[1]滕 鑫,杨正轩,王 媛,等.miR-96-5p靶向PTEN激活PI3K-Akt信号通路抑制非小细胞肺癌细胞增殖、迁移和侵袭实验研究[J].陕西医学杂志,2023,52(11):1511-1516.[doi:DOI:10.3969/j.issn.1000-7377.2023.11.013]
　TENG Xin,YANG Zhengxuan,WANG Yuan,et al.miR-96-5p targeting PTEN promotes non-small cell lung cancer migration,invasion,and proliferation by activating PI3K-Akt pathway[J].,2023,52(11):1511-1516.[doi:DOI:10.3969/j.issn.1000-7377.2023.11.013]
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miR-96-5p靶向PTEN激活PI3K-Akt信号通路抑制非小细胞肺癌细胞增殖、迁移和侵袭实验研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年11期
页码:
1511-1516
栏目:
基础研究
出版日期:
2023-11-05

文章信息/Info

Title:
miR-96-5p targeting PTEN promotes non-small cell lung cancer migration,invasion,and proliferation by activating PI3K-Akt pathway
作者:
滕 鑫; 杨正轩; 王 媛; 倪云峰; 陈德凤; 王 艳; 贠俊茹; 王 娟
(空军军医大学唐都医院,陕西 西安710038)
Author(s):
TENG Xin; YANG Zhengxuan; WANG Yuan; NI Yunfeng; CHEN Defeng; WANG Yan; YUN Junru; WANG Juan
(Tangdu Hospital of Air Force Medical University,Xi'an 710038,China)
关键词:
非小细胞肺癌;  miR-96-5p;  磷酸酯酶与张力蛋白同源物;  细胞迁移;  细胞侵袭;  细胞增殖
Keywords:
Non-small cell lung cancer;  miR-96-5p;  PTEN;  Cell migration;  Cell invasion;  Cell proliferation
分类号:
R 734.2
DOI:
DOI:10.3969/j.issn.1000-7377.2023.11.013
文献标志码:
A
摘要:
目的:探讨miR-96-5p对非小细胞肺癌(NSCLC)细胞迁移、侵袭和增殖的作用及分子机制。方法:在人NSCLC细胞系A549中转染miR-96-5p类似物(miR-96-5p mimics)、阴性对照(NC mimics)或 miR-96-5p抑制剂(miR-96-5p inhibitor)、阴性对照(NC inhibitor),通过CCK-8法检测细胞的增殖能力,通过划痕实验和Transwell迁移实验检测细胞的迁移侵袭能力; 采用荧光素酶报告基因实验检测miR-96-5p与磷酸酯酶与张力蛋白同源物(PTEN)启动子区DNA的结合; 采用蛋白质免疫印迹实验检测miR-96-5p对PTEN/PI3K/Akt信号通路的调控作用。结果:抑制miR-96-5p,NSCLC细胞的迁移、侵袭和增殖能力降低,过表达miR-96-5p促进NSCLC细胞的迁移、侵袭和增殖(均P<0.05)。荧光素酶报告基因实验结果显示,miR-96-5p能与PTEN启动子区DNA结合; 蛋白质免疫印迹实验结果表明,抑制miR-96-5p,PTEN表达升高、p-Akt的表达降低,过表达miR-96-5p后,PTEN表达降低、p-Akt的表达升高(均P<0.05),且沉默PTEN的表达后,逆转了敲低miR-96-5p对p-Akt的抑制作用(均P<0.05)。结论:miR-96-5p通过抑制PTEN,激活PI3K-Akt信号通路,促进NSCLC细胞的迁移、侵袭和增殖。
Abstract:
Objective:To investigate the effect and molecular mechanism of miR-96-5p on the migration,invasion and proliferation of non-small cell lung cancer(NSCLC)cells.Methods:Human NSCLC cell line A549 was transfected with miR-96-5p mimic(miR-96-5p mimics),negative control(NC mimics)or miR-96-5p inhibitor(miR-96-5p inhibitor),negative control(NC inhibitor).The proliferation ability of the cells was detected by CCK-8 method.The migration and invasion ability of the cells were detected by scratch test and Transwell migration test.Luciferase reporter assay was used to detect the binding of miR-96-5p to the promoter region of phosphatase and tensin homolog(PTEN)DNA.Western blot was used to detect the regulatory effect of miR-96-5p on PTEN/PI3K/Akt signaling pathway.Results:Inhibition of miR-96-5p reduced the migration,invasion and proliferation of NSCLC cells,while overexpression of miR-96-5p promoted the migration,invasion and proliferation of NSCLC cells(all P<0.05).Luciferase reporter gene assay showed that miR-96-5p could bind to the DNA of PTEN promoter region.Western blot results showed that inhibition of miR-96-5p increased the expression of PTEN and decreased the expression of p-Akt.After overexpression of miR-96-5p,the expression of PTEN decreased and the expression of p-Akt increased(all P<0.05).The inhibition effect of knockdown of miR-96-5p on p-Akt was reversed(P<0.05).Conclusion:miR-96-5p promotes the migration,invasion and proliferation of NSCLC cells by inhibiting PTEN and activating PI3K-Akt signaling pathway.

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备注/Memo

备注/Memo:
基金项目:陕西省重点研发计划项目(2021SF-102)
更新日期/Last Update: 2023-11-06