[1]党旭升,赵永林,丁 朵,等.芹菜素对大鼠脑外伤的保护作用及机制研究[J].陕西医学杂志,2023,52(11):1458-1462.[doi:DOI:10.3969/j.issn.1000-7377.2023.11.002]
　DANG Xusheng,ZHAO Yonglin,DING Duo,et al.Protective effect and mechanism of apigenin on traumatic brain injury in rats[J].,2023,52(11):1458-1462.[doi:DOI:10.3969/j.issn.1000-7377.2023.11.002]
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芹菜素对大鼠脑外伤的保护作用及机制研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年11期
页码:
1458-1462
栏目:
基础研究
出版日期:
2023-11-05

文章信息/Info

Title:
Protective effect and mechanism of apigenin on traumatic brain injury in rats
作者:
党旭升; 赵永林; 丁 朵; 潘龙飞
(西安交通大学第二附属医院,陕西 西安 710004)
Author(s):
DANG Xusheng; ZHAO Yonglin; DING Duo; PAN Longfei
(The Second Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710004,China)
关键词:
芹菜素;  脑外伤;  血脑屏障;  氧化应激;  炎性因子;  大鼠
Keywords:
Apigenin;  Traumatic brain injury;  Blood brain barrier;  Oxidative stress;  Inflammatory factor;  Rat
分类号:
R 651.15
DOI:
DOI:10.3969/j.issn.1000-7377.2023.11.002
文献标志码:
A
摘要:
目的:探讨芹菜素对大鼠脑外伤(TBI)的保护作用及其机制。方法:72只雄性SD大鼠被随机分为对照组(24只)、TBI组(24只)和TBI+芹菜素组(24只),采用 Feeney 法自由落体打击装置建立大鼠TBI模型,TBI+芹菜素组在造模后每天腹腔注射芹菜素(40 mg/kg),持续3 d。取大鼠伤侧皮层,免疫组化检测神经微丝中链蛋白(NF-M)、胶质纤维酸性蛋白(GFAP)及离子钙接头蛋白抗体-1(Iba-1)的表达; Western blot检测紧密连接相关蛋白Claudin-5、ZO-1、Occludin-1的表达; 伊文斯蓝及脑组织含水量检测血脑屏障通透性情况; ELISA检测炎症因子肿瘤坏死因子(TNF-α)、白细胞介素1β(IL-1β)和白细胞介素6(IL-6)的水平,利用丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)检测氧化应激水平。结果:与对照组相比,TBI组大鼠皮层中NF-M、GFAP、Iba-1、TNF-α、IL-1β、IL-6、MDA的表达均升高(均P<0.05); 伊文斯蓝及脑组织含水量均增加(均P<0.05); Claudin-5、ZO-1、Occludin-1、SOD、CAT及GSH-Px的表达均降低(均P<0.05)。与TBI组相比,TBI+芹菜素组NF-M、GFAP、Iba-1、TNF-α、IL-1β、IL-6、MDA的表达均降低(均P<0.05); 伊文斯蓝及脑组织含水量均减少(均P<0.05); Claudin-5、ZO-1、Occludin-1、SOD、CAT及GSH-Px的表达均升高(均P<0.05)。结论:芹菜素通过抑制胶质细胞活性,降低体内的炎症及氧化应激水平,保护血脑屏障,减轻大鼠TBI后的神经损伤。
Abstract:
Objective:To investigate the protective effect and mechanism of apigenin on traumatic brain injury(TBI)in rats.Methods:72 SD male rats were randomly divided into three groups:the control group(n=24),TBI group(n=24)and TBI+apigenin group(n=24).TBI model was established by Feeney's free-fall device.Rats in TBI+apigenin group were intraperitoneally injected with apigenin(40 mg/kg)every day for consecutive 3 days after modeling.Immunohistochemical staining was used to detect the expression of neurofilament-M(NF-M),glial fibrillary acidic protein(GFAP)and ionized calcium binding adaptor molecule-1(Iba-1).Western blot was used to detect the expressions of Claudin-5、ZO-1、Occludin-1.The permeability of blood brain barrier was detected by Evans blue and brain water content.ELISA was used to detect the levels of inflammatory factors including tumor necrosis factor α(TNF-α),interleukin-1β(IL-1β)and IL-6.Oxidative stress was detected by Superoxide dismutase(SOD),catalaise(CAT),Glutathione peroxidase(GSH-Px)and malondialdehyde(MDA).Results:Compared with the control group,in the TBI group the expressions of NF-M,GFAP,Iba-1,TNF-α,IL-1β,IL-6、MDA were increased,the levels of Evans blue and brain water content were elevated,and the expressions of Claudin-5、ZO-1,Occludin-1,SOD,CAT and GSH-Px were decreased(all P<0.05).Compared with the TBI group,in the TBI+apigenin group,the expressions of NF-M、GFAP、Iba-1、TNF-α、IL-1β、IL-6、MDA were decreased,the levels of Evans blue and brain water content were declined,and the expressions of Claudin-5、ZO-1、Occludin-1、SOD、CAT and GSH-Px were increased(all P<0.05).Conclusion:Apigenin can reduce the levels of inflammation and oxidative stress to protect the blood-brain barrier by inhibiting the activity of glial cells,and reduce the nerve injury after TBI in rats.

参考文献/References:

[1] 闫志丰,陈明生,葛顺楠,等.中型颅脑创伤预后的危险因素分析[J].空军军医大学学报,2022,43(5):432-435,439.
[2] 崔国胜,刘四新,郝有智,等.高压氧联合手厥阴经电刺激干预脑外伤后昏迷患者的临床效果及对GCS评分、生活质量的影响[J].陕西中医,2018,39(1):50-52.
[3] 傅旖灵,聂妍琦,刘孜琦,等.电针抑制脑缺血再灌注损伤大鼠细胞凋亡机制研究[J].陕西中医,2023,44(3):285-289.
[4] Salehi B,Venditti A,Sharifi-Rad M,et al.The therapeutic potential of apigenin[J].Int J Mol Sci,2019,20(6):991-996.
[5] 孙晓力,翟宏军,张心武,等.芹菜素诱导人乳腺癌MDA-MB-231细胞凋亡的实验研究[J].陕西医学杂志,2017,46(10):1335-1337,1365.
[6] Kuru BP,Demir D,Koyuncuoglu T,et al.Possible anti-inflammatory,antioxidant,and neuroprotective effects of apigenin in the setting of mild traumatic brain injury:An investigation[J].Immunopharmacol Immunotoxicol,2023,45(2):185-196.
[7] Chen X,Chen C,Fan S,et al.Omega-3 polyunsaturated fatty acid attenuates the inflammatory response by modulating microglia polarization through SIRT1-mediated deacetylation of the HMGB1/NF-κB pathway following experimental traumatic brain injury[J].J Neuroinflammation,2018,15(1):116.
[8] Zhao Y,Zhao J,Zhang M,et al.Involvement of toll like receptor 2 signaling in secondary injury during experimental diffuse axonal injury in rats[J].Mediators Inflamm,2017,2017:1570917.
[9] Khellaf A,Khan DZ,Helmy A.Recent advances in traumatic brain injury[J].J Neurol,2019,266(11):2878-2889.
[10] Singh D,Gupta M,Sarwat M,et al.Apigenin in cancer prevention and therapy:A systematic review and meta-analysis of animal models[J].Crit Rev Oncol Hematol,2022,176:103751.
[11] Wang X,Li J,Zhao D,et al.Therapeutic and preventive effects of apigenin in cerebral ischemia:A review[J].Food Funct,2022,13(22):11425-11437.
[12] Xu Y,Li X,Wang H.Protective roles of apigenin against cardiometabolic diseases:A systematic review[J].Front Nutr,2022,9:875826.
[13] Siddique YH,Rahul,Ara G,et al.Beneficial effects of apigenin on the transgenic drosophila model of Alzheimer's disease[J].Chem Biol Interact,2022,366:110120.
[14] Bijani S,Dizaji R,Sharafi A,et al.Neuroprotective effect of apigenin on depressive-like behavior:Mechanistic approach[J].Neurochem Res,2022,47(3):644-655.
[15] Ren B,Qin W,Wu F,et al.Apigenin and naringenin regulate glucose and lipid metabolism,and ameliorate vascular dysfunction in type 2 diabetic rats[J].Eur J Pharmacol,2016,773:13-23.
[16] Jha RM,Kochanek PM,Simard JM.Pathophysiology and treatment of cerebral edema in traumatic brain injury[J].Neuropharmacology,2019,145(2):230-246.
[17] Cash A,Theus MH.Mechanisms of blood-brain barrier dysfunction in traumatic brain injury[J].Int J Mol Sci,2020,21(9):1451-1358.
[18] Wu Q,Li W,Zhao J,et al.Apigenin ameliorates doxorubicin-induced renal injury via inhibition of oxidative stress and inflammation[J].Biomed Pharmacother,2021,137:111308.
[19] Zheng S,Cao P,Yin Z,et al.Apigenin protects mice against 3,5-diethoxycarbonyl-1,4-dihydrocollidine-induced cholestasis[J].Food Funct,2021,12(5):2323-2334.
[20] 史文哲,罗文哲,宋汉君,等.大鼠脑外伤后星形胶质细胞及血清中相关蛋白随时间表达变化研究[J].陕西医学杂志,2022,51(8):934-938.

备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(82001327)
更新日期/Last Update: 2023-11-06