[1]王玉玮,李丁新,赵 飞,等.熊果酸调控AMPK/SIRT1通路对牙周炎大鼠牙槽骨吸收的影响[J].陕西医学杂志,2023,52(5):517-522.[doi:DOI:10.3969/j.issn.1000-7377.2023.05.005]
　WANG Yuwei,LI Dingxin,ZHAO Fei,et al.Effects of ursolic acid on alveolar bone resorption in periodontitis rats by regulating AMPK/SIRT1 pathway[J].,2023,52(5):517-522.[doi:DOI:10.3969/j.issn.1000-7377.2023.05.005]
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熊果酸调控AMPK/SIRT1通路对牙周炎大鼠牙槽骨吸收的影响
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年5期
页码:
517-522
栏目:
基础研究
出版日期:
2023-05-05

文章信息/Info

Title:
Effects of ursolic acid on alveolar bone resorption in periodontitis rats by regulating AMPK/SIRT1 pathway
作者:
王玉玮1; 李丁新1; 赵 飞2; 齐宝明1; 高志强1
(1.唐山市协和医院口腔修复科,河北 唐山 063000; 2.唐山市协和医院儿童牙科,河北 唐山 063000)
Author(s):
WANG Yuwei; LI Dingxin; ZHAO Fei; QI Baoming; GAO Zhiqiang
(Department of Prosthodontics,Tangshan Union Medical College Hospital,Tangshan 063000,China)
关键词:
熊果酸;  牙周炎大鼠;  牙槽骨吸收;  牙周组织;  腺苷酸活化蛋白激酶;  沉默信息调节因子1
Keywords:
Ursolic acid;  Periodontitis rat;  Alveolar bone resorption;  Periodontal tissue;  Adenosine monophosphate-activated protein kinase;  Silent information regulator 1
分类号:
R 781.41
DOI:
DOI:10.3969/j.issn.1000-7377.2023.05.005
文献标志码:
A
摘要:
目的:探讨熊果酸(UA)对牙周炎大鼠牙槽骨吸收及腺苷酸活化蛋白激酶/沉默信息调节因子1(AMPK/SIRT1)通路的影响。方法:将40只大鼠分为对照组、模型组、UA组(150 mg/kg)、UA+AMPK抑制剂组(150 mg/kg+0.2 mg/kg); 模型组、UA组、UA+AMPK抑制剂组建立牙周炎大鼠模型,造模成功后各组给予相应处理。连续给药2周后,微计算机断层扫描技术检测大鼠牙槽骨釉牙骨质界至牙槽嵴顶的距离(CEJ-ABC)、骨小梁厚度(Tb.Th)、骨小梁分离度(Tb.Sp)、骨小梁数目(Tb.N); 观察牙周组织病理变化; 对大鼠牙周组织进行破骨细胞计数; 检测大鼠牙周组织核因子κB受体活化因子配体(RANKL)、骨保护素(OPG)、AMPK、磷酸化AMPK(p-AMPK)、SIRT1、乙酰化NF-κB p65(Ac-NF-κB p65)、核因子κB p65(NF-κB p65)、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)蛋白表达。结果:与对照组比较,模型组大鼠牙槽骨表面不平整,牙槽骨高度明显降低,有较多骨吸收陷窝,有大量炎性细胞浸润,牙周膜纤维排列紊乱,牙槽骨CEJ-ABC、Tb.Sp、牙周组织破骨细胞数、RANKL、Ac-NF-κB p65、NF-κB p65、TNF-α、IL-1β蛋白表达水平显著升高(均P<0.05),Tb.N、Tb.Th、牙周组织OPG、p-AMPK/AMPK、SIRT1蛋白表达水平显著降低(均P<0.05)。与模型组比较,UA组大鼠牙周组织病理改变减轻,牙槽骨吸收减少,牙周纤维排列较整齐,炎症细胞浸润程度减轻,牙槽骨CEJ-ABC、Tb.Sp、牙周组织破骨细胞数、RANKL、Ac-NF-κB p65、NF-κB p65、TNF-α、IL-1β蛋白表达水平显著降低(均P<0.05),Tb.N、Tb.Th、牙周组织OPG、p-AMPK/AMPK、SIRT1蛋白表达水平显著升高(均P<0.05); AMPK抑制剂可逆转UA对牙周炎大鼠牙槽骨吸收的抑制作用。结论:UA可通过激活AMPK/SIRT1信号通路,抑制牙周炎大鼠牙槽骨吸收,促进牙槽骨的修复与重建。
Abstract:
Objective:To investigate the effects of ursolic acid(UA)on alveolar bone resorption and adenosine monophosphate-activated protein kinase/silent information regulator 1(AMPK/SIRT1)pathway in periodontitis rats.Methods:Fourty rats were divided into control group,model group,UA group(150 mg/kg),UA+AMPK inhibitor group(150 mg/kg+0.2 mg/kg)and periodontitis rat models were established in model group,UA group and UA+AMPK inhibitor group.After successful modeling,each group was given corresponding treatments.After 2 weeks of continuous administration,the distance from enamelo cemental junction to alveolar bone crest(CEJ-ABC),trabecular bone thickness(Tb.Th),trabecular bone separation(Tb.Sp)and trabecular number(Tb.N)were detected by microcomputer tomography; the pathological changes of periodontal tissue were observed; osteoclasts were counted in the periodontal tissue of rats; the expressions of nuclear factor κB receptor activator ligand(RANKL),osteoprotegerin(OPG),AMPK,phosphorylated AMPK(p-AMPK),SIRT1 and acetylated NF-κB p65(Ac-NF-κB p65),nuclear factor κB p65(NF-κB p65),tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)proteins of rats periodontal tissue were detected.Results:Compared with the control group,the alveolar bone surface of the rats in the model group was uneven,the height of the alveolar bone was obviously reduced,there were more bone resorption pits,a lot of inflammatory cells were infiltrated,the periodontal ligament fibers were disordered,and alveolar bone CEJ-ABC,Tb.Sp,number of osteoclasts in periodontal tissue,expression levels of RANKL,Ac-NF-κB p65/NF-κB p65,TNF-α,IL-1β proteins were significantly increased(all P<0.05),Tb.N,Tb.Th,expression levels of periodontal tissue OPG,p-AMPK/AMPK,SIRT1 proteins were significantly decreased(all P<0.05).Compared with the model group,the periodontal tissue changes in the UA group were reduced,the alveolar bone resorption was reduced,the periodontal fibers were arranged more neatly,the inflammatory cells infiltration were reduced,alveolar bone CEJ-ABC,Tb.Sp,number of osteoclasts in periodontal tissue,expression levels of RANKL,Ac-NF-κB p65/NF-κB p65,TNF-α,IL-1β proteins were significantly decreased(all P<0.05),and Tb.N,Tb.Th,expression levels of periodontal tissue OPG,p-AMPK/AMPK,SIRT1 proteins were significantly increased(all P<0.05).AMPK inhibitor was able to reverse the inhibitory effect of UA on alveolar bone resorption in periodontitis rats.Conclusion:UA can inhibit the resorption of alveolar bone and ameliorate the repair and reconstruction of alveolar bone in rats with periodontitis by activating the AMPK/SIRT1 signaling pathway.

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备注/Memo

备注/Memo:
基金项目:河北省医学科学研究计划项目(20201551)
更新日期/Last Update: 2023-05-05