[1]朱东宁,安 庆.曲安奈德对特应性皮炎小鼠血清白细胞介素-33、白细胞介素-25、胸腺基质淋巴细胞生成素和Th1/Th2平衡影响的实验研究[J].陕西医学杂志,2023,52(1):7-11,27.[doi:DOI:10.3969/j.issn.1000-7377.2023.01.002]
　ZHU Dongning,AN Qing.Effects of triamcinolone acetonide on IL-33,IL-25,TSLP and Th1/Th2 balance in atopic dermatitis mice[J].,2023,52(1):7-11,27.[doi:DOI:10.3969/j.issn.1000-7377.2023.01.002]
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曲安奈德对特应性皮炎小鼠血清白细胞介素-33、白细胞介素-25、胸腺基质淋巴细胞生成素和Th1/Th2平衡影响的实验研究
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
52
期数:
2023年1期
页码:
7-11,27
栏目:
基础研究
出版日期:
2023-01-05

文章信息/Info

Title:
Effects of triamcinolone acetonide on IL-33,IL-25,TSLP and Th1/Th2 balance in atopic dermatitis mice
作者:
朱东宁1; 安 庆2
(1.西北妇女儿童医院皮肤科,陕西 西安 710061; 2.西北大学附属医院 西安市第三医院皮肤科,陕西 西安 710018)
Author(s):
ZHU Dongning; AN Qing
(Department of Dermatology,Northwest Women's and Children's Hospital,Xi'an 710061,China)
关键词:
曲安奈德;  特应性皮炎;  白细胞介素-33;  白细胞介素-25;  胸腺基质淋巴细胞生成素;  Th1/Th2平衡
Keywords:
Triamcinolone acetonide;  Atopic dermatitis;  IL-33;  IL-25;  Thymic stromal lymphopoietin;  Th1/Th2 balance
分类号:
R 758.2
DOI:
DOI:10.3969/j.issn.1000-7377.2023.01.002
文献标志码:
A
摘要:
目的:探讨曲安奈德对特应性皮炎小鼠白细胞介素-33(IL-33)、白细胞介素-25(IL-25)、胸腺基质淋巴细胞生成素(TSLP)和Th1/Th2平衡的影响。方法:选择ABLB/c雄性小鼠60只,分为对照组、模型组、阳性对照组、曲安奈德组高、中、低剂量,给予模型组、阳性对照组、高、中、低剂量组建立特应性皮炎小鼠模型。高剂量组腹腔注射3 μl 60 mg/ml曲安奈德,中剂量组腹腔注射3 μl 40 mg/ml曲安奈德,低剂量组腹腔注射3 μl 20 mg/ml曲安奈德,模型组、对照组腹腔注射3 μl的0.5%羧甲基纤维素钠蒸馏水,阳性组腹腔注射3 μl 10 mg/ml 泼尼松龙,每天1次,六组均给予15 d。对比六组小鼠的搔抓行为、特应性皮炎评分(SCORAD)指数评分、肥大细胞密度、皮肤组织厚度、小鼠血清中的IL-33、IL-25、TSLP水平及Th1/Th2细胞比例。结果:与对照组相比,阳性对照组、低、中、高剂量组、模型组的搔抓行为、SCORAD指数、肥大细胞密度、皮肤组织厚度、血清中的IL-33、IL-25、TSLP、Th2水平明显较高,Th1、Th1/Th2水平明显较低(均P<0.05); 与阳性对照组相比,低、中、高剂量组、模型组的搔抓行为、SCORAD指数、肥大细胞密度、皮肤组织厚度、血清中的IL-33、IL-25、TSLP、Th2水平明显较高,Th1、Th1/Th2水平明显较低(均P<0.05); 与高剂量组相比,中、低剂量组、模型组的搔抓行为、SCORAD指数、肥大细胞密度、皮肤组织厚度、血清中的IL-33、IL-25、TSLP、Th2水平明显较高,Th1、Th1/Th2水平明显较低(均P<0.05); 与低剂量组相比,模型组的搔抓行为、SCORAD指数、肥大细胞密度、皮肤组织厚度、血清中的IL-33、IL-25、TSLP、Th2水平明显较高,Th1、Th1/Th2水平明显较低(均P<0.05)。结论:曲安奈德可以通过抑制小鼠的IL-33、IL-25、TSLP水平,维持Th1/Th2平衡来改善特应性皮炎的皮肤损伤。
Abstract:
Objective:To investigate the effects of triamcinolone acetonide on the balance of IL-33,IL-25,TSLP and Th1/Th2 balance in mice with atopic dermatitis.Methods:A total of 60 male ABLB/c mice were divided into the control group,model group,positive control group,and high-,medium- and low-dose triamcinolone acetonide groups.The model group,positive control group,and high-,medium- and low-dose groups were given to establish the mouse model of atopic dermatitis.The high-dose group was intraperitoneally injected with 3 μl 60 mg/ml triamcinolone acetonide,the medium-dose group was intraperitoneally injected with 3 μl 40 mg/ml triamcinolone acetonide,the low-dose group was intraperitoneally injected with 3 μl 20 mg/ml triamcinolone acetonide,the model group and the control group were intraperitoneally injected with 0.5% sodium carboxymethyl cellulose distilled water,and the positive group received intraperitoneal injection of 3 μl 10 mg/ml prednisolone,once a day for 15 days.The scratching behavior,SCORAD index score,mast cell density,skin tissue thickness,serum levels of IL-33,IL-25,TSLP and Th1/Th2 cells were compared among the six groups.Results:Compared with the control group,the scratching behavior,SCORAD index,mast cell density,skin tissue thickness,serum levels of IL-33,IL-25,TSLP and Th2 were significantly higher in the positive control group,the low-,medium-- and high-dose groups,and the model group,while the levels of Th1 and Th1/Th2 were significantly lower(all P<0.05).Compared with the positive control group,the scratching behavior,SCORAD index,mast cell density,skin tissue thickness,serum levels of IL-33,IL-25,TSLP and Th2 were significantly higher in the low-,medium- and high-dose groups and the model group,while the levels of Th1 and Th1/Th2 were significantly lower(all P<0.05).Compared with the high-dose group,the scratching behavior,SCORAD index,mast cell density,skin tissue thickness,serum levels of IL-33,IL-25,TSLP and Th2 were significantly higher in the medium-dose group,low-dose group and model group,and the levels of Th1 and Th1/Th2 were significantly lower(all P<0.05).Compared with the low-dose group,the scratching behavior,SCORAD index,mast cell density,skin tissue thickness,serum levels of IL-33,IL-25,TSLP and Th2 in the model group were significantly higher,and the levels of Th1 and Th1/Th2 were significantly lower(all P<0.05).Conclusion:Triamcinolone acetonide can improve the skin injury of atopic dermatitis by inhibiting the levels of IL-33,IL-25 and TSLP in mice and maintaining Th1/Th2 balance.

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备注/Memo

备注/Memo:
基金项目:陕西省重点研发计划项目(2021SF-376)
更新日期/Last Update: 2022-12-28