[1]刘彤崴,田 蕾,任恒杰,等.胃癌中微小RNA-424-5p与真核翻译起始因子5A的表达特征研究[J].陕西医学杂志,2023,52(4):472-475.[doi:DOI:10.3969/j.issn.1000-7377.2023.04.025]
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胃癌中微小RNA-424-5p与真核翻译起始因子5A的表达特征研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年4期
页码:
472-475
栏目:
临床病理
出版日期:
2023-04-05

文章信息/Info

作者:
刘彤崴; 田 蕾; 任恒杰; 周 盾; 王 莹
(锦州医科大学附属第一医院,辽宁 锦州 121000)
关键词:
胃癌;  微小RNA-424-5p;  真核翻译起始因子5A;  靶向关系;  预后
分类号:
R 735.2
DOI:
DOI:10.3969/j.issn.1000-7377.2023.04.025
文献标志码:
A
摘要:
目的:观察胃癌中微小RNA-424-5p(miR-424-5p)与真核翻译起始因子5A(eIF5A)的表达特征,分析二者的靶向关系。方法:收集确诊为胃癌并行根治术的患者共65例作为研究对象,留取肿瘤组织和正常胃黏膜组织,应用实时荧光定量PCR法(qPCR)检测miR-424-5p的表达,应用免疫组化法检测eIF5A的表达。选择胃癌MGC-803细胞株,应用双荧光素酶报告基因实验观察miR-424-5p与eIF5A的结合情况。结果:胃癌中miR-424-5p的表达量明显低于正常胃黏膜组织(P<0.05),eIF5A的阳性率明显高于正常胃黏膜组织(P<0.05),miR-424-5p与eIF5A在不同肿瘤最大径、不同浸润深度、有无淋巴结转移分组的比较中有统计学差异(P<0.05)。miR-424-5p与eIF5A呈负相关性(Y=-2.572X+7.662,P<0.05)。miR-424-5p和eIF5A的表达与术后生存时间相关(均P<0.05)。双荧光素酶报告基因实验显示miR-424-5p与eIF5A具有靶向关系。结论:胃癌组织中miR-424-5p表达下调、eIF5A表达升高,且具有靶向负调控关系。miR-424-5p和eIF5A的表达可能与患者术后生存时间有关。

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备注/Memo

备注/Memo:
基金项目:辽宁省教育厅科学技术研究项目(JYTJCZR 2020052)
更新日期/Last Update: 2023-04-06