[1]刘艳丽,董文婷,尚福军,等.葛根素抑制内质网应激减轻压力负荷引起小鼠心肌肥厚实验研究[J].陕西医学杂志,2023,52(2):123-127.[doi:DOI:10.3969/j.issn.1000-7377.2023.02.001]
　LIU Yanli,DONG Wenting,SHANG Fujun,et al.Puerarin mitigates pressure overload-induced cardiac hypertrophy by inhibiting endoplasmic reticulum stress in mice[J].,2023,52(2):123-127.[doi:DOI:10.3969/j.issn.1000-7377.2023.02.001]
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葛根素抑制内质网应激减轻压力负荷引起小鼠心肌肥厚实验研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年2期
页码:
123-127
栏目:
基础研究
出版日期:
2023-02-05

文章信息/Info

Title:
Puerarin mitigates pressure overload-induced cardiac hypertrophy by inhibiting endoplasmic reticulum stress in mice
作者:
刘艳丽1; 董文婷1; 尚福军1; 冯海涛1; 郭 锦1; 王 锦2
(1.西安国际医学中心医院,陕西 西安 710100; 2.西安市临潼区妇幼保健院,陕西 西安710600)
Author(s):
LIU Yanli; DONG Wenting; SHANG Fujun; FENG Haitao; GUO Jin; WANG Jin
(Xi'an International Medical Center Hospital,Xi'an 710100,China)
关键词:
葛根素;  腹主动脉缩窄;  内质网应激;  小鼠;  心肌肥厚;  纤维化
Keywords:
Puerarin;  Abdominal aorta coarctation;  Endoplasmic reticulum stress;  Mice;  Cardiac hypertrophy;  Fibrosis
分类号:
R 541
DOI:
DOI:10.3969/j.issn.1000-7377.2023.02.001
文献标志码:
A
摘要:
目的:探讨葛根素(Pue)抑制内质网应激(ERS)对压力负荷下小鼠心肌肥厚的作用。方法:采用腹主动脉缩窄术(AAC)模拟压力负荷引起的心肌肥厚,将C57/bl小鼠随机分为四组(n=15):假手术组(Sham组)、葛根素对照组(Pue组)、腹主动脉缩窄术组(AAC组)和葛根素治疗AAC组(AAC+Pue组)。Pue组和AAC+Pue组术后腹腔注射50 mg/(kg·d)Pue,Sham组和AAC组腹腔注射等量的0.9%氯化钠溶液,持续4周后,超声检测左室后壁厚度(LVPWd)、左室内径(LVId)和室间隔厚度(IVSd); 测量心脏重量与体重比(HW/BW),WGA染色观察心肌细胞横截面积,Masson染色观察纤维化程度; RT-PCR检测ANP和Myh7的mRNA水平; Western blot检测PERK、CHOP和GRP78蛋白的表达。结果:Sham组和Pue组各指标间比较无统计学差异(均P>0.05); 和Sham组相比,AAC组LVPWd、LVId和IVSd值增加(均P<0.01),HW/BW比增大,心肌横截面积和纤维化程度增加,ANP和Myh7的mRNA水平增加(均P<0.01),PERK和CHOP蛋白的表达增多,而GRP78表达降低(均P<0.01); 和AAC组相比,AAC+Pue组LVPWd、LVId和IVSd值降低(均P<0.01),HW/BW比减小,心肌横截面积和纤维化程度降低,ANP和Myh7的mRNA水平减少(均P<0.01),PERK和CHOP蛋白的表达降低,而GRP78表达增加(均P<0.01)。结论:Pue可通过抑制内质网应激减轻压力负荷引起的心肌肥厚。
Abstract:
Objective:To investigate the role of puerarin(Pue)in pressure overload-induced cardiac hypertrophy by inhibiting endoplasmic reticulum stress(ERS)in mice.Methods:Pressure overload-induced cardiac hypertrophy model was generated by using abdominal aortic coarctation(AAC)surgery.C57/bl mice were randomly divided into four groups with 15 mice in each:sham group(Sham group),Pue group(Pue group),abdominal aortic coarctation group(AAC group)and AAC treated with Pue group(AAC+Pue group).Pue group and AAC+Pue group were intraperitoneally injected with 50 mg/(kg·d)Pue,Sham group and AAC group were intraperitoneally injected with 0.9%sodium chloride solution.After 4 weeks,left ventricular posterior wall thickness(LVPWd),left ventricular inner diameter(LVId)and ventricular septal thickness(IVSd)were measured by ultrasound; the heart weight to body weight ratio(HW/BW)was measured,the cross-sectional area of cardiomyocytes was observed by WGA staining,and the degree of fibrosis was observed by Masson staining; the mRNA levels of ANP and Myh7 were detected by RT-PCR; the expressions of PERK,CHOP and GRP78 proteins were detected by Western blot.Results:There was no significant difference between sham group and pue group(all P>0.05).Compared with Sham group,the AAC group had increased LVPWd,LVId and IVSd values,increased HW/BW ratio,increased myocardial cross-sectional area and degree of fibrosis,increased ANP and Myh7 mRNA levels,increased expression of PERK and CHOP proteins,and decreased GRP78 expression(all P<0.01).Compared with AAC group,the AAC Pue group had lower LVPWd,LVId and IVSd values,smaller HW/BW ratio,decreased myocardial cross-sectional area and degree of fibrosis,decreased ANP and Myh7 mRNA levels,decreased expression of PERK and CHOP proteins,and increased GRP78 expression(all P<0.01).Conclusion:Pue can alleviate pressure overload-induced cardiac hypertrophy by inhibiting endoplasmic reticulum stress.

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备注/Memo

备注/Memo:
基金项目:陕西省科技厅重点研发计划项目(2022SF-217); 空军军医大学唐都医院创新基金资助项目(2017JCYJ007)
更新日期/Last Update: 2023-02-06