[1]吴晋周,靳建旭.紫草素诱导慢性髓系白血病细胞增殖抑制和凋亡的PTEN信号通路研究[J].陕西医学杂志,2023,52(2):136-140.[doi:DOI:10.3969/j.issn.1000-7377.2023.02.004]
　WU Jinzhou,JIN Jianxu.PTEN signaling pathway of shikonin induced apoptosis and proliferation inhibition in chronic myeloid leukemia cells[J].,2023,52(2):136-140.[doi:DOI:10.3969/j.issn.1000-7377.2023.02.004]
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紫草素诱导慢性髓系白血病细胞增殖抑制和凋亡的PTEN信号通路研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年2期
页码:
136-140
栏目:
基础研究
出版日期:
2023-02-05

文章信息/Info

Title:
PTEN signaling pathway of shikonin induced apoptosis and proliferation inhibition in chronic myeloid leukemia cells
作者:
吴晋周; 靳建旭
(西安市中医医院肿瘤科,陕西 西安 710021)
Author(s):
WU Jinzhou; JIN Jianxu
(Department of Oncology,Xi'an Traditional Chinese Medicine Hospital,Xi'an 710021,China)
关键词:
紫草素;  慢性髓系白血病;  细胞凋亡;  细胞增殖;  增殖抑制;  PTEN信号通路
Keywords:
Shikonin;  Chronic myeloid leukemia;  Apoptosis;  Cell proliferation;  Proliferation inhibition;  PTEN signaling pathway
分类号:
R 733.72
DOI:
DOI:10.3969/j.issn.1000-7377.2023.02.004
文献标志码:
A
摘要:
目的:探讨紫草素对慢性髓系白血病K562细胞的作用及其可能机制。方法:体外培养慢性髓系白血病K562细胞,分组为对照组和紫草素10 μmol/L组、紫草素30 μmol/L组、紫草素50 μmol/L组,分别培养12、24、48、72 h后,MTT法检测细胞增殖抑制率,Annexin V FITC/PI染色结合流式细胞仪检测细胞凋亡率,同时用MTT法观察紫草素对K562细胞生长的影响; Western blot检测细胞内PTEN、p-PI3K和p-Akt蛋白表达。结果:紫草素能有效抑制K562细胞增殖,促进细胞凋亡,上调细胞PTEN蛋白表达量,下调p-PI3K和p-Akt蛋白表达量,各浓度间差异具有统计学意义(均P<0.05)。结论:紫草素能通过调控PTEN、p-PI3K、p-Akt信号通路抑制K562细胞的增殖,促进其凋亡。
Abstract:
Objective:To study the effect of shikonin on K562 cells and its possible mechanism.Methods:K562 cells were cultured in vitro and divided into control group,shikonin 10 μmol/L group,shikonin 30 μmol/L group and shikonin 50 μmol/L group.After 12,24,48 and 72 hours of culture,the inhibition rate of cell proliferation was detected by MTT assay.The apoptosis rate of K562 cells was detected by Annexin V FITC/PI staining combined with flow cytometry,and the effect of shikonin on the growth of K562 cells was observed by MTT assay.The protein expressions of PTEN,p-PI3K and p-Akt were detected by Western blot.Results:Shikonin could effectively inhibit the proliferation of K562 cells,promote the apoptosis of cells,up-regulate the protein expression of PTEN,down-regulate the protein expression of p-Pi3k and p-Akt,with statistically significant differences between different concentrations(all P<0.05).Conclusion:Shikonin can inhibit the proliferation and promote the apoptosis of AK562 cells by regulating PTEN,p-Pi3k,p-Akt signaling pathway.

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更新日期/Last Update: 2023-02-06