[1]羡 莹,轩凤娇,杨庆林,等.骨膜蛋白调控卵巢浆液性癌细胞增殖和凋亡实验研究[J].陕西医学杂志,2023,52(4):476-480.[doi:DOI:10.3969/j.issn.1000-7377.2023.04.026]
　XIAN Ying,XUAN Fengjiao,YANG Qinglin,et al.Proliferation and apoptosis regulation by periostin in ovarian serous carcinoma[J].,2023,52(4):476-480.[doi:DOI:10.3969/j.issn.1000-7377.2023.04.026]
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骨膜蛋白调控卵巢浆液性癌细胞增殖和凋亡实验研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年4期
页码:
476-480
栏目:
临床病理
出版日期:
2023-04-05

文章信息/Info

Title:
Proliferation and apoptosis regulation by periostin in ovarian serous carcinoma
作者:
羡 莹1; 轩凤娇2; 杨庆林2; 刘 畅1; 张 林3; 徐诗博4(泰国); 陈 媛1
(1.锦州医科大学附属第三医院妇科,辽宁 锦州 121000; 2.锦州医科大学附属第三医院普外科,辽宁 锦州 121000; 3.锦州医科大学附属第三医院肿瘤科,辽宁 锦州121000; 4.泰国格乐大学教育系,泰国 曼谷 10900)
Author(s):
XIAN Ying; XUAN Fengjiao; YANG Qinglin; LIU Chang; ZHANG Lin; XU Shibo; CHEN Yuan
(Department of Gynecology,the Third Affiliated Hospital of Jinzhou Medical University,Jinzhou 121000,China)
关键词:
卵巢;  浆液性癌;  骨膜蛋白;  临床病理特征;  增殖;  凋亡
Keywords:
Ovary;  Serous carcinoma;  Periostin;  Clinicopathological features;  Proliferation;  Apoptosis
分类号:
R 737.31
DOI:
DOI:10.3969/j.issn.1000-7377.2023.04.026
文献标志码:
A
摘要:
目的:检测卵巢浆液性癌中骨膜蛋白(POSTN)的表达特征,分析其对细胞增殖和凋亡的调控作用。方法:选择41例卵巢浆液性癌患者作为研究对象,选择41例卵巢浆液性囊腺瘤患者作为对照,均留取术后病变组织。应用免疫组化法检测POSTN、增殖细胞核抗原(PCNA)和Bcl-2相关X蛋白(BAX)的表达。选择人卵巢癌细胞株SK-OV-3和OVCAR-3,选择人正常卵巢上皮细胞株IOSE80,基于SK-OV-3和OVCAR-3细胞株转染小干扰RNA-POSTN分别建立si-POSTN组,并设立空白对照组,应用Western blot检测POSTN、PCNA和BAX的表达,应用CCK-8法检测细胞增殖活性,应用流式细胞术观察细胞凋亡。结果:卵巢浆液性癌组织中POSTN、PCNA的阳性率明显高于卵巢浆液性囊腺瘤组织,卵巢浆液性癌组织中BAX的阳性率明显低于卵巢浆液性囊腺瘤组织(均P<0.05)。相关分析显示卵巢浆液性癌组织中POSTN和PCNA呈正相关性(r=0.68,P=0.0241)、POSTN和BAX呈负相关性(r=-0.62,P=0.0348)。SK-OV-3和OVCAR-3细胞株中POSTN的表达均明显高于JOSE80(均P<0.05)。相对于SK-OV-3和OVCAR-3细胞株的空白对照组,si-POSTN组的POSTN、PCNA的表达均下降,BAX的表达均升高,si-POSTN组均在72 h时细胞增殖活性明显下降,并持续至96 h,且细胞凋亡率均升高(均P<0.05)。结论:POSTN在卵巢浆液性癌中高表达,可能通过促进肿瘤细胞增殖、抑制凋亡发挥作用。
Abstract:
Objective:To detect the expression characteristics of Periostin(POSTN)in ovarian serous carcinoma,and to analyze its regulatory effects on cell proliferation and apoptosis.Methods:A total of 41 patients with ovarian serous carcinoma were selected as the study group,and 41 patients with ovarian serous cystadenoma were selected as the control group.The postoperative pathological tissues were collected.The expressions of POSTN,proliferating cell nuclear antigen(PCNA)and Bcl-2 associated X protein(BAX)were detected by immunohistochemistry.Human ovarian cancer cell lines SK-OV-3 and OVCAR-3 and human normal ovarian epithelial cell line IOSE80 were selected.The human ovarian cancer cell lines SK-OV-3 and OVCAR-3 were selected,and the human normal ovarian epithelial cell line IOSE80 was selected,and the si-POSTN group was established based on the transfection of SK-OV-3 and OVCAR-3 cell lines with small interfering RNA-POSTN,and a blank control group was established.Western blot was used to detect the expression of POSTN,PCNA and BAX.CCK-8 method was used to detect the cell proliferation activity,and flow cytometry was used to observe the cell apoptosis.Results:The positive rates of POSTN and PCNA were significantly higher in ovarian serous carcinoma than those in ovarian plasmacytoma tissues,and the positive rate of BAX was significantly lower in ovarian serous carcinoma than that in ovarian plasmacytoma tissues(all P<0.05).Positive correlation was found between POSTN and PCNA(r=0.68,P=0.0241),negative correlation was found between POSTN and BAX(r=-0.62,P=0.0348)in ovarian serous carcinoma.The expression of POSTN was significantly higher in SK-OV-3 and OVCAR-3 cell lines than that in JOSE80(all P<0.05).Relative to the blank control group of SK-OV-3 and OVCAR-3 cell lines,the expression of POSTN and PCNA decreased and the expression of BAX increased,cell proliferative activity decreased at 72 hours and continued until 96 hours,apoptosis rate increased in the si-POSTN group(all P<0.05).Conclusion:POSTN is highly expressed in ovarian serous cancer and may play a role in promoting tumor cell proliferation and inhibiting apoptosis.

参考文献/References:

[1] 段小霞,郭 华,付 婷,等.震颤同系物-5过表达对卵巢癌细胞A2780增殖和凋亡的影响[J].陕西医学杂志,2022,51(5):529-533.
[2] 朱军义,马繁华,徐亚辉.龙葵碱调控p-AKT、Cleaved caspase-3和p53蛋白表达抑制卵巢癌细胞增殖实验研究[J].陕西中医,2019,40(5):556-560.
[3] 王 巍,李顺双,刘 刚.SETDB1基因在卵巢高级别浆液性癌组织中的表达及对卵巢上皮性癌细胞增殖和侵袭能力的影响[J].中华妇产科杂志,2022,57(4):293-300.
[4] Jia YY,Yu Y,Li HJ.POSTN promotes proliferation and epithelial-mesenchymal transition in renal cell carcinoma through ILK/AKT/mTOR pathway[J].J Cancer,2021,12(14):4183-4195.
[5] Gao F,Liu J,Gan H.The expression of POSTN and immune cell infiltration are prognostic factors of lung adenocarcinoma[J].Medicine(Baltimore),2022,101(34):e30187.
[6] Lv Y,Wang W,Jia WD,et al.High preoparative levels of serum periostin are associated with poor prognosis in patients with hepatocellular carcinoma after hepatectomy[J].Eur J Surg Oncol,2013,39(10):1129-1135.
[7] Yue H,Li W,Chen R,et al.Stromal POSTN induced by TGF-β1 facilitates the migration and invasion of ovarian cancer[J].Gynecol Oncol,2021,160(2):530-538.
[8] 顾玉龙,高 波.肺腺癌患者支气管肺泡灌洗液中POSTN检测的意义[J].华南国防医学杂志,2022,36(8):628-631.
[9] Karlan BY,Dering J,Walsh C,et al.POSTN/TGFBI-associated stromal signature predicts poor prognosis in serous epithelial ovarian cancer[J].Gynecol Oncol,2014,132(2):334-342.
[10] 黄 琳,张志伟,肖 娟,等.POSTN蛋白在胃黏膜癌变过程中的表达及意义[J].诊断病理学杂志,2015,22(9):558-561.
[11] You S,Yoo SA,Choi S,et al.Identification of key regulators for the migration and invasion of rheumatoid synoviocytes through a systems approach[J].Proc Natl Acad Sci USA,2014,111(1):550-555.
[12] Zhu M,Fejzo MS,Anderson L,et al.Periostin promotes ovarian cancer angiogenesis and metastasis[J].Gynecol Oncol,2010,119(2):337-344.
[13] Cheon DJ,Orsulic S.Ten-gene biomarker panel:A new hope for ovarian cancer[J].Biomark Med,2014,8(4):523-526.
[14] Tang M,Liu B,Bu X,et al.Cross-talk between ovarian cancer cells and macrophages through periostin promotes macrophage recruitment[J].Cancer Sci,2018,109(5):1309-1318.
[15] 刘爱东,刘时飞,熊光宜,等.真核翻译起始因子2α促进结直肠腺癌细胞增殖[J].中华实验外科杂志,2022,39(9):1752-1754.
[16] Akinjiyan FA,Dave RM,Alpert E,et al.DDR2 expression in cancer-associated fibroblasts promotes ovarian cancer tumor invasion and metastasis through periostin-ITGB1[J].Cancers(Basel),2022,14(14):3482-3483.
[17] 何 瑜,唐宽平,马晓颖.5-钙黏蛋白、基质金属蛋白酶9在结直肠癌组织中的表达及临床意义[J].陕西医学杂志,2021,50(5):618-621.
[18] 王 莉.卵巢浆液性癌组织中起始因子EIF5A2和基质金属蛋白酶-21的表达及临床意义[J].陕西医学杂志,2020,49(4):411-414.
[19] 叶其淼,李建强.基质金属蛋白酶9在非小细胞肺癌中的研究进展[J].中国医药,2023,18(1):131-134.
[20] 刘爱东,张 爽,王丹琳,等.MMP-19在胃腺癌中的表达及与神经型钙黏素的相关性[J].重庆医科大学学报,2022,47(2):145-150.
[21] Sung PL,Jan YH,Lin SC,et al.Periostin in tumor microenvironment is associated with poor prognosis and platinum resistance in epithelial ovarian carcinoma[J].Oncotarget,2016,7(4):4036-4047.

备注/Memo

备注/Memo:
基金项目:辽宁省科学技术厅科研项目(20170540334)
更新日期/Last Update: 2023-04-06