[1]刘 俊,曾 龙,高 云,等.长链非编码RNA YAF2-AS1通过调控微小RNA-141-3p表达抑制肝星状细胞活化实验研究[J].陕西医学杂志,2023,52(9):1120-1124.[doi:DOI:10.3969/j.issn.1000-7377.2023.09.002]
　LIU Jun,ZENG Long,GAO Yun,et al.Long-chain non-coding RNA YAF2-AS1 inhibits activation of hepatic stellate cells by regulating expression of microRNA-141-3p[J].,2023,52(9):1120-1124.[doi:DOI:10.3969/j.issn.1000-7377.2023.09.002]
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长链非编码RNA YAF2-AS1通过调控微小RNA-141-3p表达抑制肝星状细胞活化实验研究

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年9期
页码:
1120-1124
栏目:
基础研究
出版日期:
2023-09-05

文章信息/Info

Title:
Long-chain non-coding RNA YAF2-AS1 inhibits activation of hepatic stellate cells by regulating expression of microRNA-141-3p
作者:
刘 俊1; 曾 龙1; 高 云1; 敖会芳2; 林 勇3; 魏雪源1
(1.荆门市人民医院 荆楚理工学院附属中心医院感染性疾病科,湖北 荆门 448000; 2.荆门市人民医院 荆楚理工学院附属中心医院血液内科,湖北 荆门 448000; 3.上海长征医院消化内科,上海 200003)
Author(s):
LIU Jun; ZENG Long; GAO Yun; AO Huifang; LIN Yong; WEI Xueyuan
(Department of Infectious Diseases,Jingmen People's Hospital,Jingmen 448000,China)
关键词:
肝纤维化;  长链非编码RNA;  YAF2-AS1;  微小RNA-141-3p;  肝星状细胞;  增殖
Keywords:
Liver fibrosis;  Long non-coding RNA;  YAF2-AS1;  miR-141-3p;  Hepatic stellate cells;  Proliferation
分类号:
R 575.2
DOI:
DOI:10.3969/j.issn.1000-7377.2023.09.002
文献标志码:
A
摘要:
目的:探讨长链非编码RNA(lncRNA)YAF2-AS1在肝星状细胞活化中的作用及可能的分子机制。方法:采用基因表达数据库(GEO)分析YAF2-AS1在肝纤维化组织和正常肝组织中的表达。用YAF2-AS1质粒转染人肝星状细胞LX-2细胞并设为YAF2-AS1组,以转染阴性对照质粒为对照组。采用CCK-8实验检测LX-2细胞增殖。采用流式细胞术检测LX-2细胞周期分布和活性氧(ROS)含量。采用lncRMap软件和双荧光素酶活性实验验证YAF2-AS1与miR-141-3p的靶向关系。采用实时荧光定量聚合酶链反应(qRT-PCR)检测miR-141-3p表达。采用Western blot检测蛋白磷酸酶及张力蛋白同源物(PTEN)/蛋白激酶B(AKT)信号通路中PTEN、p-AKT蛋白以及肝纤维化标志物[α平滑肌肌动蛋白(α-SMA)、Ⅰ型胶原蛋白(Collagen Ⅰ)、转化生长因子β受体Ⅱ(TGFβR2)]的表达。结果:与正常肝组织比较,肝纤维化组织YAF2-AS1表达水平降低(P<0.01)。与对照组比较,YAF2-AS1组LX-2细胞增殖活性被抑制(P<0.05); G0/G1期LX-2细胞比例升高(P<0.01),S期和G2/M期LX-2细胞比例降低(均P<0.05); LX-2细胞ROS含量升高(P<0.01)。miR-141-3p是YAF2-AS1的靶基因(P<0.01)。与对照组比较,YAF2-AS1组LX-2细胞miR-141-3p表达降低(P<0.01); PTEN蛋白表达增加,p-AKT、α-SMA、Collagen Ⅰ、TGFβR2蛋白表达降低(均P<0.05)。结论:YAF2-AS1在肝纤维化组织中表达下调,过表达YAF2-AS1通过降低miR-141-3p表达抑制肝星状细胞的活化。
Abstract:
Objective:To explore the role and possible molecular mechanism of long non-coding RNA(lncRNA)YAF2-AS1 in the activation of hepatic stellate cells. Methods: GEO database was used to analyze the expression of YAF2-AS1 in liver fibrosis tissue and normal liver tissue.Human hepatic stellate cell LX-2 cells were transfected with YAF2-AS1 plasmid and set as YAF2-AS1 group,and the negative control plasmid was used as the control group.CCK-8 assay was used to detect the proliferation of LX-2 cells.The cycle distribution and ROS content of LX-2 cells were detected by flow cytometry.LncRMap software and dual luciferase activity experiment were used to verify the targeting relationship between YAF2-AS1 and miR-141-3p.The expression of miR-141-3p was detected by qRT-PCR.Western blot was used to detect the expression of PTEN/AKT signaling pathway proteins(PTEN and p-AKT)and liver fibrosis markers(α-SMA, Collagen Ⅰ and TGFβR2). Results: Compared with normal liver tissue,the expression level of YAF2-AS1 in liver fibrosis tissue was decreased(P<0.01).Compared with control group,the proliferation activity of LX-2 cells in the YAF2-AS1 group was inhibited(P<0.05); the proportion of LX-2 cells in G0/G1 phase was increased(P<0.01),and the LX-2 cells in S and G2/M phase were decreased(all P<0.05); ROS content of LX-2 cells increased(P<0.01).MiR-141-3p was the target gene of YAF2-AS1(P<0.01).Compared with control group,the expression of miR-141-3p in LX-2 cells in YAF2-AS1 group was decreased(P<0.01); the protein expression of PTEN was increased,and the protein expressions of p-AKT,α-SMA,CollagenⅠ and TGFβR2 were decreased(all P<0.05). Conclusion:The expression of YAF2-AS1 is down-regulated in liver fibrosis tissue,and the overexpression of YAF2-AS1 inhibits the activation of hepatic stellate cells by reducing the expression of miR-141-3p.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金资助项目(81870416)
更新日期/Last Update: 2023-09-04