[1]周海媚,刘 维.LINC00982在肺腺癌中的表达及功能研究[J].陕西医学杂志,2023,52(4):380-384.[doi:DOI:10.3969/j.issn.1000-7377.2023.04.004]
　ZHOU Haimei,LIU Wei.Expression and function of LINC00982 in lung adenocarcinoma[J].,2023,52(4):380-384.[doi:DOI:10.3969/j.issn.1000-7377.2023.04.004]
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LINC00982在肺腺癌中的表达及功能研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年4期
页码:
380-384
栏目:
基础研究
出版日期:
2023-04-05

文章信息/Info

Title:
Expression and function of LINC00982 in lung adenocarcinoma
作者:
周海媚; 刘 维
(锦州医科大学附属第一医院肿瘤科,辽宁 锦州 121001)
Author(s):
ZHOU Haimei; LIU Wei
(Department of Oncology,the First Affiliated Hospital of Jinzhou Medical University,Jinzhou 121001,China)
关键词:
LINC00982;  肺腺癌;  细胞增殖;  细胞侵袭;  细胞转移
Keywords:
LINC00982;  Lung adenocarcinoma;  Cell proliferation;  Cell invasion;  Cell metastasis
分类号:
R 734.2
DOI:
DOI:10.3969/j.issn.1000-7377.2023.04.004
文献标志码:
A
摘要:
目的:探讨LINC00982在肺腺癌中的表达及功能研究。方法:采用在线数据库分析 LINC00982在肺腺癌组织中的表达情况,并分析LINC00982与肺腺癌患者总生存期(OS)和无病生存期(DFS)的关系。采用A549细胞构建体外模型,并构建LINC00982过表达和正常表达模型。并采用CCK-8实验、Transwell侵袭和转移实验观察细胞的增殖、侵袭和转移情况。结果:与空白对照组相比,LINC00982过表达组的LINC00982相对表达量更高(P<0.05),LINC00982过表达组24、28 hOD值更低(均P<0.05),LINC00982过表达组的细胞侵袭率、迁移率更低(均P<0.05); LINC00982在肺腺癌患者组织中表达降低(P<0.05); LINC00982表达对肺腺癌患者的OS有统计学差异,且LINC00982低表达患者预后生存期更短(P<0.05),对DFS无统计学差异(P>0.05)。结论:LINC00982的表达降低可促进肺腺癌细胞的增殖、迁移和侵袭,而这种促进作用在LINC00982过表达模型中被抑制。临床早期可通过监测患者LINC00982的表达情况对其预后做出预测评估,进而为临床早期治疗策略的介入和调整提供信息参考。
Abstract:
Objective:To investigate the expression and function of LINC00982 in lung adenocarcinoma.Methods:The expression of LINC00982 in lung adenocarcinoma tissues was analyzed by online database,and the relationship between LINC00982 and overall survival(OS),disease-free survival(DFS)of lung adenocarcinoma patients was analyzed.A549 cells were used to construct in vitro model,and LINC00982 overexpression and normal expression models were constructed.The cell proliferation,invasion and metastasis were observed by CCK-8 assay and Transwell assay.Results:Compared with blank control group,the relative expression level of LINC00982 in LINC00982 overexpression group was higher(P<0.05); the 24-hour and 28-hour OD values in LINC00982 overexpression group were lower(all P<0.05); the cell invasion rate and migration rate in LINC00982 overexpression group were lower(all P<0.05); the expression of LINC00982 in lung adenocarcinoma patients was decreased(P<0.05); the expression of LINC00982 had a significant difference in OS of lung adenocarcinoma patients,and the prognosis of patients with low expression of LINC00982 was shorter(P<0.05),but there was no significant difference in DFS(P>0.05).Conclusion:Decreased expression of LINC00982 can promote the proliferation,migration and invasion of lung adenocarcinoma cells,and this promotion effect is inhibited in the overexpression model of LINC00982.In the early clinical stage,the prognosis can be predicted and evaluated by monitoring the expression of LINC00982 in patients,so as to provide information reference for the intervention and adjustment of early clinical treatment strategies.

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备注/Memo

备注/Memo:
基金项目:辽宁省科学技术厅科研项目[辽科发(2018)25号]
更新日期/Last Update: 2023-04-06