[1]赵莉娅,赵铁铮,庞 英,等.乐伐替尼对甲状腺癌SW579细胞放射敏感性及血管内皮生长因子、碱性成纤维细胞生长因子、碱性成纤维细胞生长因子受体表达的影响[J].陕西医学杂志,2023,52(8):956-959,968.[doi:DOI:10.3969/j.issn.1000-7377.2023.08.003]
　ZHAO Liya,ZHAO Tiezheng,PANG Ying,et al.Effects of lenvatinib on radiosensitivity and expression of VEGF,bFGF and bFGFR in thyroid cancer SW579 cells[J].,2023,52(8):956-959,968.[doi:DOI:10.3969/j.issn.1000-7377.2023.08.003]
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乐伐替尼对甲状腺癌SW579细胞放射敏感性及血管内皮生长因子、碱性成纤维细胞生长因子、碱性成纤维细胞生长因子受体表达的影响
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年8期
页码:
956-959,968
栏目:
基础研究
出版日期:
2023-08-05

文章信息/Info

Title:
Effects of lenvatinib on radiosensitivity and expression of VEGF,bFGF and bFGFR in thyroid cancer SW579 cells
作者:
赵莉娅1; 赵铁铮2; 庞 英2; 杨 娜2; 范彦军1
(1.石家庄市急救中心特勤大队,河北 石家庄 050000; 2.黄骅市人民医院普外科,河北 黄骅 061100)
Author(s):
ZHAO Liya; ZHAO Tiezheng; PANG Ying; YANG Na; FAN Yanjun
(Special Service Brigade of Shijiazhuang Emergency Center,Shijiazhuang 050000,China)
关键词:
乐伐替尼;  甲状腺癌SW579细胞;  血管内皮生长因子;  碱性成纤维细胞生长因子;  碱性成纤维细胞生长因子受体;  放射敏感性
Keywords:
Levatinib;  Thyroid cancer SW579 cells;  Vascular endothelial growth factor;  Basic fibroblast growth factor;  Basic fibroblast growth factor receptor;  Radiosensitivity
分类号:
R 736.1
DOI:
DOI:10.3969/j.issn.1000-7377.2023.08.003
文献标志码:
A
摘要:
目的:探究乐伐替尼对甲状腺癌SW579细胞放射敏感性以及对血管内皮生长因子(VEGF)、碱性成纤维细胞生长因子(bFGF)及其受体(bFGFR)表达的影响。方法:体外培养甲状腺癌SW579细胞,筛选最佳放射剂量,取培养至对数生长期的SW579细胞分为对照组(常规培养SW579细胞)、放射组(使用4 Gy X射线照射SW579细胞)、乐伐替尼低(在含SW579细胞的培养基中加入5 μmol/L的乐伐替尼后,再使用4 Gy X射线照射SW579细胞)、高(在含SW579细胞的培养基中加入10 μmol/L的乐伐替尼后,再使用4 Gy X射线照射SW579细胞)浓度组,培养48 h后。检测SW579细胞存活率、细胞凋亡率和细胞中B细胞淋巴瘤-2相关X(Bax)、B细胞淋巴瘤-2(Bcl-2)、活化胱天蛋白酶3(Cleaved caspase-3)、VEGF、bFGF、bFGFR蛋白表达。结果:随着放射剂量的升高,SW579细胞存活率依次降低(均P<0.05),选取4 Gy X射线进行后续实验; 与对照组相比,放射组、乐伐替尼低、高浓度组SW579细胞存活率、Bcl-2、VEGF、bFGF、bFGFR蛋白表达依次降低(均P<0.05),细胞凋亡率、Bax、Cleaved caspase-3蛋白表达依次升高(均P<0.05)。结论:乐伐替尼能增强SW579细胞的放射敏感性,其机制可能与抑制VEGF、bFGF、bFGFR蛋白表达有关。
Abstract:
Objective:To investigate the effects of lenvatinib on the radiosensitivity and the expression of vascular endothelial growth factor(VEGF),basic fibroblast growth factor(bFGF)and its receptor(bFGFR)in thyroid cancer SW579 cells.Methods:Thyroid cancer SW579 cells were cultured in vitro to screen the optimal dose of radiation.SW579 cells cultured to logarithmic growth stage were divided into control group(conventional culture SW579 cells),radiation group(SW579 cells were irradiated with 4 Gy X-ray),levastinib low concentration group(after adding 5 μmol/L of levatinib to the medium containing SW579 cells,SW579 cells were irradiated with 4 Gy X-ray)and levastinib high concentration group(after adding 10 μmol/L of levatinib to the medium containing SW579 cells,SW579 cells were irradiated with 4 Gy X-ray),and cultured for 48 hours.Cell survival rate,apoptosis rate,the protein expressions of B-cell lymphoma-2-associated X protein(Bax),B-cell lymphoma-2(Bcl-2),activated Caspase-3(Cleaved caspase-3),VEGF,bFGF,and bFGFR in SW579 were detected.Results:With the increase of radiation dose,the survival rate of SW579 cells decreased in turn(all P<0.05),and 4 Gy X-ray was selected for follow-up experiments.Compared with control group,survival rate,protein expressions of Bcl-2,VEGF,bFGF and bFGFR of SW579 cells in radiation group,lovastinib low concentration group and high concentration group were decreased in turn,and the apoptosis rate,protein expressions of Bax,Cleaved caspase-3 were increased in turn(all P<0.05).Conclusion:Lenvatinib can enhance the radiosensitivity of SW579 cells,and the mechanism may be related to the inhibition of VEGF,bFGF and bFGFR protein expressions.

参考文献/References:

[1] 石维超,张少波.甲状腺癌病理微血管密度与颈部淋巴结转移相关性研究[J].陕西医学杂志,2022,51(11):1449-1452.
[2] Kwong DLW,Chan WWL.External radiotherapy for locoregional control in thyroid carcinoma[J].Methods Mol Biol,2022,2534(1):215-224.
[3] Jacomina LE,Jacinto JKM,Co LBA,et al.The Role of postoperative external beam radiotherapy for differentiated thyroid carcinoma:A systematic review and meta-analysis[J].Head Neck,2020,42(8):2181-2193.
[4] Dierks C,Seufert J,Aumann K,et al.Combination of lenvatinib and pembrolizumab is an effective treatment option for anaplastic and poorly differentiated thyroid carcinoma[J].Thyroid,2021,31(7):1076-1085.
[5] Boudin L,Morvan JB,Thariat J,et al.Rationale efficacy and safety evidence of lenvatinib and pembrolizumab association in anaplastic thyroid carcinoma[J].Curr Oncol,2022,29(10):7718-7731.
[6] Ionescu C,Oprea B,Ciobanu G,et al.The angiogenic balance and its implications in cancer and cardiovascular diseases:An overview[J].Medicina(Kaunas),2022,58(7):903.
[7] 郭忠聪,龚 纯.抗癌防移片对大肠癌肝转移小鼠血清肝细胞生长因子、血管内皮生长因子表达的影响[J].陕西中医,2022,43(11):1527-1530,1604.
[8] Zahra FT,Sajib MS,Mikelis CM.Role of bFGF in acquired resistance upon anti-VEGF therapy in cancer[J].Cancers(Basel),2021,13(6):1422.
[9] Deng H,Kan A,Lyu N,et al.Dual vascular endothelial growth factor receptor and fibroblast growth factor receptor inhibition elicits antitumor immunity and enhances programmed cell death-1 checkpoint blockade in hepatocellular carcinoma[J].Liver Cancer,2020,9(3):338-357.
[10] 董博文,李子一,李伟东,等.双硫仑联合铜离子对乐伐替尼耐药肝癌细胞Huh7增殖及凋亡的影响[J].中国普外基础与临床杂志,2020,27(2):168-172.
[11] Nabhan F,Dedhia PH,Ringel MD.Thyroid cancer,recent advances in diagnosis and therapy[J].Int J Cancer,2021,149(5):984-992.
[12] 傅 强,黄学阳,王建春,等.养阴散结汤辅助治疗分化型甲状腺癌疗效及对患者术后甲状腺、免疫功能和血清学指标的影响[J].陕西中医,2022,43(8):1029-1031,1036.
[13] 丛仔红,张元会.超声引导喉上神经阻滞联合颈神经通路阻滞在甲状腺癌根治术中的应用研究[J].陕西医学杂志,2022,51(11):1408-1411.
[14] Privitera F,Gioco R,Fazio I,et al.Risk factors for low levels of parathyroid hormone after surgery for thyroid cancer:A single center study[J].J Clin Med,2021,10(18):4113.
[15] Blomain E,Berta S,Hug N,et al.Radiotherapy for brain metastases from thyroid cancer:An institutional and national retrospective cohort study[J].Thyroid,2022,32(7):781-788.
[16] Nair A,Lemery SJ,Yang J,et al.FDA approval summary:Lenvatinib for progressive,radio-iodine-refractory differentiated thyroid cancer[J].Clin Cancer Res,2015,21(23):5205-5208.
[17] Loftus LV,Amend SR,Pienta KJ.Interplay between cell death and cell proliferation reveals new strategies for cancer therapy[J].Int J Mol Sci,2022,23(9):4723.
[18] Catalano M,Casadei-Gardini A,Vannini G,et al.Lenvatinib:Established and promising drug for the treatment of advanced hepatocellular carcinoma[J].Expert Rev Clin Pharmacol,2021,14(11):1353-1365.
[19] Li Y,Wang Y,Yu X,et al.Radix tetrastigma inhibits the non-small cell lung cancer via Bax/Bcl-2/Caspase-9/Caspase-3 pathway[J].Nutr Cancer,2022,74(1):320-332.
[20] Xia X,Wang S,Wu L,et al.TIPE2 attenuates neuroinflammation and brain injury through Bcl-2/Bax/Cleaved caspase-3 apoptotic pathways after intracerebral hemorrhage in mice[J].Brain Res Bull,2022,191(1):1-8.
[21] Lugano R,Ramachandran M,Dimberg A.Tumor angiogenesis:Causes,consequences,challenges and opportunities[J].Cell Mol Life Sci,2020,77(9):1745-1770.
[22] Olejarz W,Kubiak-Tomaszewska G,Chrzanowska A,et al.Exosomes in angiogenesis and anti-angiogenic therapy in cancers[J].Int J Mol Sci,2020,21(16):5840.
[23] Park M,Jung HL,Shim YJ,et al.Serum cytokine profiles in infants with infantile hemangiomas on oral propranolol treatment:VEGF and bFGF,potential biomarkers predicting clinical outcomes[J].Pediatr Res,2020,88(5):749-755.
[24] Wang S,Liu Z,Ma YM,et al.Upregulated insulin receptor tyrosine kinase substrate promotes the proliferation of colorectal cancer cells via the bFGF/AKT signaling pathway[J].Gastroenterol Rep(Oxf),2020,9(2):166-175.
[25] Bariya D,Mishra SP,Akshay BR,et al.Relationship between vascular endothelial growth factor expression and thyroid stimulating hormone level in benign and malignant thyroid lesions[J].J Family Med Prim Care,2022,11(6):2565-2572.
[26] Jia ZY,Wu XL,Zhang YH,et al.The correlation between ultrasonographic features,bFGF,and the local invasiveness of thyroid papillary carcinoma[J].Medicine(Baltimore),2020,99(26):20644.

备注/Memo

备注/Memo:
基金项目:河北省医学科学研究计划项目(20210533)
更新日期/Last Update: 2023-08-07