[1]李威威,杨 倩,常 瑚,等.妊娠期糖尿病视网膜病变患者血清miR-200c-3p、miR-20b-5p表达水平及检测临床意义[J].陕西医学杂志,2023,52(8):1076-1080.[doi:DOI:10.3969/j.issn.1000-7377.2023.08.031]
　LI Weiwei,YANG Qian,CHANG Hu,et al.Expression levels and clinical significance of serum miR-200c-3p and miR-20b-5p in patients with gestational diabetic retinopathy[J].,2023,52(8):1076-1080.[doi:DOI:10.3969/j.issn.1000-7377.2023.08.031]
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妊娠期糖尿病视网膜病变患者血清miR-200c-3p、miR-20b-5p表达水平及检测临床意义
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年8期
页码:
1076-1080
栏目:
临床检验
出版日期:
2023-08-05

文章信息/Info

Title:
Expression levels and clinical significance of serum miR-200c-3p and miR-20b-5p in patients with gestational diabetic retinopathy
作者:
李威威1; 杨 倩1; 常 瑚1; 宋 森1; 李世强1; 程亚辉1; 岳 钟1; 冯 丽1; 肖博文2
(1.保定市第一中心医院眼科,河北 保定071000; 2.天津市北辰医院眼科,天津 300134)
Author(s):
LI Weiwei; YANG Qian; CHANG Hu; SONG Sen; LI Shiqiang; CHENG Yahui; YUE Zhong; FENG Li; XIAO Bowen
(Department of Ophthalmology,Baoding No.1 Central Hospital,Baoding 071000,China)
关键词:
妊娠期糖尿病视网膜病变;  miR-200c-3p;  miR-20b-5p;  表达水平;  预后;  诊断
Keywords:
Gestational diabetic retinopathy;  miR-200c-3p;  miR-20b-5p;  Expression level;  Prognosis;  Diagnosis
分类号:
R 587.1
DOI:
DOI:10.3969/j.issn.1000-7377.2023.08.031
文献标志码:
A
摘要:
目的:探讨妊娠期糖尿病视网膜病变(DR)患者血清miR-200c-3p、miR-20b-5p水平变化及检测临床意义。方法:选择妊娠期糖尿病孕妇80例(GDM组)和DR孕妇75例(DR组),正常妊娠期女性95例(对照组)。采用qRT-PCR法对受试者血清miR-200c-3p、miR-20b-5p水平进行测定,采用全自动生化分析仪对受试者低密度脂胆固醇(LDL-C)、三酰甘油(TG)、总胆固醇(TC)进行测定,采用血糖检测仪对受试者空腹血糖(FPG)、餐后2 h血糖(2 hPG)进行测定。结果:GDM组、DR组患者FPG、2 hPG、LDL-C、TG、TC、miR-200c-3p、miR-20b-5p值显著高于对照组(均P<0.05); DR组患者FPG、2 hPG、LDL-C、TG、TC、miR-200c-3p、miR-20b-5p值显著高于GDM组(均P<0.05); 随着病情严重程度的增加,DR组患者miR-200c-3p、miR-20b-5p水平显著升高(均P<0.05); 预后不良组DR患者FPG、2 hPG、LDL-C、TG、TC、miR-200c-3p、miR-20b-5p值显著高于预后良好组(均P<0.05); 多因素Logistic回归分析显示血清miR-200c-3p、miR-20b-5p水平升高为DR患者发生预后不良的危险因素; ROC曲线结果显示,血清miR-200c-3p、miR-20b-5p、两者联合诊断DR患者不良预后曲线下面积(AUC)为0.927(95%CI:0.843～0.974)、0.848(95%CI:0.746～0.920)、0.985(95%CI:0.924～0.999)。结论:DR患者血清miR-200c-3p、miR-20b-5p水平升高,对DR不良预后具有一定的预测价值。
Abstract:
Objective:To explore the levels and clinical significance of serum miR-200c-3p and miR-20b-5p in patients with gestational diabetic retinopathy(DR).Methods:A total of 80 pregnant women with gestational diabetes mellitus(GDM group),75 pregnant women with DR(DR group)and 95 women with normal pregnancy(control group)were selected.The serum levels of miR-200c-3p and miR-20b-5p were measured by qRT-PCR.Low-density lipid cholesterol(LDL-C),triglyceride(TG)and total cholesterol(TC)were measured by automatic biochemical analyzer.Fasting plasma glucose(FPG)and 2-hour postprandial plasma glucose(2 hPG)were measured by blood glucose detector.Results:Compared with the control group,the FPG,2 hPG,LDL-C,TG,TC,serum miR-200c-3p and miR-20b-5p values in the GDM and DR groups were significantly increased(all P<0.05).Compared with the GDM group,the FPG,2 hPG,LDL-C,TG,TC,serum miR-200c-3p and miR-20b-5p values in the DR group were significantly increased(all P<0.05).As the severity of the disease increased,the levels of miR-200c-3p and miR-20b-5p in DR patients increased significantly(all P<0.05).Compared with the good prognosis group,the FPG,2 hPG,LDL-C,TG,TC,serum miR-200c-3p and miR-20b-5p values of DR patients in the poor prognosis group were significantly increased(all P<0.05).Multivariate Logistic regression analysis showed that elevated serum miR-200c-3p and miR-20b-5p levels were risk factors for poor prognosis in patients with DR.ROC curve results showed that the AUC of serum miR-200c-3p,miR-20b-5p,and the combined diagnosis for poor prognosis in patients with DR was 0.927(95%CI:0.843 to 0.974),0.848(95%CI:0.746 to 0.920),0.985(95%CI:0.924 to 0.999)respectively.Conclusion:The serum miR-200c-3p and miR-20b-5p levels of DR patients are elevated,which has certain diagnostic value for the poor prognosis of DR.

参考文献/References:

[1] 李亚妮,肖景华,计 静,等.妊娠期糖尿病患者孕晚期阴道微生态状况、血清网膜素、促代谢因子水平变化及临床意义[J].陕西医学杂志,2022,51(11):1372-1375,1380.
[2] Sweeting A,Wong J,Murphy HR,et al.A clinical update on gestational diabetes mellitus[J].Endocr Rev,2022,43(5):763-793.
[3] Ridderskar L,Montan P,Kugelberg M,et al.Outcome of cataract surgery in eyes with diabetic retinopathy:A Swedish national cataract register report[J].Acta Ophthalmol,2022,100(2):571-577.
[4] Li W,Yang S,Chen G,et al.miR-200c-3p regulates pyroptosis by targeting SLC30A7 in diabetic retinopathy[J].Hum Exp Toxicol,2022,41:1-9.
[5] Vashukova ES,Kozyulina PY,Illarionov RA,et al.High-throughput sequencing of circulating micrornas in plasma and serum during pregnancy progression[J].Life(Basel),2021,11(10):1055.
[6] Ma Y,Dong C,Chen X,et al.Silencing of miR-20b-5p exerts inhibitory effect on diabetic retinopathy via inactivation of thbs1 gene induced VEGF/Akt/PI3K pathway[J].Diabetes Metab Syndr Obes,2021,14:1183-1193.
[7] 杨慧霞,徐先明,王子莲,等.妊娠合并糖尿病诊治指南(2014)[J].中华围产医学杂志,2014,17(8):537-545.
[8] 汪 佶,张贵华,林建伟,等.深度学习中标准化人工标注在早产儿视网膜病变图像识别中的应用[J].中华实验眼科杂志,2019,37(8):653-657.
[9] 汤琳琳,王雅萍.妊娠期糖尿病妇女血浆IL-1Ra水平及其与产后2型糖尿病的相关性研究[J].现代医学,2022,50(7):864-870.
[10] Hehua Z,Yang X,Qing C,et al.Dietary patterns and associations between air pollution and gestational diabetes mellitus[J].Environ Int,2021,147(1):106347.
[11] Benfica CZ,Zanella T,Farias LB,et al.Macular choroidal thickness in pregnant women with type 1,type 2 and gestational diabetes mellitus measured by spectral-domain optical coherence tomography[J].Clin Ophthalmol,2018,12(1):1259-1265.
[12] Feldman-Billard S,Dupas B.Eye disorders other than diabetic retinopathy in patients with diabetes[J].Diabetes Metab,2021,47(6):101279.
[13] 刘秋杨,朱慧芳,王迎春,等.黄芪四君子汤联合门冬胰岛素治疗妊娠期糖尿病临床研究[J].陕西中医,2021,42(10):1408-1411.
[14] Borilova LP,Kavrikova D,Tomandlova M,et al.Differences in interleukin-8 plasma levels between diabetic patients and healthy individuals independently on their periodontal status[J].Int J Mol Sci,2018,19(10):3214.
[15] Du Y,Chi X,An W.Downregulation of microRNA-200c-3p reduces damage of hippocampal neurons in epileptic rats by upregulating expression of RECK and inactivating the AKT signaling pathway[J].Chem Biol Interact,2019,307:223-233.
[16] 杨晓岗,储昭节,张 婷.核苷酸结合寡聚化结构域样受体蛋白3炎症小体对糖尿病性视网膜病变新生血管形成和血管拟态标志物的影响[J].陕西医学杂志,2021,50(11):1412-1415.
[17] 高志强,马 云,崔玉彬.糖尿病肾病患者血清miR-200c与TGF-β1、FN的相关性分析[J].山东医药,2018,58(14):50-52.
[18] Chen K,Yu T,Wang X.Inhibition of circulating exosomal miRNA-20b-5p accelerates diabetic wound repair[J].Int J Nanomedicine,2021,16:371-381.
[19] 冀 杰,罗艳华,孔 宁,等.消朦灵方在糖尿病视网膜病变大鼠中的应用价值[J].陕西中医,2021,42(10):1344-1348.
[20] Zhu K,Hu X,Chen H,et al.Downregulation of circRNA DMNT3B contributes to diabetic retinal vascular dysfunction through targeting miR-20b-5p and BAMBI[J].EBio Medicine,2019,49:341-353.

备注/Memo

备注/Memo:
基金项目:天津市科技计划项目(SHGY-2020026); 河北省保定市科技计划项目(2041ZF093)
更新日期/Last Update: 2023-08-07