[1]柏 慧,王 卓,关有良,等.吡咯啉-5-羧酸还原酶-1、增殖细胞核抗原和半胱氨酸天冬氨酸特异性蛋白酶-3在膀胱尿路上皮癌中的表达及检测临床意义[J].陕西医学杂志,2023,52(8):1097-1101.[doi:DOI:10.3969/j.issn.1000-7377.2023.08.036]
　BAI Hui,WANG Zhuo,GUAN Youliang,et al.Expression and significance of PYCR1,PCNA and Caspase-3 in uroepithelial carcinoma of bladder[J].,2023,52(8):1097-1101.[doi:DOI:10.3969/j.issn.1000-7377.2023.08.036]
点击复制

吡咯啉-5-羧酸还原酶-1、增殖细胞核抗原和半胱氨酸天冬氨酸特异性蛋白酶-3在膀胱尿路上皮癌中的表达及检测临床意义
分享到：

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年8期
页码:
1097-1101
栏目:
临床病理
出版日期:
2023-08-05

文章信息/Info

Title:
Expression and significance of PYCR1,PCNA and Caspase-3 in uroepithelial carcinoma of bladder
作者:
柏 慧1; 王 卓2; 关有良1; 李 良2
(1.锦州医科大学附属第一医院,辽宁 锦州121000; 2.锦州医科大学附属第三医院,辽宁 锦州121000)
Author(s):
BAI Hui; WANG Zhuo; GUAN Youliang; LI Liang
(The First Affiliated Hospital of Jinzhou Medical University,Jinzhou 121000,China)
关键词:
膀胱尿路上皮癌;  吡咯啉-5-羧酸还原酶1;  细胞增殖;  细胞凋亡;  预后;  临床特征
Keywords:
Urothelial carcinoma of bladder;  Pyrrolin-5-carpoxylate reductase 1;  Cell proliferation;  Apoptosis;  Prognosis;  Clinical characteristic
分类号:
R 737.14
DOI:
DOI:10.3969/j.issn.1000-7377.2023.08.036
文献标志码:
A
摘要:
目的:观察膀胱尿路上皮癌中吡咯啉-5-羧酸还原酶1(PYCR1)、增殖细胞核抗原(PCNA)和半胱氨酸天冬氨酸特异性蛋白酶-3(Caspase-3)的表达特征。方法:选择确诊为膀胱尿路上皮癌的患者63例作为观察组,选择经病理证实为正常膀胱黏膜的组织63例作为对照组,应用免疫组化法检测组织中PYCR1、PCNA和Caspase-3的表达,分析PYCR1、PCNA和Caspase-3在观察组不同临床病理特征中的表达差异及与术后生存时间的相关性。结果:PYCR1和PCNA在观察组中的阳性率均明显高于对照组,Caspase-3在观察组中的阳性率明显低于对照组(均P<0.05); 观察组中PYCR1、PCNA和Caspase-3的表达与病变分级、浸润深度、脉管癌栓和是否伴鳞状分化密切相关(均P<0.05),而与性别、年龄、神经累犯无明显相关性(均P>0.05); PYCR1、PCNA和Caspase-3的表达均与术后生存时间有关; 观察组中PYCR1与PCNA具有正相关性,PYCR1与Caspase-3具有负相关性,而PCNA与Caspase-3无明显相关性。结论:膀胱尿路上皮癌中PYCR1和PCNA高表达、Caspase-3低表达均参与病变的形成和进展,联合检测PYCR1、PCNA和Caspase-3的表达对判断预后有一定价值。PYCR1与增殖指标PCNA、凋亡指标Caspase-3均有一定关联性。
Abstract:
Objective:To observe the expression characteristics of pyrrolin-5-carboxylate reductase 1(PYCR1),proliferating cell nuclear antigen(PCNA)and cysteine aspartate-specific protease-3(Caspase-3)in uroepithelial carcinoma of bladder.Methods:Sixty-three patients diagnosed with uroepithelial carcinoma of bladder were selected as the observation group,and 63 patients with normal bladder mucosa were selected as the control group.The expressions of PYCR1,PCNA and Caspase-3 were detected by immunohistochemistry.The expression differences of PYCR1,PCNA and Caspase-3 in different clinicopathological features of the observation group and their correlation with postoperative survival time were analyzed.Results:The positive rates of both PYCR1 and PCNA in the observation group were significantly higher than those in the control group,and the positive rate of Caspase-3 was significantly lower than that in the control group(all P<0.05).In the observation group,the expressions of PYCR1,PCNA and Caspase-3 were strongly correlated with lesion grade,depth of infiltration,vascular carcinoma thrombus and whether it was associated with squamous differentiation(all P<0.05),while there was no significant correlation with gender,age and nerve involvement(all P>0.05); the expressions of PYCR1,PCNA and Caspase-3 were associated with prognosis(all P<0.05); positive correlation was found between PYCR1 and PCNA,and negative correlation was found between PYCR1 and Caspase-3 in the observation group.Conclusion:High expressions of PYCR1 and PCNA,low expression of Caspase-3 are involved in the formation and progression of lesions in bladder uroepithelial carcinoma.The combined detection of PYCR1,PCNA and Caspase-3 expression is valuable for prognosis.There is a correlation between PYCR1 and PCNA,Caspase-3.

参考文献/References:

[1] 王 磊,白玉勤.膀胱尿路上皮癌组织中ERG蛋白表达水平与微血管密度和肿瘤分期相关性研究[J].陕西医学杂志,2022,51(2):245-248.
[2] Kay EJ,Paterson K,Riera-Domingo C,et al.Cancer-associated fibroblasts require proline synthesis by PYCR1 for the deposition of pro-tumorigenic extracellular matrix[J].Nat Metab,2022,4(6):693-710.
[3] Oudaert I,Satilmis H,Vlummens P,et al.Pyrroline-5-carboxylate reductase 1:A novel target for sensitizing multiple myeloma cells to bortezomib by inhibition of PRAS40-mediated protein synthesis[J].J Exp Clin Cancer Res,2022,41(1):45-46.
[4] Wang J,Zhang X,Wei P,et al.Livin,Survivin and Caspase 3 as early recurrence markers in non-muscle-invasive bladder cancer[J].World J Urol,2014,32(6):1477-1478.
[5] 刘爱东,张 爽,王丹琳,等.MMP-19在胃腺癌中的表达及与神经型钙黏素的相关性[J].重庆医科大学学报,2022,47(2):145-150.
[6] 张 琳,高 勇.复方红豆杉胶囊对Walker-256移植性肝癌大鼠HIF-1α、VEGF及PCNA表达的影响[J].陕西中医,2019,40(2):148-151,155.
[7] 徐 刚,肖尚文,梅述怀,等.补中益气与大柴胡汤直肠灌滴治疗膀胱肿瘤术后及对胃肠功能的影响[J].陕西中医,2013,34(9):1184-1186.
[8] 梁应凤,郭姗姗,张 丹,等.健脾益肾方对非小细胞肺癌细胞体外增殖凋亡作用的实验研究[J].陕西中医,2020,41(5):574-577.
[9] Shenoy A,Belugali N,Perry G,et al.Proteomic patterns associated with response to breast cancer neoadjuvant treatment[J].Mol Syst Biol,2020,16(9):9443.
[10] 程秋华,周 帅,向 杨,等.PYCR1基因在肝细胞癌中的表达及与预后的关系[J].实用医学杂志,2021,37(2):233-237.
[11] Xu Y,Zuo W,Wang X,et al.Deciphering the effects of PYCR1 on cell function and its associated mechanism in hepatocellular carcinoma[J].Int J Biol Sci,2021,17(9):2223-2239.
[12] 秦 曦,姜 政.PYCR1在结肠癌中的表达及对细胞的增殖、侵袭及迁移的影响[J].第三军医大学学报,2019,41(20):1961-1968.
[13] 孙 娟,林 晖,王耀新,等.PYCR1对胃癌细胞增殖和侵袭的影响[J].现代肿瘤医学,2021,29(12):2030-2035.
[14] Westbrook RL,Bridges E,Roberts J,et al.Proline synthesis through PYCR1 is required to support cancer cell proliferation and survival in oxygen-limiting conditions[J].Cell Rep,2022,38(5):110320.
[15] Zhuang J,Song Y,Ye Y,et al.PYCR1 interference inhibits cell growth and survival via c-Jun N-terminal kinase/insulin receptor substrate 1(JNK/IRS1)pathway in hepatocellular cancer[J].J Transl Med,2019,17(1):343.
[16] Wang D,Wang L,Zhang Y,et al.PYCR1 promotes the progression of non-small-cell lung cancer under the negative regulation of miR-488[J].Biomed Pharmacother,2019,111:588-595.
[17] Xiao S,Yao X,Ye J,et al.Epigenetic modification facilitates proline synthase PYCR1 aberrant expression in gastric cancer[J].Biochim Biophys Acta Gene Regul Mech,2022,1865(6):194829.
[18] Cheng C,Song D,Wu Y,et al.RAC3 promotes proliferation,migration and invasion via PYCR1/JAK/STAT signaling in bladder cancer[J].Front Mol Biosci,2020,7:218.
[19] Kuo ML,Lee MB,Tang M,et al.PYCR1 and PYCR2 interact and collaborate with RRM2B to protect cells from overt oxidative stress[J].Sci Rep,2016,6:18846.
[20] Zhang T,Liu Y,Liu W,et al.Increased PYCR1 mRNA predicts poor prognosis in kidney adenocarcinoma:A study based on TCGA database[J].Medicine(Baltimore),2021,100(38):27145.
[21] 刘爱东,刘时飞,熊光宜,等.真核翻译起始因子2α促进结直肠腺癌细胞增殖[J].中华实验外科杂志,2022,39(9):1752-1754.

相似文献/References:

[1]王 磊,白玉勤.膀胱尿路上皮癌组织中ERG蛋白表达水平与微血管密度和肿瘤分期相关性研究[J].陕西医学杂志,2022,51(2):245.[doi:DOI:10.3969/j.issn.1000-7377.2022.02.027]
　WANG Lei,BAI Yuqin.Correlation between ERG protein expression level and microvessel density,tumor stage in bladder transition cell carcinoma[J].,2022,51(8):245.[doi:DOI:10.3969/j.issn.1000-7377.2022.02.027]

备注/Memo

备注/Memo:
基金项目:中国医疗手牵手工程委员会-北京医学奖励基金会项目(YXJL-2021-1092-0640)
更新日期/Last Update: 2023-08-07