[1]李 杰,李 瑞,李虹良,等.槲皮素通过生长抑制特异性基因5调节微小RNA-23a-3p和磷脂酰肌醇3激酶/蛋白激酶B信号通路抑制垂体神经内分泌肿瘤的增殖机制[J].陕西医学杂志,2023,52(10):1313-1318.[doi:DOI:10.3969/j.issn.1000-7377.2023.10.007]
　LI Jie,LI Rui,LI Hongliang,et al.Quercetin inhibits the proliferation of pituitary neuroendocrine tumors by regulating miR-23a-3p and PI3K/AKT signaling pathway through GAS5[J].,2023,52(10):1313-1318.[doi:DOI:10.3969/j.issn.1000-7377.2023.10.007]
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槲皮素通过生长抑制特异性基因5调节微小RNA-23a-3p和磷脂酰肌醇3激酶/蛋白激酶B信号通路抑制垂体神经内分泌肿瘤的增殖机制
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年10期
页码:
1313-1318
栏目:
基础研究
出版日期:
2023-10-05

文章信息/Info

Title:
Quercetin inhibits the proliferation of pituitary neuroendocrine tumors by regulating miR-23a-3p and PI3K/AKT signaling pathway through GAS5
作者:
李 杰; 李 瑞; 李虹良; 陈康雪; 孙中磊
(北京精诚博爱医院神经二科,北京 100015)
Author(s):
LI Jie; LI Rui; LI Hongliang; CHEN Kangxue; SUN Zhonglei
(The Second Department of Neurology,Beijing Jingcheng Boai Hospital,Beijing 100015,China)
关键词:
槲皮素;  垂体神经内分泌肿瘤;  人垂体瘤细胞系;  生长抑制特异性基因5;  微小RNA-23a-3p;  脂酰肌醇3激酶/蛋白激酶B信号通路
Keywords:
Quercetin;  Pituitary neuroendocrine tumors;  Human pituitary tumor cell line;  Growth inhibition specific gene 5;  miR-23a-3p;  PI3K/AKT signaling pathway
分类号:
R 736.4
DOI:
DOI:10.3969/j.issn.1000-7377.2023.10.007
文献标志码:
A
摘要:
目的:探讨槲皮素对垂体神经内分泌肿瘤(PitNETs)生长的抑制作用及其可能机制。方法:通过Starbase工具预测生长抑制特异性基因5(GAS5)与微小RNA-23a-3p(miR-23a-3p)结合情况,双荧光素酶报告实验和RNA免疫沉淀测定GAS5和miR-23a-3p之间的结合关系。体外培养人垂体瘤细胞系RC-4B/C,分为对照组(Control)、槲皮素组(Quercetin)、GAS5抑制组(Si-GAS5)和槲皮素+si-GAS5组(Quercetin+si-GAS5),对比分析槲皮素对GAS5、miR-23a-3p及磷脂酰肌醇3激酶(PI3K)/蛋白激酶 B(AKT)信号通路的影响,以及人垂体瘤复苏细胞(RC-4B/C)生长和侵袭能力的影响。结果: Starbase工具预测GAS5与miR-23a-3p 结合,双荧光素酶报告实验和RNA免疫沉淀测定揭示了GAS5和miR-23a-3p之间的直接结合。槲皮素促进 GAS5的表达,抑制miR-23a-3p和PI3K/AKT的表达,抑制RC-4B/C细胞的增殖和侵袭(均P<0.05); 下调GAS5、miR-23a-3p表达增多,促进RC-4B/C细胞的增殖和侵袭(均P<0.05); 槲皮素可以逆转GAS5 下调引起的miR-23a-3p、PI3K/AKT表达增加,抑制RC-4B/C细胞的增殖和侵袭(均P<0.05)。结论:槲皮素可能通过促进GAS5表达竞争性抑制miR-23a-3p,降低了PI3K/AKT,抑制垂体神经内分泌肿瘤的生长。
Abstract:
Objective:To investigate the inhibitory effect of quercetin on the growth of pituitary neuroendocrine tumors and its possible mechanism.Methods:Starbase tool was used to predict the combination of growth arrest-specific transcripts(GAS5)with miR-23a-3p.Dual luciferase assay and RNA immunoprecipitation were used to determine the binding relationship between GAS5 and miR-23a-3p.Human pituitary tumor cell line RC-4B/C was cultured in vitro and divided into control group(control group),quercetin group(quercetin group),GAS5 inhibition group(si-GAS5 group)and quercetin+si-GAS5 group.The effects of quercetin on GAS5,miR-23a-3p and PI3K/AKT signaling pathway,as well as the effects on the growth and invasion ability of RC-4B/C tumor cells were compared and analyzed.Results:The Starbase tool predicted GAS5 binding to miR-23a-3p,and dual luciferase reporting assay and RNA immunoprecipitation assay revealed the direct binding between GAS5 and miR-23a-3p.Quercetin promoted the expression of GAS5,inhibited the expression of miR-23a-3p and PI3K/AKT,and inhibited the proliferation and invasion of RC-4B/C cells(P<0.05).Down-regulated GAS5 and increased miR-23a-3p expression promoted the proliferation and invasion of RC-4B/C cells(all P<0.05).Quercetin could reverse the increased expression of miR-23a-3p and PI3K/AKT caused by GAS5 downregulation,and inhibit the proliferation and invasion of RC-4B/C cells(all P<0.05).Conclusion:Quercetin may competitively inhibit miR-23a-3p by promoting GAS5 expression,reduce PI3K/AKT,and inhibit the growth of pituitary neuroendocrine tumors.

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备注/Memo

备注/Memo:
基金项目:国家重点研发计划项目(2016YFC1101503)
更新日期/Last Update: 2023-10-07