[1]陈 萍,马小平,蔡 爽,等.过表达叉头盒F1对肝癌细胞增殖、周期和凋亡的影响及机制实验研究[J].陕西医学杂志,2023,52(9):1145-1149.[doi:DOI:10.3969/j.issn.1000-7377.2023.09.007]
　CHEN Ping,MA Xiaoping,CAI Shuang,et al.Effects of FOXF1 overexpression on proliferation,cycle and apoptosis of hepatocellular carcinoma cells and its mechanism[J].,2023,52(9):1145-1149.[doi:DOI:10.3969/j.issn.1000-7377.2023.09.007]
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过表达叉头盒F1对肝癌细胞增殖、周期和凋亡的影响及机制实验研究

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年9期
页码:
1145-1149
栏目:
基础研究
出版日期:
2023-09-05

文章信息/Info

Title:
Effects of FOXF1 overexpression on proliferation,cycle and apoptosis of hepatocellular carcinoma cells and its mechanism
作者:
陈 萍1; 马小平2; 蔡 爽2; 贾浴侦2; 李仕伟2
(1.天水市第一人民医院老年病科,甘肃 天水 741000; 2.西安交通大学医学部基础医学院细胞生物学与遗传学系,陕西 西安 710061)
Author(s):
CHEN Ping; MA Xiaoping; CAI Shuang; JIA Yuzhen; LI Shiwei
(Department of Geriatrics,the First People's Hospital of Tianshui,Tianshui 741000,China)
关键词:
肝癌细胞;  叉头盒F1;  细胞增殖;  细胞凋亡;  细胞周期;  Wnt5a/β-catenin通路
Keywords:
Hepatoma carcinoma cell;  Forkhead box F1;  Cell proliferation;  Cell apoptosis;  Cell cycle;  Wnt5a/β-catenin pathway
分类号:
R 735.7
DOI:
DOI:10.3969/j.issn.1000-7377.2023.09.007
文献标志码:
A
摘要:
目的:探讨过表达叉头盒F1(FOXF1)对肝癌细胞增殖、周期和凋亡的影响,并初步探究其分子机制。方法:选取肝癌细胞(MHCC-97H和Huh7细胞)为实验细胞,分别设立vector组(转染空白载体)和ov-FOXF1组(转染FOXF1过表达载体)。采用实时荧光定量PCR(RT-qPCR)和Western bolt检测转染后FOXF1 mRNA及蛋白表达。采用MTT法检测肝癌细胞增殖。采用流式细胞术检测肝癌细胞周期和细胞凋亡。采用Western bolt检测Wnt5a/β-catenin信号通路相关蛋白表达。结果:ov-FOXF1组Huh7和MHCC-97H细胞FOXF1 mRNA和蛋白表达量高于vector组(均P<0.05)。转染后48、72 h,ov-FOXF1组Huh7和MHCC-97H细胞增殖受到抑制(均P<0.05)。与vector组比较,ov-FOXF1组Huh7和MHCC-97H细胞G0/G1期细胞比例升高,S期细胞比例降低(均P<0.05)。ov-FOXF1组G2/M期Huh7细胞比例较vector组降低(P<0.05)。MHCC-97H细胞两组G2/M期细胞比例比较差异无统计学意义(均P>0.05)。ov-FOXF1组Huh7和MHCC-97H细胞凋亡比例高于vector组(均P<0.05)。与vector组比较,ov-FOXF1组FOXF1、active Caspase-3蛋白表达增加,Wnt5a、β-catenin、c-myc、细胞周期蛋白D1(Cyclin D1)蛋白表达降低(均P<0.05)。结论:FOXF1过表达能够抑制肝癌细胞的增殖及周期进展,促进细胞凋亡,其机制可能与调控Wnt5a/β-catenin通路及细胞周期相关蛋白等有关。
Abstract:
Objective:To investigate the effects of forkhead box F1(FOXF1)overexpression on the proliferation,cycle and apoptosis of hepatocellular carcinoma cells,and to explore its molecular mechanism.Methods:Hepatocellular carcinoma cells(MHCC-97H and Huh7 cells)were selected as experimental cells,and vector group(transfected with blank vector)and ov-FOXF1 group(transfected with FOXF1 overexpression vector)were set up,respectively.RT-qPCR and Western blot were used to detect the expressions of FOXF1 mRNA and protein after transfection.The proliferation of hepatocellular carcinoma cells was detected by MTT assay.The cell cycle and apoptosis of hepatocellular carcinoma cells were detected by flow cytometry.Western blot was used to detect the expression of Wnt5a/β-catenin signaling pathway-related proteins.Results:The expressions of FOXF1 mRNA and protein in Huh7 and MHCC-97H cells in ov-FOXF1 group were higher than those in vector group(all P<0.05).At 48 and 72 hours after transfection,the proliferation of Huh7 and MHCC-97H cells in ov-FOXF1 group was inhibited(both P<0.05).Compared with the vector group,the proportion of G1/G0 phase cells in Huh7 and MHCC-97H cells in ov-FOXF1 group increased,and the proportion of S phase cells decreased(all P<0.05).Compared with the vector group,the ov-FOXF1 group had a significantly lower proportion of Huh7 cells in G2/M phase(P<0.05).There was no significant difference in the proportion of G2/M phase cells between the two groups of MHCC-97H cells(all P>0.05).The apoptosis rate of Huh7 and MHCC-97H cells in ov-FOXF1 group was higher than that in vector group(both P<0.05).Compared with the vector group,the expressions of FOXF1 and active Caspase-3 proteins in the ov-FOXF1 group were increased,and the expressions of Wnt5a,β-catenin,c-myc and Cyclin D1 proteins were decreased(all P<0.05).Conclusion:The overexpression of FOXF1 can inhibit the proliferation and cycle progression of hepatocellular carcinoma cells,and promote cell apoptosis.The mechanism may be related to the regulation of Wnt5a/β-catenin pathway and cell cycle-related proteins.

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备注/Memo

备注/Memo:
基金项目:陕西省自然科学基础研究计划项目(2020JM-074)
更新日期/Last Update: 2023-09-04