN-γ水平有关,通过检测IL-2、IL-4、IFN-γ水平和阴道局部微环境可能判断CIN的进展情况,并筛选高危人群并密切监测,对减少宫颈癌的发生可能具有重要意义。
Abstract:
Objective:To investigate the correlation between serum levels of interleukin-2(IL-2),IL-4,interferon γ(IFN-γ)and human papillomavirus(HR-HPV)infection in patients with cervical lesions.Methods:126 HR-HPV-positive patients with epithelial neoplasia(CIN)or cervical cancer confirmed by pathology were selected as the observation group,and 42 normal female patients were selected as the control group.The vaginal microenvironment(Lactobacillus,aerobic vaginitis [AV],bacterial vaginitis [BV],vulvovaginal pseudomonas [VVC] and trichomoniasis [TV])of the two groups were detected,and serum levels of IL-2,IL-4 and IFN-γ were detected by enzyme-linked immunosorbent assay(ELISA).The relationship between IL-2,IL-4,IFN-γ and hr-HPV infection was analyzed,and multivariate Logistic regression analysis of HR-HPV infection was performed.Results:The proportion of patients with HR-HPV load > 1000 in CIN Ⅰ was lower than that in cervical cancer,CIN Ⅱ and CIN Ⅲ,while the proportion of patients with HR-HPV load of 1 to 100 was higher than that in cervical cancer,CIN Ⅱ and CIN Ⅲ,with statistical significance(all P<0.05).The lactobacillus grade,pH>4.5,BV,TV,VVC ratio of cervical cancer patients were higher than those of CIN Ⅰ patients(all P<0.05).BV,TV,VVC and lactobacillus were independent risk factors for HR-HPV infection(all P<0.05).The serum levels of IL-2 and IFN-γ in control group were higher than those in CIN Ⅰ,CIN Ⅱ,CIN Ⅲ and cervical cancer patients,and IL-4 was lower than that in CIN Ⅰ,CIN Ⅱ,CIN Ⅲ and cervical cancer patients(all P<0.05).Serum IL-2 and IFN-γ were negatively correlated with HR-HPV infection,while IL-4 was positively correlated with HR-HPV infection(all P<0.01).Conclusion:In patients with HR-HPV infection,vaginal microenvironment changes,serum IL-2 and IFN-γ levels decrease,and IL-4 levels increase.HR-HPV infection is related to serum IL-2,IL-4 and IFN-γ levels,and detection of IL-2,IL-4 and IFN-γ levels and local vaginal microenvironment may determine the progression of CIN.Screening and close monitoring of high-risk groups may be of great significance in reducing the occurrence of cervical cancer.

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备注/Memo

备注/Memo:
基金项目:广东省自然科学基金资助项目(2019MS08204)
更新日期/Last Update: 2023-11-06