[1]王媛媛,朱 静,刘娜娜,等.溶质载体家族1成员5及谷氨酰胺酶2对人甲状腺癌细胞体外恶性行为的影响及机制研究[J].陕西医学杂志,2023,52(3):344-348.[doi:DOI:10.3969/j.issn.1000-7377.2023.03.023]
 WANG Yuanyuan,ZHU Jing,LIU Nana,et al.Effect of solute carrier family 1 member 5 and glutaminases 2 on malignant behavior of human thyroid cancer cells in vitro and its mechanism[J].,2023,52(3):344-348.[doi:DOI:10.3969/j.issn.1000-7377.2023.03.023]
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溶质载体家族1成员5及谷氨酰胺酶2对人甲状腺癌细胞体外恶性行为的影响及机制研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年3期
页码:
344-348
栏目:
临床病理
出版日期:
2023-03-05

文章信息/Info

Title:
Effect of solute carrier family 1 member 5 and glutaminases 2 on malignant behavior of human thyroid cancer cells in vitro and its mechanism
作者:
王媛媛1朱 静2刘娜娜3李生香3王 荣3
(1.榆林市第一医院内分泌科,陕西 榆林 719000; 2.榆林市第一医院病理科,陕西 榆林 719000; 3.榆林市第一医院全科医学科,陕西 榆林 719000)
Author(s):
WANG YuanyuanZHU JingLIU NanaLI ShengxiangWANG Rong
(Department of Endocrinology,the First Hospital of Yulin,Yulin 719000,China)
关键词:
甲状腺癌 谷氨酰胺代谢 溶质载体家族1成员5 谷氨酰胺酶2 体外增殖
Keywords:
Thyroid cancer Glutamine metabolism Solute carrier family 1member 5 Glutaminases 2 Proliferation in vitro
分类号:
R 736.1
DOI:
DOI:10.3969/j.issn.1000-7377.2023.03.023
文献标志码:
A
摘要:
目的:探讨谷氨酰胺(Gln)代谢酶溶质载体家族1成员5(SLC1A5)及谷氨酰胺酶2(GLS2)对人甲状腺癌细胞体外恶性行为的影响及机制。方法:敲减人甲状腺癌细胞系8505C和8305C细胞中SLC1A5及GLS2 mRNA表达。通过噻唑蓝(MTT)、平板克隆形成及Transwell实验评估SLC1A5和GLS2对人甲状腺癌细胞体外恶性行为的影响。观察SLC1A5、GLS2抑制剂对甲状腺癌细胞体外增殖能力及c-myc蛋白表达的影响。结果:敲减人甲状腺癌8505C和8305C细胞中SLC1A5、GLS2 mRNA表达后,细胞体外增殖、克隆形成及迁移能力较对照组明显降低(均P<0.05)。8505C、8305C细胞分别加入抑制剂GPNA和CB-839处理后,与未加抑制剂细胞比较,细胞体外增殖能力和c-myc蛋白表达明显降低(均P<0.05)。结论:下调SLC1A5和GLS2表达可有效抑制甲状腺癌8505C、8305C细胞的体外增殖、克隆形成及迁移能力。SLC1A5、GLS2靶向抑制剂可通过下调c-myc癌基因发挥其抗甲状腺癌细胞生物活性的作用。靶向Gln代谢可能成为甲状腺癌的潜在治疗策略。
Abstract:
Objective:To investigate the effect of glutamine(Gln)metabolic enzymes solute carrier family 1 member 5(SLC1A5)and glutaminases 2(GLS2)on malignant behavior of human thyroid cancer cells in vitro and its mechanism.Methods:The expression of SLC1A5 and GLS2 mRNA was knocked down in human thyroid carcinoma cell lines 8505C and 8305C.The effect of SLC1A5 and GLS2 on malignant behavior in human thyroid cancer cells in vitro was evaluated by MTT,plate plate clonal formation,and Transwell assays.The effect of SLC1A5 and GLS2 inhibitors on the proliferative capacity and c-myc protein expression in thyroid cancer cells in vitro was investigated.Results:Knockdown of SLC1A5 and GLS2 mRNA expression in human thyroid cancer 8505C and 8305C cells resulted in decreased cell proliferation,clonal formation,and migration in vitro compared with control group(all P<0.05).In vitro proliferative capacity and c-myc protein expression were significantly reduced in 8505C and 8305C cells when treated with the inhibitors GPNA and CB-839,respectively(all P<0.05).Conclusion:Down-regulation of SLC1A5 and GLS2 expression is effective in suppressing the proliferation,clonal formation,and migration of human thyroid cancer 8505C and 8305C cells in vitro.Targeting inhibitors of SLC1A5 and GLS2 exert their antithyroid carcinoma cell biological activity by downregulating the c-myc oncogene.Targeting Gln metabolism may be a potential therapeutic strategy for thyroid cancer.

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备注/Memo

备注/Memo:
基金项目:陕西省榆林市科技计划项目(2019-185-32)
更新日期/Last Update: 2023-03-06