[1]刘 洁,梅军华,张立成.瑞芬太尼在子宫内膜癌细胞增殖及迁移中的调节机制及对miR-212-3p/LIN28B通路的影响[J].陕西医学杂志,2023,52(5):513-516,522.[doi:DOI:10.3969/j.issn.1000-7377.2023.05.004]
　LIU Jie,MEI Junhua,ZHANG Licheng.Regulatory mechanism of remifentanil in proliferation and migration of endometrial cancer cells and its effect on miR-212-3p/LIN28B pathway[J].,2023,52(5):513-516,522.[doi:DOI:10.3969/j.issn.1000-7377.2023.05.004]
点击复制

瑞芬太尼在子宫内膜癌细胞增殖及迁移中的调节机制及对miR-212-3p/LIN28B通路的影响
分享到：

《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年5期
页码:
513-516,522
栏目:
基础研究
出版日期:
2023-05-05

文章信息/Info

Title:
Regulatory mechanism of remifentanil in proliferation and migration of endometrial cancer cells and its effect on miR-212-3p/LIN28B pathway
作者:
刘 洁; 梅军华; 张立成
(黄冈市中心医院,湖北 黄冈 438000)
Author(s):
LIU Jie; MEI Junhua; ZHANG Licheng
(Huanggang Central Hospital,Huanggang 438000,China)
关键词:
瑞芬太尼;  子宫内膜癌;  增殖及迁移;  miR-212-3p/LIN28B通路;  生物学活性;  微小RNA
Keywords:
Remifentanil;  Endometrial carcinoma;  Proliferation and migration;  miR-212-3p/LIN28B pathway;  Biological activity;  microRNA
分类号:
R 737.33
DOI:
DOI:10.3969/j.issn.1000-7377.2023.05.004
文献标志码:
A
摘要:
目的:探讨子宫内膜癌细胞采用瑞芬太尼的干预效果及对增殖、迁移与miR-212-3p/LIN28B通路的影响。方法:①子宫内膜癌Ishikawa细胞放置在4个试管中,给予瑞芬太尼5、50、500 ng/ml 24 h培养,以瑞芬太尼0 ng/ml为对照组; 采用四甲基偶氮唑蓝(MTT)法检测细胞增殖抑制率,实时荧光PCR法检测miR-212-3p、LIN28B mRNA表达水平,Western blot法检测细胞周期蛋白(Cyclin D1)、基质金属蛋白酶(MMP-2)、基质金属蛋白酶-9(MMP-9)及LIN28B蛋白表达水平,采用Transwell法测定四组细胞迁移及侵袭率。②子宫内膜癌Ishikawa细胞中转染miR-NC、miR-212-3p mimics、si-NC及si-LIN28B,并采用500 ng/ml瑞芬太尼处理,采用上述方法评估细胞的增殖和迁移。结果:四组24 h细胞增殖率比较无统计学差异(均P>0.05); 且细胞增殖抑制率呈瑞芬太尼药物剂量依赖性; 实时荧光PCR法测定结果表明:四组不同浓度瑞芬太尼处理后miR-212-3p mRNA呈上升趋势(均P<0.05),而LIN28B mRNA水平呈下降趋势(P<0.05),并呈剂量依赖性; Western blot结果表明:四组Cyclin D1、MMP-2、MMP-9及LIN28B蛋白表达比较差异具有统计学意义(均P<0.05),随着瑞芬太尼药物剂量增加,其表达水平呈下降趋势; 随着瑞芬太尼浓度增加,迁移细胞数与侵袭细胞数下降明显,并表现出剂量依赖性; miR-NC组细胞增殖抑制率低于miR-212-3p mimics组(P<0.05),迁移细胞数、侵袭细胞数高于miR-212-3p mimics组(均P<0.05); 抑制LIN28B蛋白后si-LIN28B细胞增殖抑制率高于si-NC组(P<0.05),迁移细胞数、侵袭细胞数及LIN28蛋白均低于si-NC(均P<0.05); 瑞芬太尼500 ng/ml+anti-miR-212-3p组miR-212-3p、细胞增殖抑制率低于瑞芬太尼500 ng/ml+anti-miR-NC组(均P<0.05),LIN28B蛋白、迁移细胞数及侵袭细胞数高于瑞芬太尼500 ng/ml+anti-miR-NC组(均P<0.05)。结论:瑞芬太尼能抑制子宫内膜癌细胞生物学活性,可能与药物调控miR-212-3p/LIN28B通路有关。
Abstract:
Objective:To investigate the effects of remifentanil on proliferation,migration and miR-212-3p/LIN28B pathway in endometrial cancer cells.Methods:① Endometrial carcinoma Ishikawa cells were placed in 4 test tubes and cultured with remifentanil 5,50,500 ng/ml for 24 hours.Remifentanil 0 ng/ml was used as control group.The inhibitory rate of cell proliferation was detected by MTT assay,and the mRNA expression levels of miR-212-3p and LIN28B were detected by real-time PCR.The expression levels of Cyclin D1,matrix metalloproteinase(MMP-2),matrix metalloproteinase-9(MMP-9)and LIN28B were detected by Western blot.The migration and invasion rates of the four groups were determined by Transwell method.②The endometrial carcinoma Ishikawa cells were transfected with miR-NC,miR-212-3p mimics,si-NC and si-LIN28B,and treated with 500 ng/ml remifentanil,and the proliferation and migration of the cells were evaluated by the above methods.Results:There was no statistical difference in cell growth rate in 24 hours among the four groups(all P>0.05).The inhibition rate of cell proliferation was dose-dependent on remifentanil.Real-time PCR assay results showed that the mRNA level of miR-212-3p increased and that of LIN28B decreased in a dose-dependent manner after remifentanil treatment(all P<0.05).Western blot results showed that Cyclin D1,MMP-2,MMP-9 and LIN28B proteins in the four groups had statistical significance(all P<0.05),and the expression levels of cyclin D1,MMP-2,MMP-9 and Lin28B proteins decreased with the increase of remifentanil dose.With the increase of remifentanil concentration,the number of migrating cells and invading cells decreased significantly and showed dose dependence.The inhibition rate of cell proliferation in miR-NC group was lower than that in miR-212-3p mimics,and the number of migrating cells and invading cells were higher than those of miR-212-3p mimics group(all P<0.05).The inhibition rate of cell proliferation in si-LIN28B group was higher than that in si-NC group,and the number of migrating cells,invading cells and LIN28 protein were lower than those of si-NC(all P<0.05).The inhibition rates of miR-212-3p and cell proliferation in remifentanil 500 ng/ml+anti-miR-212-3p group were lower than those in remifentanil 500 ng/ml+ anti-miR-212-3p group,and the LIN28B protein,the number of migrated cells and the number of invaded cells were higher than those in remifentanil 500 ng/ml+anti-miR-NC group(all P<0.05).Conclusion:Remifentanil can inhibit the biological activity of endometrial cancer cells,which may be related to the drug regulation of miR-212-3p/LIN28B pathway.

参考文献/References:

[1] 唐婧英,李选发,邓小华,等.瑞芬太尼通过调控miR-212-3p/LIN28B表达影响子宫内膜癌细胞的增殖、迁移和侵袭[J].中国老年学杂志,2021,41(23):5370-5375.
[2] 秦巧红,张 楠,赵书君,等.miR-216a-5p和WASL在子宫内膜癌组织中的表达及其调控子宫内膜癌细胞增殖、迁移和侵袭的分子机制[J].吉林大学学报:医学版,2020,46(4):844-850.
[3] Sugiura H,Nishimori H,Matsuoka H,et al.Successful treatment of acute promyelocytic leukemia complicated with endometrial cancer by arsenic trioxide[J].Acta med Okayama,2021,75(2):219-224.
[4] 肖 庆,刘 莉,胡雅君,等.miR-125b-5p靶向STAT3调控免疫因子IL-6/10的表达抑制子宫内膜癌细胞的增殖、迁移和侵袭的机制研究[J].免疫学杂志,2019,35(7):553-560.
[5] 赵 瑾,胡海燕,周艳红.高迁移率族蛋白B1过表达对子宫内膜癌细胞增殖、周期和凋亡的影响及机制研究[J].陕西医学杂志,2022,51(5):539-542.
[6] John B,Harsh S,Faye E,et al.Cancer prevention with aspirin in hereditary colorectal cancer(Lynch syndrome),10-year follow-up and registry-based 20-year data in the CAPP2 study:A double-blind,randomised,placebo-controlled trial[J].Lancet,2021,395(10240):1855-1863.
[7] 杨槟伊,陈伟明,尉嘉斌,等.miR-92a-3p靶向调控PTEN对神经母细胞瘤细胞增殖、侵袭和迁移的影响[J].中华小儿外科杂志,2022,43(3):257-264.
[8] 高瑞超,李 敏,金朋然,等.恩格列净通过miR-497-3p/GPLD1通路调控高糖诱人肾小球微血管内皮细胞增殖凋亡的机制研究[J].河北医学,2022,28(2):194-201.
[9] 徐 珊,程 珂,刘鹏宇.子宫内膜癌组织血管紧张素(1-7)、miR-106b表达与患者临床病理分期相关性研究[J].陕西医学杂志,2022,51(5):621-624.
[10] Wang Y,Yin L.LINC00461 promoted endometrial carcinoma growth and migration by targeting microRNA-219-5p/cyclooxygenase-2 signaling axis[J].Cell Transplant,2021,30(1):963689721989616.
[11] 乔素青,李 涵,曹 妍,等.miR-144-3p对卵巢癌细胞增殖、迁移和侵袭的影响及机制[J].山东医药,2021,61(7):46-50.
[12] 陆小宇,罗 慧,何 鸳,等.凤香洗液中“丁香-苦参”配伍干预子宫内膜癌的活性成分及作用机制预测分析[J].陕西中医,2021,42(12):1808-1813.
[13] 黄 涛,关德凤,杨永秀.基于PI3K/AKT/NF-κB通路探索异紫堇碱抑制子宫内膜癌细胞系HEC-1B细胞增殖、迁移的分子机制[J].兰州大学学报:医学版,2020,46(1):22-29.
[14] Apa B,Aoc D,Mj E,et al.Incidence and risk factors for lower limb lymphedema associated with endometrial cancer:Results from a prospective,longitudinal cohort study science direct[J].Gynecol Oncol,2020,158(2):375-381.
[15] 孙丽丽,车晓霞,冯炜炜.PKM2促进子宫内膜癌细胞增殖的作用及机制研究[J].现代妇产科进展,2022,31(1):37-40.
[16] 刘晨溪,赵红娟,田 伟,等.miR-199b-3p对肾癌细胞增殖、凋亡、侵袭的影响及其机制探讨[J].山东医药,2022,62(4):26-30,35.
[17] Smith AJ,Applebaum J,Fader AN.The association of the affordable care's medicaid expansion on survival in gynecologic cancer:A national cancer database study[J].J Clin Oncol,2021,39(15):1501-1505.
[18] 向 楠,陈 涛,肖 丽,等.miR-3911对卵巢癌细胞增殖和迁移的影响及其机制[J].山西医科大学学报,2021,52(11):1411-1416.
[19] 夏 莹,周晓莉,刘 杰,等.miR-224-5p靶向调控KIF23通过NF-κB信号通路影响宫颈癌细胞增殖、迁移和侵袭[J].现代肿瘤医学,2020,28(11):1844-1850.
[20] 罗 星,陈 琪.健脾消癌方联合化疗对大肠癌患者肿瘤标志物及预后的影响[J].陕西中医,2022,43(9):1249-1251.

相似文献/References:

[1]区浩林.右美托咪定联合瑞芬太尼TCI清醒镇静镇痛在输尿管镜钬激光碎石术中临床研究*[J].陕西医学杂志,2019,(1):32.
[2]常 艳,冯 硕△.瑞芬太尼静脉自控镇痛在孕产妇分娩中的镇痛效果及对新生儿影响研究*[J].陕西医学杂志,2019,(11):1560.
[3]田 道,张盘石,刘艳霞,等.不同剂量瑞芬太尼联合丙泊酚靶控输注对老年全麻患者应激反应和血流动力学的影响研究*[J].陕西医学杂志,2020,49(7):827.[doi:DOI:10.3969/j.issn.10007377.2020.07.016]
　TIAN Dao,ZHANG Panshi,LIU Yanxia,et al.Effects of different doses of remifentanil combined with propofol target-controlled infusion on stress response and hemodynamics in elderly patients with general anesthesia[J].,2020,49(5):827.[doi:DOI:10.3969/j.issn.10007377.2020.07.016]
[4]赵 瑾,胡海燕,周艳红.高迁移率族蛋白B1过表达对子宫内膜癌细胞增殖、周期和凋亡的影响及机制研究[J].陕西医学杂志,2022,51(5):539.[doi:DOI:10.3969/j.issn.1000-7377.2022.05.006]
　ZHAO Jin,HU Haiyan,ZHOU Yanhong.Effect and mechanism of HMGB1 overexpression on proliferation,cycle and apoptosis of endometrial cancer cells[J].,2022,51(5):539.[doi:DOI:10.3969/j.issn.1000-7377.2022.05.006]
[5]王秋菊,郑丽雅,申 岩,等.微小RNA-335-5p靶向轴突导向因子3B对子宫内膜癌细胞增殖、凋亡的影响[J].陕西医学杂志,2023,52(7):798.[doi:DOI:10.3969/j.issn.1000-7377.2023.07.005]
　WANG Qiuju,ZHENG Liya,SHEN Yan,et al.Effects of miR-335-5p targeting SEMA3B on proliferation and apoptosis of endometrial cancer cells[J].,2023,52(5):798.[doi:DOI:10.3969/j.issn.1000-7377.2023.07.005]
[6]郭 娟,张凤玲,薛 娇,等.醋酸甲羟孕酮联合糖蛋白抑制剂对子宫内膜癌化疗患者肿瘤标志物、免疫系统功能和生存质量的影响[J].陕西医学杂志,2023,52(10):1335.[doi:DOI:10.3969/j.issn.1000-7377.2023.10.011]
　GUO Juan,ZHANG Fengling,XUE Jiao,et al.Effects of medroxyprogesterone acetate combined with glycoprotein inhibitors on tumor markers,immune system function and quality of life in patients with endometrial cancer undergoing chemotherapy[J].,2023,52(5):1335.[doi:DOI:10.3969/j.issn.1000-7377.2023.10.011]

备注/Memo

备注/Memo:
基金项目:湖北省卫生健康委员会科研项目(WJ2019M090)
更新日期/Last Update: 2023-05-05