[1]马瑞宁,侯惠莲,柳 雨,等.脑梗死半暗区星形胶质细胞形态学定量分析及临床意义[J].陕西医学杂志,2022,51(11):1445-1448.[doi:DOI:10.3969/j.issn.1000-7377.2022.11.030]
　MA Ruining,HOU Huilian,LIU Yu,et al.Quantitative analysis of astrocyte morphology in penumbra of cerebral infarction and its significance[J].,2022,51(11):1445-1448.[doi:DOI:10.3969/j.issn.1000-7377.2022.11.030]
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脑梗死半暗区星形胶质细胞形态学定量分析及临床意义
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《陕西医学杂志》[ISSN:1000-7377/CN:61-1281/TN]

卷:
51
期数:
2022年11期
页码:
1445-1448
栏目:
临床病理
出版日期:
2022-11-05

文章信息/Info

Title:
Quantitative analysis of astrocyte morphology in penumbra of cerebral infarction and its significance
作者:
马瑞宁; 侯惠莲; 柳 雨; 张冠军; 王鸿雁; 宫惠琳; 杨 喆
(西安交通大学第一附属医院病理科,陕西 西安 710061)
Author(s):
MA Ruining; HOU Huilian; LIU Yu; ZHANG Guanjun; WANG Hongyan; GONG Huilin; YANG Zhe
(Department of Pathology,the First Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710061,China)
关键词:
脑梗死;  半暗区;  星形胶质细胞;  胶质纤维酸性蛋白;  细胞体面积;  突起长度
Keywords:
Cerebral infarct;  Penumbra;  Astrocytes;  Glial fibrillary acidic protein;  Cell body area;  Process length
分类号:
R 743.3
DOI:
DOI:10.3969/j.issn.1000-7377.2022.11.030
文献标志码:
A
摘要:
目的:探讨脑梗死组织周围半暗区星形胶质细胞增生及形态学定量分析及意义。方法:回顾性分析临床病理资料完整的高血压引起脑梗死伴继发出血的患者行小骨窗微创开颅手术、石蜡包埋及切片标本63例,以每例梗死伴出血旁正常脑组织为对照组。采用免疫组织化学法检测人体脑梗死周围半暗区胶质纤维酸性蛋白(GFAP)的表达,用图像分析测定GFAP表达的星形胶质细胞数目、细胞体面积、突起长度的变化。结果:在周围正常脑组织中,GFAP标记的阳性星形细胞均匀分布,胞体较小,突起呈分支状较纤细,呈放射状分布; 在梗死灶周围半暗区,GFAP标记的阳性星形胶质细胞数目增多,细胞体面积增大,突起增长、互相交织成网,聚集在病灶边缘,并可见少量深入梗死灶。与周围正常脑组织比较,在脑梗死周围半暗区GFAP表达的星形胶质细胞阳性细胞数显著升高、星形胶质细胞体面积明显增大、突起明显增长,差异均有统计学意义(均P<0.01)。直线相关分析显示,在脑出血梗死周围半暗区,GFAP表达的星形胶质细胞阳性细胞数与星形胶质细胞体面积及细胞突起长度无相关性(均P>0.05)。结论:脑梗死组织半暗区星形胶质细胞反应性增生,这些反应性增生的星形胶质细胞形态学特征多样,可表现为细胞体积增大,或仅表现为细胞突起的伸长。间接说明星形胶质细胞参与了梗死灶神经修复和再生的过程。
Abstract:
Objective:To investigate the quantitative analysis and significance of astrocyte proliferation and morphology in penumbra around cerebral infarction.Methods:A total of 63 patients with cerebral infarction and secondary hemorrhage caused by hypertension with complete clinicopathological data were retrospectively analyzed.The small bone window minimally invasive craniotomy,paraffin embedding and sectioning specimens were performed in 63 patients.The normal brain tissue adjacent to the infarction and hemorrhage was taken as the control group.Immunohistochemistry was used to detect the expression of glial fibrillary acidic protein(GFAP)in penumbra around cerebral infarction.Image analysis was used to determine the changes of the number of GFAP-expressing astrocytes,cell body area and process length.Image analysis were used to measure the number,the cell body area and the process length of GFAP-expressing astrocytes.Results:In the surrounding normal brain tissue,the positive astrocytes labeled with GFAP were evenly distributed,the cell bodies were small,and the processes were branched and slender,with radial distribution.In the penumbra around the infarct,the number of GFAP labeled positive astrocytes increased,the cell body area increased,the processes increased,interwoven into a network,gathered at the edge of the lesion,and a small amount of deep infarct could be seen.Compared with the normal brain tissue,the number of astrocytes for the positive expression of GFAP,and the cell body area and the process length were increased significantly,respectively,in the cerebral infarcts with boundary zones of human tissues,the difference was statistically significant(all P<0.01).Linear correlation analysis showed that there was no correlation between the number of GFAP-positive astrocytes and the area of astrocytes and the length of astrocytes in the cerebral infarcts of human tissues(all P>0.05).Conclusion:Reactive proliferation of astrocytes in penumbra of cerebral infarction in human tissues.These reactive hyperplastic astrocytes are characterized by a variety of morphological features,which may be manifested by an increase in cell volume or only by the elongation of cell processes.Those indicate indirectly that astrocytes are involved in the process of nerve renovation and regeneration in the infarct.

参考文献/References:

[1] Qiao M,Bi Q,Fu P,et al.Previous hypertensive hemorrhage increases the risk for bleeding and ischemia for PCI patients on dual antiplatelet therapy[J].Neurol Res,2017,39(6):516-520.
[2] Zhang LM,Zhao XC,Zhan SQ,et al.Increased peri-operative crystalloid transfusion is associated witll better outcomes after spontaneous hypertensive putamen hemorrhage:A retrospective study[J].World Neurosurg,2017,21(98):140-145.
[3] Spellicy SE,Scheulin KM,Baker EW,et al.Semi-automated cell and tissue analyses reveal regionally specifific morphological alterations of immune and neural cells in a porcine middle cerebral artery occlusion model of stroke[J].Frontiers in Cellular Neuroscience,2021,14(1):1-21.
[4] Pekny M,Wilhelmsson U,Tatlisumak T,et al.Astrocyte activation and reactive gliosis:A new target in stroke?[J].Neuroscience Letters,2019,689(1):45-55.
[5] Ding R,Lin C,Wei S,et al.Therapeutic benefits of mesenchymal stromal cells in a rat model of hemoglobin-induced hypertensive intraeerebral hemorrhage[J].Mol Cells,2017,40(2):133-142.
[6] 叶建宇,孙自玉,胡薇薇.星形胶质细胞在脑梗死中的作用及相关治疗策略[J].浙江大学学报:医学版,2018,47(5):493-498.
[7] 刁东卫,方伯言,戚晓昆.一种新的脑膜脑脊髓炎:自身免疫性胶质纤维酸性蛋白星形胶质细胞病[J].中华神经科杂志,2017,50(5):399-400.
[8] 许子宁,李晓红,涂 悦.反应性星形胶质细胞转分化为神经元修复创伤性脑损伤的研究进展[J].中华创伤杂志,2019,35(5):435-440.
[9] 杨 薇,蔡新妤,高冉冉,等.丹参多酚酸注射液联合阿托伐他汀钙片治疗脑梗死疗效及对患者血液流变学影响[J].陕西中医,2022,43(3):334-337.
[10] 王安帮,杨少伟,高长庆,等.高血压脑出血血肿清除术后并发脑梗死相关因素分析[J].陕西医学杂志,2016,45(5):536-538.
[11] 贺东红,黄亮星.大动脉粥样硬化性前循环脑梗死患者临床特征及预后影响因素分析[J].陕西医学杂志,2020,49(7):800-803.
[12] 郭金赫,夏 青,郭永明.星型胶质细胞在缺血性脑卒中双重作用探究[J].辽宁中医药大学学报,2018,20(1):164-166.
[13] 陶仅德,李秋雨,漆 强,等.扩散张量成像、事件相关电位及血清胶质细胞纤维酸性蛋白在轻度创伤性脑损伤中的应用[J].中国临床研究,2017,30(3):294-297.
[14] 石代乐,高继英,杨李鹏,等.NT-proBNP、GFAP及HIF-1 α在脑出血患者炎性损伤中的表达及意义[J].中风与神经疾病杂志,2021,38(12):1099-1102.
[15] Rossi D.Astrocyte physiopathology:At the crossroads of intercellular networking,inflammation and cell death[J].Progress in Neurobiology,2015,130:86-120.
[16] Kamphuis W,Kooijman L,Orre M,et al.GFAP and vimentin deficiency alters gene expression in astrocytes and microglia in wild-type mice and changes the transcriptional response of reactive glia in mouse model for Alzheimers disease[J].Glia,2015,63(6):1036-1056.
[17] 何欣然,孙 浩,秦晓颖,等.星形胶质细胞在突触可塑性中的研究进展[J].神经损伤与功能重建,2022,17(4):215-217.
[18] 李利生,陆 阳,杨斯雷,等.一种神经元轴突变性的量化分析方法[J].遵义医学院学报,2017,40(4):440-445.
[19] 韩小伟,李 茗,张 冰.人工智能在脑卒中神经影像中的应用[J].协和医学杂志,2021,12(5):749- 754.
[20] 杜 瑶,李 欣,冉 利,等.星形胶质细胞在缺血性脑血管疾病中的双重作用[J].中风与神经疾病杂志,2018,35(9):841-844.

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更新日期/Last Update: 2022-11-09