[1]余 亮,郭 建,周国强,等.结肠癌患者血清微小RNA-193a-5p和微小RNA-139-5p表达检测及其对预后的影响[J].陕西医学杂志,2023,52(6):745-749.[doi:DOI:10.3969/j.issn.1000-7377.2023.06.024]
　YU Liang,GUO Jian,ZHOU Guoqiang,et al.Expression of serum miR-193a-5p and miRNA-139-5p in patients with colon cancer and their effects on prognosis[J].,2023,52(6):745-749.[doi:DOI:10.3969/j.issn.1000-7377.2023.06.024]
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结肠癌患者血清微小RNA-193a-5p和微小RNA-139-5p表达检测及其对预后的影响
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年6期
页码:
745-749
栏目:
临床检验
出版日期:
2023-06-05

文章信息/Info

Title:
Expression of serum miR-193a-5p and miRNA-139-5p in patients with colon cancer and their effects on prognosis
作者:
余 亮; 郭 建; 周国强; 石志良; 俞 铖; 周 放
(徐州医科大学附属常熟医院胃肠外科,江苏 常熟 215500)
Author(s):
YU Liang; GUO Jian; ZHOU Guoqiang; SHI Zhiliang; YU Cheng; ZHOU Fang
(Department of Gastrointestinal Surgery,Changshu Hospital Affiliated to Xuzhou Medical University,Changshu 215500,China)
关键词:
结肠癌;  微小RNA-193a-5p;  微小RNA-139-5p;  临床病理特征;  预后
Keywords:
Colon cancer;  miR-193a-5p;  miR-139-5p;  Clinicopathological characteristics;  Prognosis
分类号:
R 735.35
DOI:
DOI:10.3969/j.issn.1000-7377.2023.06.024
文献标志码:
A
摘要:
目的:检测结肠癌患者血清微小RNA-193a-5p(miR-193a-5p)和微小RNA-139-5p(miR-139-5p)表达水平,分析其对预后的影响。方法:选取结肠癌患者109例为结肠癌组,选取同期体检健康者100例为健康组。收集两组临床资料,采用实时荧光定量PCR检测血清miR-193a-5p和miR-139-5p的表达。分析miR-193a-5p和miR-139-5p表达与结肠癌患者临床病理特征的关系。采用 Cox 风险比例模型分析结肠癌患者预后的影响因素。绘制Kaplan-Meier曲线进行生存分析。结果:结肠癌组miR-193a-5p和miR-139-5p表达明显低于健康组(均P<0.001)。结肠癌患者血清miR-193a-5p和miR-139-5p表达与性别、年龄、病理分型、肿瘤部位、肿瘤直径无明显相关性(均P>0.05 ),与临床分期、分化程度、局部浸润深度和淋巴结转移具有明显相关性(均P<0.001)。Cox风险比例模型分析结果显示,临床分期、分化程度、局部浸润深度、淋巴结转移、miR-193a-5p和miR-139-5p是结肠癌患者预后的影响因素(均P<0.05)。miR-193a-5p低表达组患者5年总生存率明显低于miR-193a-5p高表达组,miR-139-5p低表达组患者5年总生存率明显低于miR-139-5p高表达组(均P<0.05)。结论:血清miR-193a-5p和miR-139-5p在结肠癌患者中表达均明显降低,可能在结肠癌中发挥着抑癌基因的作用。检测血清中miR-193a-5p和miR-139-5p的表达水平有助于结肠癌的诊断及预后评估,可能成为结肠癌早期诊断和预后判断的分子标志物。
Abstract:
Objective:To detect the expression levels of serum microRNA-193a-5p(miR-193a-5p)and microRNA-139-5p(miR-139-5p)in patients with colon cancer,and to analyze their effects on prognosis.Methods:A total of 109 patients with colon cancer were selected as the colon cancer group,and 100 healthy people in the same period were selected as the healthy group.The clinical data of the two groups were collected,and the expression levels of serum miR-193a-5p and miR-139-5p were detected by real-time fluorescence quantitative PCR.The relationship between miR-193a-5p,miR-139-5p expression and clinicopathological characteristics of colon cancer patients was analyzed.Cox risk proportional model was used to analyze the prognostic factors of colon cancer patients.Kaplan-Meier curves were drawn for survival analysis.Results:The expression levels of miR-193a-5p and miR-139-5p in the colon cancer group were significantly lower than those in the healthy group(both P<0.001).The expression of serum miR-193a-5p and miR-139-5p in patients with colon cancer were not significantly correlated with gender,age,pathological type,tumor location and tumor diameter(all P>0.05),but were significantly correlated with clinical stage,differentiation degree,local invasion depth and lymph node metastasis(all P<0.001).Cox risk proportional model analysis showed that clinical stage,degree of differentiation,depth of local invasion,lymph node metastasis,miR-193a-5p and miR-139-5p were prognostic factors for colon cancer patients(all P<0.05).The 5-year overall survival rate of patients in the miR-193a-5p low expression group was significantly lower than that in the miR-193a-5p high expression group,and the 5-year overall survival rate of patients in the miR-139-5p low expression group was significantly lower than that in the miR-139-5p high expression group(both P<0.05).Conclusion:The expressions of serum miR-193a-5p and miR-139-5p are significantly decreased in patients with colon cancer,which may play a role as tumor suppressor genes in colon cancer.Detection of serum miR-193a-5p and miR-139-5p expression levels is helpful for the diagnosis and prognosis evaluation of colon cancer,and may become molecular markers for early diagnosis and prognosis of colon cancer.

参考文献/References:

[1] Mark WA.Colon cancer:The road traveled[J].Surgical Oncology Clinics of North America,2018,27(2):15-18.
[2] 董宏丹.黄芩苷对结肠癌HT-29细胞移植瘤小鼠IL-6/STAT3信号转导通路的影响[J].陕西中医,2021,42(6):696-700.
[3] 徐建立,孙艳华,李 明,等.紫草素通过抑制上皮间质转化缓解结肠癌细胞5-氟尿嘧啶耐药[J].陕西中医,2022,43(3):296-300.
[4] 冯忠军,孙 寅,冯彦蕊,等.微小RNA与临床疾病[J].分子诊断与治疗杂志,2013,5(6):427-432.
[5] 赵 旭,仝 聪,李 毅,等.微小RNA-10a联合甲胎蛋白、糖类抗原50、糖类抗原19-9检测在结直肠腺癌早期诊断中的价值[J].陕西医学杂志,2022,51(1):96-99.
[6] Pranzini E,Leo A,Rapizzi E,et al.MiR-210-3p mediates metabolic adaptation and sustains DNA damage repair of resistant colon cancer cells to treatment with 5-fluorouracil[J].Molecular Carcinogenesis,2019,58(12):2181-2192.
[7] 韩安家,黄 艳,来茂德.肿瘤病理诊断规范(结直肠癌)[J].中华病理学杂志,2016,45(12):822-824.
[8] 何 瑜,唐宽平,马晓颖.5-钙黏蛋白、基质金属蛋白酶9在结直肠癌组织中的表达及临床意义[J].陕西医学杂志,2021,50(5):618-621.
[9] Dienstmann R,Mason MJ,Sinicrope FA,et al.Prediction of overall survival in stage Ⅱ and Ⅲ colon cancer beyond TNM system:A retrospective,pooled biomarker study[J].Annals of Oncology,2017,28(5):1023-1031.
[10] Li HE,Chen Y,Wen J,et al.Metastasis-associated in colon cancer 1:A promising biomarker for the metastasis and prognosis of colorectal cancer[J].Oncology Letters,2017,14(4):3899-3908.
[11] 谢国荣,李海玉.结肠癌中差异表达miRNA的筛选及在结肠癌中的临床意义分析[J].医学分子生物学杂志,2021,18(4):261-266.
[12] 林汉利,陈小欢,王晓玲,等.miRNA靶向调控CCR7抑制结肠癌细胞增殖及侵袭的研究[J].国际医药卫生导报,2017,23(24):3832-3835,3839.
[13] 殷玉敬,高 辉,岳 林,等.miR-193a-5p靶向VASP干扰PI3K/AKT信号通路抑制胃癌细胞增殖和诱导细胞凋亡的机制研究[J].解放军医药杂志,2021,33(5):35-42.
[14] Li M,Wu P,Yang Z,et al.MiR-193a-5p promotes pancreatic cancer cell metastasis through SRSF6-mediated alternative splicing of OGDHL and ECM1[J].American Journal of Cancer Research,2020,10(1):38-59.
[15] 石 涛,高 艳,凡 磊,等.miR-139-5p靶向SLC39A7基因通过Wnt/β-catenin信号抑制肾癌细胞周期和增殖[J].中国老年学杂志,2022,42(12):3063-3068.
[16] 王 攀,谢敖文,范钦桥,等.miR-139-5p靶向转化生长因子-β1抑制肝癌细胞侵袭转移的机制[J].中华肝胆外科杂志,2016,22(1):17-23.
[17] Jiao W,Zhang J,Wei Y,et al.MiR-139-5p regulates VEGFR and downstream signaling pathways to inhibit the development of esophageal cancer[J].Digestive and Liver Disease,2018,51(1):149-156.
[18] 贾亚旭,王 成,张春妮.血清miR-193a-3p、miR-337-5p和miR-483-5p在食管鳞状细胞癌诊断和预后中的应用[J].现代检验医学杂志,2017,32(2):5-9.
[19] 李 军,韩 玲,董 力,等.血清miR-221、miR-193a-5p与卵巢上皮癌患者临床病理特征和预后的关系[J].河北医药,2020,42(23):3564-3567.
[20] 崔开颖,赵素芬,朱根海.上皮性卵巢癌组织中miR-21-5p、miR-29-3p、miR-139-5p表达变化及意义[J].山东医药,2017,57(22):65-67.

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备注/Memo

备注/Memo:
基金项目:江苏省卫生健康委员会医学科研立项课题(Z2021032); 江苏省重点实验室开放基金支持项目(XZSYSKF2020027)
更新日期/Last Update: 2023-06-05