[1]吴俊林,许 莹,张发荣.不同浓度尿酸盐对人肝THLE-2细胞钙代谢的影响及机制研究[J].陕西医学杂志,2023,52(8):965-968.[doi:DOI:10.3969/j.issn.1000-7377.2023.08.005]
 WU Junlin,XU Ying,ZHANG Farong.Effects of different concentrations of urate on calcium metabolism in human liver THLE-2 cells[J].,2023,52(8):965-968.[doi:DOI:10.3969/j.issn.1000-7377.2023.08.005]
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不同浓度尿酸盐对人肝THLE-2细胞钙代谢的影响及机制研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年8期
页码:
965-968
栏目:
基础研究
出版日期:
2023-08-05

文章信息/Info

Title:
Effects of different concentrations of urate on calcium metabolism in human liver THLE-2 cells
作者:
吴俊林1许 莹1张发荣2
(1.武汉市第五医院内分泌科,湖北 武汉 430050; 2.武汉市东西湖第二人民医院耳鼻喉科,湖北 武汉 430040)
Author(s):
WU JunlinXU YingZHANG Farong
(Department of Endocrinology,Wuhan Fifth Hospital,Wuhan 430050,China)
关键词:
尿酸 非酒精性脂肪肝 肝细胞 钙代谢 三磷酸肌醇受体
Keywords:
Uric acid Nonalcoholic fatty liver Hepatocyte Calcium metabolism Inositol triphosphate receptor
分类号:
R 589.7
DOI:
DOI:10.3969/j.issn.1000-7377.2023.08.005
文献标志码:
A
摘要:
目的:观察不同浓度尿酸盐对人肝THLE-2细胞钙代谢的影响及机制。方法:体外培养人肝THLE-2细胞,取生长状态佳且处于对数生长期的THLE-2细胞,将其分为A组(阴性对照组)、B组(360 mol/L尿酸盐)、C组(600 mol/L尿酸盐)三个实验组; 各组干预THLE-2细胞48 h后,采用Fluo-3/AM荧光探针测定细胞内Ca2+浓度,实时定量RT-PCR及Western blot法检测细胞三磷酸肌醇受体(IP3R)mRNA及蛋白表达。结果:B组与C组中THLE-2细胞内Ca2+浓度均高于A组(均P<0.05),B组与C组中IP3R mRNA的表达水平均高于A组(均P<0.05),B组与C组中THLE-2细胞中IP3R蛋白表达水平均高于A组(均P<0.05); B组IP3R mRNA和蛋白表达水平均低于C组(均P<0.05)。结论:高尿酸血症可明显影响人肝THLE-2细胞钙离子水平,其机制可能与升高THLE-2细胞IP3R mRNA及蛋白表达水平、影响内质网及线粒体钙稳态有关。
Abstract:
Objective:To observe the effect of different concentrations of urate on calcium metabolism of hepatocytes and its mechanism.Methods:Liver THLE-2 cells were cultured in vitro,and THLE-2 cells with good growth condition and logarithmic growth stage were selected and divided into group A(negative control group),group B(360 mol/L urate)and group C(600 mol/L urate).After 48 hours intervention in THLE-2 cells,the intracellular Ca2+ concentration was determined by Fluo-3/AM fluorescence probe,and the mRNA and protein expression of inositol triphosphate receptor(IP3R)were detected by real-time quantitative RT-PCR and Western blot.Results:The concentration of Ca2+ in THLE-2 cells in group B and C was higher than that in the control group(all P<0.05).The expression level of IP3R mRNA in group B and C was higher than that in group A(all P<0.05).IP3R protein concentration in THLE-2 cells in group B and C was higher than that in group A(all P<0.05).The expression levels of IP3R mRNA and protein in group B were lower than those in group C(all P<0.05)Conclusion:Hyperuricemia can significantly affect the calcium ion level in liver THLE-2 cells,and the mechanism may be related to the increase of IP3R mRNA and protein expression levels in liver THLE-2 cells,and the influence of calcium homeostasis in endoplasmic reticulum and mitochondria.

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备注/Memo

备注/Memo:
基金项目:武汉市卫生健康委员会科研项目(WX20Z21)
更新日期/Last Update: 2023-08-07