[1]依力哈木·买买提,钟 锴,艾合散·卡马力,等.环状RNA circ_0020123靶向调控微小RNA-145影响胰腺癌细胞SW-1990增殖、侵袭与转移实验研究[J].陕西医学杂志,2023,52(9):1140-1144.[doi:DOI:10.3969/j.issn.1000-7377.2023.09.006]
　YILIHAMU Maimaiti,ZHONG Kai,AIHESAN Kamali,et al.Circular RNA circ_0020123 affects proliferation,invasion and metastasis of pancreatic cancer cell SW-1990 by targeting microRNA-145[J].,2023,52(9):1140-1144.[doi:DOI:10.3969/j.issn.1000-7377.2023.09.006]
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环状RNA circ_0020123靶向调控微小RNA-145影响胰腺癌细胞SW-1990增殖、侵袭与转移实验研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年9期
页码:
1140-1144
栏目:
基础研究
出版日期:
2023-09-05

文章信息/Info

Title:
Circular RNA circ_0020123 affects proliferation,invasion and metastasis of pancreatic cancer cell SW-1990 by targeting microRNA-145
作者:
依力哈木·买买提1; 钟 锴1; 艾合散·卡马力1; 杨 鹏1; 依马木买买提江·阿布拉2
(1.新疆医科大学第一附属医院,新疆 乌鲁木齐 830054; 2.新疆医科大学第三临床医学院肝胆胰外科,新疆 乌鲁木齐 830011)
Author(s):
YILIHAMU Maimaiti; ZHONG Kai; AIHESAN Kamali; YANG Peng; YIMAMUMAIMAITIJIANG Abula
(the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China)
关键词:
胰腺癌;  环状RNA;  circ_0020123;  微小RNA-145;  增殖;  侵袭;  转移
Keywords:
Pancreatic cancer;  Circular RNA;  Circ_0020123;  miR-145;  Proliferation;  Invasion;  Metastasis
分类号:
R 735.9
DOI:
DOI:10.3969/j.issn.1000-7377.2023.09.006
文献标志码:
A
摘要:
目的:探讨环状RNA circ_0020123靶向调控微小RNA(miR)-145对胰腺癌细胞SW-1990增殖、侵袭与转移的影响。方法:选择胰腺癌细胞株SW-1990,分为si-NC组、si-circ_0020123组、si-circ_0020123+anti-miR-145组,进行细胞转染。采用MTT法检测细胞增殖能力。采用Transwell侵袭实验检测细胞侵袭与迁移能力。采用反转录定量聚合酶链反应(RT-qPCR)检测circ_0020123和miR-145相对表达量。采用Western blot检测基质金属蛋白酶-9(MMP-9)、B淋巴细胞瘤-2(Bcl-2)、磷酸化腺苷酸活化蛋白激酶(p-AMPK)。采用双荧光素酶报告实验分析circ_0020123与miR-145的靶向作用。结果:与si-NC组比较,si-circ_0020123组和si-circ_0020123+anti-miR-145组细胞增殖、侵袭及迁移能力明显降低(均P<0.05)。与si-circ_0020123组比较,si-circ_0020123+anti-miR-145组细胞增殖、侵袭及迁移能力明显升高(均P<0.05)。与si-NC组比较,si-circ_0020123组和si-circ_0020123+anti-miR-145组细胞凋亡率升高(均P<0.05)。与si-circ_0020123组比较,si-circ_0020123+anti-miR-145组细胞凋亡率明显降低(P<0.05)。与miR-NC组比较,miR-145组circ_0020123-WT荧光素酶活性降低(P<0.05)。与si-NC组比较,si-circ_0020123组和si-circ_0020123+anti-miR-145组细胞MMP-9、Bcl-2表达水平降低,p-AMPK表达水平升高(均P<0.05)。与si-circ_0020123组比较,si-circ_0020123+anti-miR-145组细胞MMP-9、Bcl-2表达水平升高,p-AMPK表达水平降低(均P<0.05)。结论:circ_0020123通过靶向miR-145可抑制胰腺癌细胞的增殖、侵袭与转移,而沉默miR-145可逆转上述作用。
Abstract:
Objective:To investigate the effects of circular RNA circ_0020123 on the proliferation,invasion and metastasis of pancreatic cancer cell SW-1990 by targeting microrna(miR)-145.Methods:Pancreatic cancer cell line SW-1990 was selected and divided into si-NC group,si-circ_0020123 group and si-circ_0020123+anti-miR-145 group for cell transfection.MTT assay was used to detect cell proliferation.Transwell invasion assay was used to detect cell invasion and migration.RT-qPCR was used to detect the relative expression of circ_0020123 and miR-145.MMP-9,Bcl-2 and p-AMPK were detected by Western blot.The dual luciferase reporter experiment was used to analyze the targeting effect of circ_0020123 and miR-145.Results:Compared with the si-NC group,the cell proliferation,invasion and migration ability of the si-circ_0020123 group and the si-circ_0020123+anti-miR-145 group were significantly reduced(all P<0.05).Compared with the si-circ_0020123 group,the cell proliferation,invasion and migration ability of the si-circ_0020123+anti-miR-145 group were significantly increased(all P<0.05).Compared with the si-NC group,the apoptosis rates of the si-circ_0020123 group and the si-circ_0020123+anti-miR-145 group were increased(all P<0.05).Compared with the si-circ_0020123 group,the apoptosis rate of the si-circ_0020123+anti-miR-145 group was significantly decreased(P<0.05).Compared with the miR-NC group,the circ_0020123-WT luciferase activity in the miR-145 group was decreased(P<0.05).Compared with the si-NC group,the expression levels of MMP-9 and Bcl-2 in the si-circ_0020123 group and the si-circ_0020123+anti-miR-145 group were decreased,and the expression level of p-AMPK was increased(all P<0.05).Compared with the si-circ_0020123 group,the expression levels of MMP-9 and Bcl-2 in the si-circ_0020123+anti-miR-145 group were increased,and the expression level of p-AMPK was decreased(all P<0.05).Conclusion: Circ_0020123 can inhibit the proliferation,invasion and metastasis of pancreatic cancer cells by targeting miR-145,while silencing miR-145 can reverse the above effects.

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备注/Memo

备注/Memo:
基金项目:新疆维吾尔自治区自然科学基金资助项目(2022D01C292)
更新日期/Last Update: 2023-09-04