[1]吴 冰,王 轩.基于Notch信号通路探讨枸杞多糖对糖尿病肾病大鼠肾纤维化的改善作用[J].陕西医学杂志,2023,52(3):267-272.[doi:DOI:10.3969/j.issn.1000-7377.2023.03.005]
　WU Bing,WANG Xuan.Improvement effect of lycium barbarum polysaccharide on renal fibrosis in diabetic nephropathy rats based on Notch pathway[J].,2023,52(3):267-272.[doi:DOI:10.3969/j.issn.1000-7377.2023.03.005]
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基于Notch信号通路探讨枸杞多糖对糖尿病肾病大鼠肾纤维化的改善作用
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年3期
页码:
267-272
栏目:
基础研究
出版日期:
2023-03-05

文章信息/Info

Title:
Improvement effect of lycium barbarum polysaccharide on renal fibrosis in diabetic nephropathy rats based on Notch pathway
作者:
吴 冰1; 王 轩2
(1.辽宁省金秋医院检验科,辽宁 沈阳 110016; 2.大连市中心医院检验科,辽宁 大连 116033)
Author(s):
WU Bing; WANG Xuan
(Department of Clinical Laboratory,Liaoning Jinqiu Hospital,Shenyang 110016,China)
关键词:
糖尿病肾病;  肾纤维化;  枸杞多糖;  Notch信号通路;  肾功能;  大鼠
Keywords:
Diabetic nephropathy;  Renal fibrosis;  Lycium barbarum polysaccharide;  Notch signaling pathway;  Renal function;  Rats
分类号:
R 587.2
DOI:
DOI:10.3969/j.issn.1000-7377.2023.03.005
文献标志码:
A
摘要:
目的:探究枸杞多糖(LBP)对糖尿病肾病(DN)大鼠肾纤维化的改善作用,并初步探究其可能的作用机制。方法:将SD大鼠分为对照组、模型组(DN组)、LBP组、Notch1抑制剂组(FLI-06组)、LBP+FLI-06组,每组10只。采用全自动生化分析仪对空腹血糖(FBG)和肾功能指标[血尿素氮(BUN)、血肌酐(Scr)、24 h尿微量白蛋白(UMA)]水平。采用放射免疫法检测血清纤维化相关因子[透明质酸(HA)、层粘连蛋白(LN)、Ⅲ型前胶原(PCⅢ)、Ⅳ型胶原(C-Ⅳ)]水平。采用HE染色、Masson染色、透射电镜观察大鼠肾组织形态结构变化。采用免疫印迹和免疫组化法检测肾组织Notch信号通路相关蛋白(Notch1、Jagged1、Hes1)表达水平及阳性表达情况。结果:与对照组比较,其他四组FBG、BUN、Scr、24 h尿UMA、HA、LN、PCⅢ、C-Ⅳ水平升高,肾组织损伤评分、胶原纤维比例升高,Notch1、Jagged1、Hes1蛋白表达水平及阳性表达率升高(均P<0.05)。与DN组比较,LBP组、FLI-06组、LBP+FLI-06组FBG、BUN、Scr、24 h尿UMA、HA、LN、PCⅢ、C-Ⅳ水平降低,肾组织损伤评分、胶原纤维比例降低,Notch1、Jagged1、Hes1蛋白表达水平及阳性表达率下降(均P<0.05)。LBP+FLI-06组FBG、BUN、Scr、24 h尿UMA、HA、LN、PCⅢ、C-Ⅳ水平,肾组织损伤评分,胶原纤维比例,以及Notch1、Jagged1、Hes1蛋白表达水平及阳性表达率低于LBP组和FLI-06组(均P<0.05)。HE染色、Masson染色和透射电镜检查结果表明,DN组肾组织相对于对照组出现病理损伤,纤维化增多,LBP组、FLI-06组、LBP+FLI-06组肾组织病理损伤情况均得到改善,其中LBP+FLI-06组改善最为显著。结论:LBP可改善DN大鼠肾纤维化,其机制可能与抑制Notch信号通路活化有关。
Abstract:
Objective:To explore the improvement effect of lycium barbarum polysaccharide(LBP)on renal fibrosis in diabetic nephropathy(DN)rats based on Notch pathway.Methods:SD rats were divided into control group,model group(DN group),LBP group,Notch1 inhibitor group(FLI-06 group),LBP+FLI-06 group,with 10 rats in each group.The levels of fasting blood glucose(FBG)and renal function indexes [blood urea nitrogen(BUN),serum creatinine(Scr),24-hour urinary microalbumin(UMA)] were measured by automatic biochemical analyzer.The levels of serum fibrosis-related factors [hyaluronic acid(HA),laminin(LN),type Ⅲ procollagen(PCⅢ),and type Ⅳ collagen(C-Ⅳ)] were detected by radioimmunoassay.HE staining,Masson staining and transmission electron microscopy were used to observe the morphological changes of renal tissue.Western blot and immunohistochemistry were used to detect the expression levels and positive expressions of Notch pathway-related proteins(Notch1,Jagged1,Hes1)in renal tissues.Results:Compared with the control group,the levels of FBG,BUN,Scr,UMA,HA,LN,PCⅢ,C-Ⅳ,renal tissue injury score,collagen fiber proportion,Notch1,Jagged1,Hes1 protein expression levels and positive expression rates were increased in the other four groups(all P<0.05).Compared with DN group,the levels of FBG,BUN,Scr,UMA,HA,LN,PCⅢ and C-Ⅳ in LBP group,FLI-06 group and LBP+FLI-06 group were decreased,and the renal tissue damage score and the proportion of collagen fibers were decreased,and the protein expression levels and positive expression rates of Notch1,Jagged1 and Hes1 were decreased(all P<0.05).Compared with the LBP group and the FLI-06 group,the LBP+FLI-06 group had significantly lower levels of FBG,BUN,Scr,UMA,HA,LN,PCⅢ,C-Ⅳ,renal tissue damage score,collagen fiber proportion,and protein expression levels and positive expression rates of Notch1,Jagged1,Hes1(all P<0.05).The results of HE staining,Masson staining and transmission electron microscopy showed that the DN group had pathological damage and increased fibrosis compared with the control group,and the LBP group,FLI-06 group and LBP+FLI-06 group had improved pathological damage of renal tissue,and the LBP+FLI-06 group had the most significant improvement.Conclusion:Lycium barbarum polysaccharide can improve renal fibrosis in DN rats.The mechanism may be related to inhibition of the activation of Notch signaling pathway.

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备注/Memo

备注/Memo:
基金项目:辽宁省大连市医学科学研究计划项目(1811011)
更新日期/Last Update: 2023-03-06