[1]张文涛,邹 斌,蔡粤川,等.白头翁皂苷A调控circ-0001742对鼻咽癌C666-1细胞恶性生物学行为影响实验研究[J].陕西医学杂志,2023,52(8):952-955,964.[doi:DOI:10.3969/j.issn.1000-7377.2023.08.002]
　ZHANG Wentao,ZOU Bin,CAI Yuechuan,et al.Effects of pulsatilla saponin A regulating circ-0001742 on malignant biological behavior of nasopharyngeal carcinoma C666-1 cells[J].,2023,52(8):952-955,964.[doi:DOI:10.3969/j.issn.1000-7377.2023.08.002]
点击复制

白头翁皂苷A调控circ-0001742对鼻咽癌C666-1细胞恶性生物学行为影响实验研究
分享到：

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年8期
页码:
952-955,964
栏目:
基础研究
出版日期:
2023-08-05

文章信息/Info

Title:
Effects of pulsatilla saponin A regulating circ-0001742 on malignant biological behavior of nasopharyngeal carcinoma C666-1 cells
作者:
张文涛; 邹 斌; 蔡粤川; 汪 俊
(乐山市中医医院耳鼻咽喉科,四川 乐山614000)
Author(s):
ZHANG Wentao; ZOU Bin; CAI Yuechuan; WANG Jun
(Department of Otolaryngology,Leshan Hospital of Traditional Chinese Medicine,Leshan 614000,China)
关键词:
白头翁皂苷A;  circ-0001742;  鼻咽癌;  细胞增殖;  细胞凋亡;  细胞迁移
Keywords:
Pulsatilla saponin A;  circ-0001742;  Nasopharyngeal carcinoma;  Cell proliferation;  Apoptosis;  Cell migration
分类号:
R 739.6
DOI:
DOI:10.3969/j.issn.1000-7377.2023.08.002
文献标志码:
A
摘要:
目的:探讨白头翁皂苷A对鼻咽癌细胞恶性生物学行为的影响及分子机制。方法:体外培养人鼻咽上皮细胞NP69和鼻咽癌细胞系(HK-1、HONE1、CNE2、SUNE1、C666-1),实时荧光定量PCR(RT-qPCR)检测细胞中circ-0001742表达水平; 将C666-1细胞分为si-circ-0001742组、si-NC组、对照组、白头翁皂苷A-L、M、H组、白头翁皂苷A-H+pcDNA-circ-0001742组; 四甲基偶氮唑盐比色法(MTT)检测细胞存活率,流式细胞术检测细胞凋亡,Transwell检测细胞迁移数,克隆形成实验检测细胞克隆数,蛋白质印迹法检测蛋白表达。结果:鼻咽癌细胞(HK-1、HONE1、CNE2、SUNE1、C666-1)中circ-0001742表达水平升高(均P<0.05)。沉默circ-0001742后,C666-1细胞增殖抑制率、细胞凋亡率以及活化的半胱天冬氨酸蛋白酶3(Cleaved caspase-3)表达水平升高,C666-1细胞迁移和克隆细胞数减少(均P<0.05)。不同浓度白头翁皂苷A处理后,C666-1细胞增殖抑制率、细胞凋亡率以及Cleaved caspase-3表达水平升高,C666-1细胞迁移和克隆细胞数减少,circ-0001742表达水平降低,呈浓度依赖性(均P<0.05)。circ-0001742过表达可逆转白头翁皂苷A对C666-1细胞的抑制作用。结论:白头翁皂苷A通过下调circ-0001742抑制鼻咽癌C666-1细胞恶性生物学行为。
Abstract:
Objective:To explore the effects of pulsatilla saponin A on the malignant biological behavior of nasopharyngeal carcinoma cells and its molecular mechanism.Methods:Human nasopharyngeal epithelial cells NP69 and nasopharyngeal carcinoma cell lines(HK-1,HONE1,CNE2,SUNE1,C666-1)were cultured in vitro,and the expression level of circ-0001742 was detected by RT-qPCR.C666-1 cells were divided into si-circ-0001742 group,si-NC group,control group,Pulsatilla saponin A-L,M,H group,Pulsatilla saponin A-H+pcDNA-circ-0001742 group.The cell survival rate was determined by MTT.Apoptosis was detected by flow cytometry.Transwell was used to detect the number of cell migration.The clone formation test was used to detect the number of cell clones.Western blot was used to detect protein expression.Results:The expression levels of circ-0001742 in nasopharyngeal carcinoma cells(HK-1,HONE1,CNE2,SUNE1,C666-1)increased(all P<0.05).After circ-0001742 silenced,C666-1 cell proliferation inhibition rate,cell apoptosis rate,Cleaved caspase-3 expression level increased,C666-1 cell migration and number of cloned cells decreased(all P<0.05).After treatment with different concentrations,C666-1 cell proliferation inhibition rate,cell apoptosis rate,Cleaved caspase-3 expression level increased,C666-1 cell migration and number of cloned cells decreased,and circ-0001742 expression level decreased in a concentration-dependent manner(all P<0.05).Overexpression of circ-0001742 reversed the inhibitory effect of pulsatilla saponin A on C666-1 cells.Conclusion:Pulsatilla saponin A inhibits the malignant biological behavior of nasopharyngeal carcinoma C666-1 cells by down-regulating circ-0001742.

参考文献/References:

[1] Jiang X,Deng X,Wang J,et al.BPIFB1 inhibits vasculogenic mimicry via downregulation of GLUT1-mediated H3K27 acetylation in nasopharyngeal carcinoma[J].Oncogene,2022,41(2):233-245.
[2] Huang J,Fang J,Xu X,et al.SETD1A promotes the proliferation and glycolysis of nasopharyngeal carcinoma cells by activating the PI3K/Akt pathway[J].Open Med(Wars),2022,17(1):1849-1859.
[3] Li W,Xu R,Zhu B,et al.circular RNAs:Functions and mechanisms in nasopharyngeal carcinoma[J].Head Neck,2022,44(2):494-504.
[4] 肖白玉,党 华,甘嘉裕,等.实时荧光定量PCR检测鼻咽癌组织中PTCH-1和SMO基因的表达[J].细胞与分子免疫学杂志,2010,26(10):955-958.
[5] Shao B,He L.Has-circ-0001742 promotes tongue squamous cell carcinoma progression via modulating miR-634 expression[J].Biochem Biophys Res Commun,2019,513(1):135-140.
[6] 喻 凤,王林林.中医药治疗鼻咽癌研究进展[J].中医眼耳鼻喉杂志,2019,9(2):99-101.
[7] Song YC,Hung KF,Liang KL,et al.Adjunctive Chinese herbal medicine therapy for nasopharyngeal carcinoma:Clinical evidence and experimental validation[J].Head Neck,2019,41(9):2860-2872.
[8] 王冠玲,何 文.白头翁皂苷抗肿瘤作用的研究进展[J].中国药师,2019,22(4):152-154.
[9] 潘炜娟,丁 玲.不同浓度白头翁皂苷对口腔鳞癌细胞增殖、迁移和钙蛋白酶1、E-cadherin、N-cadherin蛋白的影响[J].中国药师,2018,21(6):40-44.
[10] Ye B,Zhou Y,Liu Y,et al.Pulsatilla saponin A induces apoptosis and differentiation of myeloma cells[J].Anticancer Agents Med Chem,2021,21(7):919-926.
[11] 彭超军,焦 旸.白头翁皂苷A对肺癌放射敏感性影响的初步研究[J].中国科技论文在线,2016,9(5):464-469.
[12] 易亭伍,梁 睿,宿向东.长链非编码RNA SNHG15通过miR-141-3p/KLF9机制调控鼻咽癌细胞增殖的实验研究[J].陕西医学杂志,2021,50(1):10-14.
[13] 苏建淳,张国义,卢秋霞,等.miR-124在鼻咽癌组织中的表达及其与预后关系研究[J].陕西医学杂志,2020,49(2):144-147.
[14] Sun Y,Liu Y,Du Z,et al.CircSOX9 acts as a molecular sponge of miR-485-3p to promote the progression of nasopharyngeal carcinoma[J].Aging(Albany NY),2022,14(11):4914-4926.
[15] Yao Y,Bi L,Zhang C.circular RNA-0001742 has potential to predict advanced tumor stage and poor survival profiles in tongue squamous cell carcinoma management[J].J Clin Lab Anal,2020,34(8):23330.
[16] Hu YT,Li XX,Zeng LW.circ-0001742 promotes tongue squamous cell carcinoma progression via miR-431-5p/ATF3 axis[J].Eur Rev Med Pharmacol Sci,2019,23(23):10300-10312.
[17] 黄金圣,黄学武.养阴清热法对鼻咽癌放疗后患者敏感性的影响[J].陕西中医,2015,36(9):1195-1197.
[18] 马国辉,董艳玲,吴 楠,等.养阴解毒汤对气阴两虚型晚期鼻咽癌放化疗患者免疫功能和癌因性疲乏的影响[J].陕西中医,2022,43(10):1407-1410.
[19] 邹晓静,郝燕燕,胡一迪,等.白头翁皂苷A通过调控miR-24-3p/RNF2的表达影响乳腺癌细胞增殖及放射敏感性[J].中国病理生理杂志,2020,36(5):865-870.
[20] Xu L,Cheng G,Lu Y,et al.An active molecule from pulsatillachinensis,pulsatill asaponin A,induces apoptosis and inhibits tumor growth of human colon cancer cells without or with 5-FU[J].Oncol Lett,2017,13(5):3799-3802.
[21] Liu Q,Chen W,Jiao Y,et al.Pulsatilla saponin A,an active molecule from pulsatilla chinensis,induces cancer cell death and inhibits tumor growth in mouse xenograft models[J].J Surg Res,2014,188(2):387-395.

备注/Memo

备注/Memo:
基金项目:四川省科技厅科研项目(2017SZ0114)
更新日期/Last Update: 2023-08-07