[1]杨 涛,刘 勇,曹兴华,等.三七总皂苷对短暂性前脑缺血大鼠海马区神经元的修复作用实验研究[J].陕西医学杂志,2023,52(7):803-808.[doi:DOI:10.3969/j.issn.1000-7377.2023.07.006]
　YANG Tao,LIU Yong,CAO Xinghua,et al.Repair effect of panax notoginseng total saponins on hippocampal neurons in rats with transient forebrain ischemia[J].,2023,52(7):803-808.[doi:DOI:10.3969/j.issn.1000-7377.2023.07.006]
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三七总皂苷对短暂性前脑缺血大鼠海马区神经元的修复作用实验研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年7期
页码:
803-808
栏目:
基础研究
出版日期:
2023-07-05

文章信息/Info

Title:
Repair effect of panax notoginseng total saponins on hippocampal neurons in rats with transient forebrain ischemia
作者:
杨 涛; 刘 勇; 曹兴华; 梁艳山; 柯雪茹; 陈 杰; 马晓媛
(新疆医科大学附属中医医院手术麻醉科,新疆 乌鲁木齐 830000)
Author(s):
YANG Tao; LIU Yong; CAO Xinghua; LIANG Yanshan; KE Xueru; CHEN Jie; MA Xiaoyuan
(Department of Anesthesiology,Affiliated Hospital of Traditional Chinese Medicine,Xinjiang Medical University,Urumqi 830000,China)
关键词:
三七总皂苷;  短暂性前脑缺血;  海马;  神经元;  修复;  大鼠
Keywords:
Panax notoginseng total saponins;  Transient forebrain ischemia;  Seahorse;  Neuron;  Repair;  Rats
分类号:
R 743
DOI:
DOI:10.3969/j.issn.1000-7377.2023.07.006
文献标志码:
A
摘要:
目的:探讨三七总皂苷对短暂性前脑缺血大鼠海马区神经元的修复作用。方法:选择30只SD大鼠,随机分为三七总皂苷组、模型组、假手术组,每组10只。模型组、三七总皂苷组使用四血管闭塞建立短暂性前脑缺血大鼠动物模型。假手术组手术方式同三七总皂苷组,不做卡环夹闭、电灼永久性闭塞,仅将右侧颈总动脉、颈内动脉、颈外动脉暴露,之后逐层缝合。三七总皂苷组造模后灌胃给予50 mg/kg三七总皂苷,每天2次,每次间隔12 h,模型组、假手术组灌胃给予等量的0.5%羟甲基纤维素钠。对比三组干预后7、14、28 d的海马神经细胞凋亡、新生神经元数量,对比三组大鼠的学习记忆能力,对比三组干预后7、14、28 d时测量大鼠脑梗死体积及含水量,对比三组DCX/NeuN染色阳性的细胞数量。结果: 模型组干预后7、14、28 d的海马神经细胞凋亡明显较三七总皂苷组、假手术组高(均P<0.05); 三七总皂苷组干预后7、14、28 d的海马神经细胞凋亡明显较假手术组高(均P<0.05); 三七总皂苷组干预后7、14、28 d的海马新生神经元明显较模型组、假手术组高(均P<0.05); 干预后7、14、28 d的模型组新生神经元明显较假手术组高(均P<0.05); 三七总皂苷组中,随着干预时间延长,海马神经细胞凋亡明显降低,新生神经元明显升高(均P<0.05)。模型组大鼠的潜伏期、错误次数、第1记忆错误次数、第1天学习错误次数、第5天记忆错误次数、第5天学习错误次数明显较三七总皂苷组、假手术组高(均P<0.05),三七总皂苷组大鼠的潜伏期、错误次数、第1天记忆错误次数、第1天学习错误次数、第5天记忆错误次数、第5天学习错误次数明显较假手术组高(均P<0.05)。三七总皂苷组中,随着干预时间延长,第1天记忆错误次数、第1天学习错误次数、第5天记忆错误次数、第5天学习错误次数明显降低(均P<0.05)。模型组的脑梗死体积、含水量明显较三七总皂苷组、假手术组高(均P<0.05)。三七总皂苷组的脑梗死体积、含水量明显较假手术组高(均P<0.05)。三七总皂苷组中,随着干预时间延长,脑梗死体积、含水量明显降低(均P<0.05)。三七总皂苷组的DCX/NeuN染色阳性细胞数量明显较模型组、假手术组高(均P<0.05),模型组明显较假手术组高(P<0.05)。结论:三七总皂苷可促进短暂性前脑缺血大鼠海马区神经元的修复。
Abstract:
Objective:To investigate the repair effect of panax notoginseng total saponins on hippocampal neurons in rats with transient forebrain ischemia.Methods:A total of 30 SD rats were randomly divided into panax notoginseng total saponins group,model group and sham operation group,with 10 rats in each group.Model group and panax notoginseng total saponin group were treated with four-vessel occlusion to establish the rat model of transient forebrain ischemia.The operation method of the sham operation group was the same as that of the panax notoginseng total saponins group,and the right common carotid artery,internal carotid artery and external carotid artery were exposed and sutured layer by layer instead of clamping or permanent occlusion by electrocauterization.Panax notoginseng total saponins group was given 50 mg/kg panax notoginseng total saponins by intragastric administration,twice a day at an interval of 12 hours.Model group and sham operation group were given the same amount of 0.5% hydroxymethylcellulose sodium by intragastric administration.Hippocampal nerve cell apoptosis and the number of new neurons in the three groups were compared at 7,14 and 28 days after intervention.The learning and memory ability of the three groups were compared.The volume and water content of cerebral infarction in the three groups were compared at 7,14 and 28 days after intervention.The number of DCX/NeuN staining positive cells in the three groups were compared.Results:The apoptosis of hippocampal nerve cells in model group was significantly higher than that in panax notoginseng total saponins group and sham operation group at 7,14 and 28 days after intervention(all P<0.05).The hippocampal nerve cell apoptosis of panax notoginseng total saponins group was significantly higher at 7,14 and 28 days after intervention than that of sham operation group(all P<0.05).The number of hippocampal new neurons in panax notoginseng total saponins group was significantly higher at 7,14 and 28 days after intervention than that in model and sham operation groups(all P<0.05).The number of new neurons in the model group at 7,14 and 28 days after intervention was significantly higher than that in the sham group(all P<0.05).In panax notoginseng total saponins group,with the extension of intervention time,hippocampal nerve cell apoptosis was significantly decreased,and new neurons were significantly increased(all P<0.05).The latency period,the number of errors,the number of memory errors on the 1st day,the number of learning errors on the 1st day,the number of memory errors on the 5th day and the number of learning errors on the 5th day of the model group were significantly higher than those in the panax notoginseng total saponins group and the sham operation group(all P<0.05).The latency period,the number of errors,the number of memory errors on the 1st day,the number of learning errors on the 1st day,the number of memory errors on the 5th day and the number of learning errors on the 5th day of the panax notoginseng total saponins group were significantly higher than those of the sham operation group(all P<0.05).In panax notoginseng total saponins group,with the extension of intervention time,the number of memory errors on the 1st day,the number of learning errors on the 1st day,the number of memory errors on the 5th day and the number of learning errors on the 5th day were significantly decreased(all P<0.05).The volume and water content of cerebral infarction in model group were significantly higher than those in panax notoginseng total saponins group and sham operation group(all P<0.05).The volume and water content of cerebral infarction in panax notoginseng total saponins group were significantly higher than those in sham operation group(all P<0.05).In panax notoginseng total saponins group,the volume and water content of cerebral infarction decreased significantly with the extension of intervention time(all P<0.05).The number of DCX/NeuN staining positive cells in panax notoginseng total saponins group was significantly higher than that in model group and sham group,and that in model group was significantly higher than that in sham group(all P<0.05).Conclusion:Panax notoginseng saponins can promote hippocampal neuron repair in rats with transient forebrain ischemia.

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备注/Memo

备注/Memo:
基金项目:新疆维吾尔自治区自然科学基金资助项目(2022D01C546); 省部共建中亚高发病成因与防治国家重点实验室开放专项课题(SKL-HIDCA-2020-ZY5)
更新日期/Last Update: 2023-07-05